Screening and optimisation study on antioxidant extracts from selected lamiaceae plants

This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis was to select the plant materials of the...

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Main Author:	Mohd Shaiful Azman, Abdul Rahim
Format:	Thesis
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF
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spelling	my-ump-ir.27332015-03-03T07:58:12Z Screening and optimisation study on antioxidant extracts from selected lamiaceae plants 2010-12 Mohd Shaiful Azman, Abdul Rahim TP Chemical technology This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis was to select the plant materials of the selected Lamiaceae species that gave the highest antioxidant activity, highest phenols content and best mineral profiles. This thesis presents the optimised levels of experimental variables that gave the highest antioxidant activity-yield. This thesis also presents the optimum levels of the operational variables that influence the spray drying of the liquid extract of the Lamiaceae plant. Fresh Plecranthus amboinicus Lour. Indonesia (PALl) samples were randomly collected from Jakarta, Indonesia and samples of Plecranthus amboinicus Lour. Malaysia (PALM), Pogostemon cablin Benth. (PCB), SOlenostemon scutellariodes Benth. Red (SSR) and Solenostemon scutellariodes Mix Colour (SSMC) were randomly collected from Kuantan, Malaysia. The extracted samples were assessed using 1,1-diphenyl-2- picry1hydrazyl (DPPH) free radical scavengiig assay for determination of antioxidant activity. Folin Ciocalteau method was chosen for total phenols determination, while the essential minerals were analysed by using atomic absorption spectrometer. The 22 factorial design of experiment was used in the optimisation of extraction of antioxidants by boiling. The method of Path of Steepest Ascent was used to maximise the antioxidant activity in the spray dried extract. The obtained results indicated that the - leaves tissues of PCB samples exhibited the significant 6<0.05) highest antioxidant activity (63.18%) and highest phenols content (7.64 mg/g fresh weight) while the highest total minerals content was observed in the PALl leaves (38.41 ppm). For optimisation of extraction of antioxidant by boiling, the theOretical maximum point has the boiling temperature (T) at 116.18°C and boiling time duration (t) at 107.81 minutes which gave an antioxidant activity-yield of 65.153 ' %. The combination of levels of operational variables where the spray dryer was operated at X1 (temperature) = 177.65°C and X2 (concentration of maltodextrin) = 48.65% gave the highest yield of antioxidant activity of spray dried extract. This research suggests a chemical basis for some of the health benefits claimed for in folk medicine and warrant further studies to develop it as a natural functional food. 2010-12 Thesis http://umpir.ump.edu.my/id/eprint/2733/ http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF application/pdf en public masters Universiti Malaysia Pahang Chemical Industry
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd Shaiful Azman, Abdul Rahim Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
description	This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis was to select the plant materials of the selected Lamiaceae species that gave the highest antioxidant activity, highest phenols content and best mineral profiles. This thesis presents the optimised levels of experimental variables that gave the highest antioxidant activity-yield. This thesis also presents the optimum levels of the operational variables that influence the spray drying of the liquid extract of the Lamiaceae plant. Fresh Plecranthus amboinicus Lour. Indonesia (PALl) samples were randomly collected from Jakarta, Indonesia and samples of Plecranthus amboinicus Lour. Malaysia (PALM), Pogostemon cablin Benth. (PCB), SOlenostemon scutellariodes Benth. Red (SSR) and Solenostemon scutellariodes Mix Colour (SSMC) were randomly collected from Kuantan, Malaysia. The extracted samples were assessed using 1,1-diphenyl-2- picry1hydrazyl (DPPH) free radical scavengiig assay for determination of antioxidant activity. Folin Ciocalteau method was chosen for total phenols determination, while the essential minerals were analysed by using atomic absorption spectrometer. The 22 factorial design of experiment was used in the optimisation of extraction of antioxidants by boiling. The method of Path of Steepest Ascent was used to maximise the antioxidant activity in the spray dried extract. The obtained results indicated that the - leaves tissues of PCB samples exhibited the significant 6<0.05) highest antioxidant activity (63.18%) and highest phenols content (7.64 mg/g fresh weight) while the highest total minerals content was observed in the PALl leaves (38.41 ppm). For optimisation of extraction of antioxidant by boiling, the theOretical maximum point has the boiling temperature (T) at 116.18°C and boiling time duration (t) at 107.81 minutes which gave an antioxidant activity-yield of 65.153 ' %. The combination of levels of operational variables where the spray dryer was operated at X1 (temperature) = 177.65°C and X2 (concentration of maltodextrin) = 48.65% gave the highest yield of antioxidant activity of spray dried extract. This research suggests a chemical basis for some of the health benefits claimed for in folk medicine and warrant further studies to develop it as a natural functional food.
format	Thesis
qualification_level	Master's degree
author	Mohd Shaiful Azman, Abdul Rahim
author_facet	Mohd Shaiful Azman, Abdul Rahim
author_sort	Mohd Shaiful Azman, Abdul Rahim
title	Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
title_short	Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
title_full	Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
title_fullStr	Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
title_full_unstemmed	Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
title_sort	screening and optimisation study on antioxidant extracts from selected lamiaceae plants
granting_institution	Universiti Malaysia Pahang
granting_department	Chemical Industry
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF
_version_	1783731891536396288

Screening and optimisation study on antioxidant extracts from selected lamiaceae plants

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