Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles

The production of x-ray sheets can utilize up to 1000 tons of silver chemically produced worldwide. The pollution occurs when the used x-ray sheets are not recycled. Microbial management could be used to manage this problem in order to remove and neutralize the pollutants from contamination. This st...

Full description

Saved in:

Bibliographic Details
Main Author:	Siti Hajar, Mohd Rasdi
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/23261/1/Isolation%20and%20characterization%20of%20bacterial%20isolates%20for%20bioremediation%20of%20disposed%20x-ray%20sheets%20to%20produce%20silver%20nanoparticles.wm.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ump-ir.23261
record_format	uketd_dc
spelling	my-ump-ir.232612023-02-15T07:01:24Z Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles 2018-05 Siti Hajar, Mohd Rasdi QR Microbiology The production of x-ray sheets can utilize up to 1000 tons of silver chemically produced worldwide. The pollution occurs when the used x-ray sheets are not recycled. Microbial management could be used to manage this problem in order to remove and neutralize the pollutants from contamination. This study aims to examine the microbial management of disposed x-ray sheets in silver nanoparticles (SNPs) production and the role of the nitrate reductase (NR) enzyme involved in the processes. In this research, disposed x-ray sheets were used as the sole carbon source to synthesis SNPs and catalyse the reduction of silver nitrate to silver nitrite. Different bacterial isolates which had been chosen from the most potent isolates were used and being optimized by using several parameters including incubation condition, substrate concentration, incubation period, pH values and nitrogen source. Then, the protein quantification and enzyme activity were analyzed by using microplate reader. For characterization of the SNPs, the X-Ray Diffraction (XRD) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis were used. The results revealed that the most potent isolates CL4C and GL7 showed the average size of SNPs produced was up to 19.53 nm and 52.35 nm respectively. In addition, NR activity was found to be optimum at static incubation condition, 15 days incubation period in darkness and using sodium nitrate as the nitrogen source. Then, substrate concentration of CL4C and GL7 isolates found to be optimum when 0.5 and 0.8g of disposed x-ray sheets were added respectively. The optimum pH values of CL4C and GL7 were exhibited to be 7.0 and 8.0, respectively, with the enzyme activity and protein content up to 69.952±0.090 IU/ml and 1.139±0.029 mg/ml for CL4C. Meanwhile, the NR activity of the GL7 isolate was 108.882±0.354 IU/ml with the protein content up to 1.454±0.024 mg/ml. Consequently, the CL4C isolate was predicted to be Morganella morganii while GL7 was predicted to be Enterobacter aerogenes by using a Biolog analysis. It can be concluded that, by using the biological method, it requires less toxic chemicals and as a good alternative for bioremediation. In this study, the impact of having isolated CL4C and GL7 as the most potent microbe which able to use disposed x-ray sheets as substrate was a new finding in this research area because there was no previous study yet regarding the uses of this substrate. 2018-05 Thesis http://umpir.ump.edu.my/id/eprint/23261/ http://umpir.ump.edu.my/id/eprint/23261/1/Isolation%20and%20characterization%20of%20bacterial%20isolates%20for%20bioremediation%20of%20disposed%20x-ray%20sheets%20to%20produce%20silver%20nanoparticles.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology Essam, A. Makky
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Essam, A. Makky
topic	QR Microbiology
spellingShingle	QR Microbiology Siti Hajar, Mohd Rasdi Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
description	The production of x-ray sheets can utilize up to 1000 tons of silver chemically produced worldwide. The pollution occurs when the used x-ray sheets are not recycled. Microbial management could be used to manage this problem in order to remove and neutralize the pollutants from contamination. This study aims to examine the microbial management of disposed x-ray sheets in silver nanoparticles (SNPs) production and the role of the nitrate reductase (NR) enzyme involved in the processes. In this research, disposed x-ray sheets were used as the sole carbon source to synthesis SNPs and catalyse the reduction of silver nitrate to silver nitrite. Different bacterial isolates which had been chosen from the most potent isolates were used and being optimized by using several parameters including incubation condition, substrate concentration, incubation period, pH values and nitrogen source. Then, the protein quantification and enzyme activity were analyzed by using microplate reader. For characterization of the SNPs, the X-Ray Diffraction (XRD) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis were used. The results revealed that the most potent isolates CL4C and GL7 showed the average size of SNPs produced was up to 19.53 nm and 52.35 nm respectively. In addition, NR activity was found to be optimum at static incubation condition, 15 days incubation period in darkness and using sodium nitrate as the nitrogen source. Then, substrate concentration of CL4C and GL7 isolates found to be optimum when 0.5 and 0.8g of disposed x-ray sheets were added respectively. The optimum pH values of CL4C and GL7 were exhibited to be 7.0 and 8.0, respectively, with the enzyme activity and protein content up to 69.952±0.090 IU/ml and 1.139±0.029 mg/ml for CL4C. Meanwhile, the NR activity of the GL7 isolate was 108.882±0.354 IU/ml with the protein content up to 1.454±0.024 mg/ml. Consequently, the CL4C isolate was predicted to be Morganella morganii while GL7 was predicted to be Enterobacter aerogenes by using a Biolog analysis. It can be concluded that, by using the biological method, it requires less toxic chemicals and as a good alternative for bioremediation. In this study, the impact of having isolated CL4C and GL7 as the most potent microbe which able to use disposed x-ray sheets as substrate was a new finding in this research area because there was no previous study yet regarding the uses of this substrate.
format	Thesis
qualification_level	Master's degree
author	Siti Hajar, Mohd Rasdi
author_facet	Siti Hajar, Mohd Rasdi
author_sort	Siti Hajar, Mohd Rasdi
title	Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
title_short	Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
title_full	Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
title_fullStr	Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
title_full_unstemmed	Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
title_sort	isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23261/1/Isolation%20and%20characterization%20of%20bacterial%20isolates%20for%20bioremediation%20of%20disposed%20x-ray%20sheets%20to%20produce%20silver%20nanoparticles.wm.pdf
_version_	1783732057377079296

Isolation and characterization of bacterial isolates for bioremediation of disposed x-ray sheets to produce silver nanoparticles

Similar Items