Production of biosurfactant from locally isolated bacteria

In this study, a total of 176 isolates was obtained from two coastal sampling locations. Out of this total, 124 (70.4%) isolates were obtained from the seawaters of the coast of Kertih, Terengganu while the remaining 52 isolates (29.5 %) were from Kuantan, Pahang. Five bacterial strains previously i...

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Main Author:	Rihab Hussein, Jawad
Format:	Thesis
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/1968/1/Production%20of%20biosurfactant%20from%20locally%20isolated%20bacteria.pdf
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spelling	my-ump-ir.19682023-07-12T03:47:28Z Production of biosurfactant from locally isolated bacteria 2010-08 Rihab Hussein, Jawad TP Chemical technology In this study, a total of 176 isolates was obtained from two coastal sampling locations. Out of this total, 124 (70.4%) isolates were obtained from the seawaters of the coast of Kertih, Terengganu while the remaining 52 isolates (29.5 %) were from Kuantan, Pahang. Five bacterial strains previously isolated were selected for the screening of biosurfactant producer(s) via three different characterization tests for biosurfactant; (i) surface tension measurements, (ii) emulsification activity, and (iii) cetyltrimethylammonium bromide assay (CTAB) test. One isolate coded KRT-142 identified as Pseudomonas aeruginosa was chosen to be the best candidate for biosurfactant production. Biosurfactant productions by isolated bacteria were found to be growth-associated in all the conditions tested. Microbiological properties of strain KRT-142 were investigated. It was found that strain KRT-142 produces water soluble, greenish yellow fluorescent pigments on a nutrient agar plate. It is an aerobic, gram negative, straight rods, motile bacteria, and not surrounded by sheaths. Ethanol as a carbon source was found to support the highest growth (as measured by whole cell protein) followed by glycerol and glucose. Slight growth was also observed with crude oil. Decreasing growth was observed with tetradecane, 1-propanol, 1-butanol, sucrose and maltose. Ethanol yielded maximum biosurfactant production, reducing the surface tension to 43.3 mN/m. It was followed by glycerol, hexadecane and crude oil with surface tension reduction to 44.5, 49 and 53.5 mN/m, respectively. The highest emulsifying activity was 56% at 7h and 52.7% at 14h for ethanol. In the study of organic nitrogen sources, soytone supported the highest growth followed by peptone, meat extract, yeast extract, tryptone and casamino acid, Soytone yielded the highest biosurfactant production, followed by meat extract and tryptone. At the optimum conditions (35oC, 4% inoculum size, 100 rpm and pH 7.2), the surface tension reached a minimum of 30.76 mN/m, after 6h in the stationary growth phase. Stable and compact emulsification index (E24) was observed after 2h of cultivation, reaching a maximal value of 86% at 6h of incubation. 2010-08 Thesis http://umpir.ump.edu.my/id/eprint/1968/ http://umpir.ump.edu.my/id/eprint/1968/1/Production%20of%20biosurfactant%20from%20locally%20isolated%20bacteria.pdf pdf en public masters UNIVERSITI MALAYSIA PAHANG Faculty of Chemical & Natural Resources Engineering Hayder A., Abdul Bari
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Hayder A., Abdul Bari
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rihab Hussein, Jawad Production of biosurfactant from locally isolated bacteria
description	In this study, a total of 176 isolates was obtained from two coastal sampling locations. Out of this total, 124 (70.4%) isolates were obtained from the seawaters of the coast of Kertih, Terengganu while the remaining 52 isolates (29.5 %) were from Kuantan, Pahang. Five bacterial strains previously isolated were selected for the screening of biosurfactant producer(s) via three different characterization tests for biosurfactant; (i) surface tension measurements, (ii) emulsification activity, and (iii) cetyltrimethylammonium bromide assay (CTAB) test. One isolate coded KRT-142 identified as Pseudomonas aeruginosa was chosen to be the best candidate for biosurfactant production. Biosurfactant productions by isolated bacteria were found to be growth-associated in all the conditions tested. Microbiological properties of strain KRT-142 were investigated. It was found that strain KRT-142 produces water soluble, greenish yellow fluorescent pigments on a nutrient agar plate. It is an aerobic, gram negative, straight rods, motile bacteria, and not surrounded by sheaths. Ethanol as a carbon source was found to support the highest growth (as measured by whole cell protein) followed by glycerol and glucose. Slight growth was also observed with crude oil. Decreasing growth was observed with tetradecane, 1-propanol, 1-butanol, sucrose and maltose. Ethanol yielded maximum biosurfactant production, reducing the surface tension to 43.3 mN/m. It was followed by glycerol, hexadecane and crude oil with surface tension reduction to 44.5, 49 and 53.5 mN/m, respectively. The highest emulsifying activity was 56% at 7h and 52.7% at 14h for ethanol. In the study of organic nitrogen sources, soytone supported the highest growth followed by peptone, meat extract, yeast extract, tryptone and casamino acid, Soytone yielded the highest biosurfactant production, followed by meat extract and tryptone. At the optimum conditions (35oC, 4% inoculum size, 100 rpm and pH 7.2), the surface tension reached a minimum of 30.76 mN/m, after 6h in the stationary growth phase. Stable and compact emulsification index (E24) was observed after 2h of cultivation, reaching a maximal value of 86% at 6h of incubation.
format	Thesis
qualification_level	Master's degree
author	Rihab Hussein, Jawad
author_facet	Rihab Hussein, Jawad
author_sort	Rihab Hussein, Jawad
title	Production of biosurfactant from locally isolated bacteria
title_short	Production of biosurfactant from locally isolated bacteria
title_full	Production of biosurfactant from locally isolated bacteria
title_fullStr	Production of biosurfactant from locally isolated bacteria
title_full_unstemmed	Production of biosurfactant from locally isolated bacteria
title_sort	production of biosurfactant from locally isolated bacteria
granting_institution	UNIVERSITI MALAYSIA PAHANG
granting_department	Faculty of Chemical & Natural Resources Engineering
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/1968/1/Production%20of%20biosurfactant%20from%20locally%20isolated%20bacteria.pdf
_version_	1783731886845067264

Production of biosurfactant from locally isolated bacteria

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