Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves

Aquilaria species contains variety of secondary metabolites including various phenolic compounds which have been reported as excellent antioxidants. A study on phytochemical and antioxidant properties of Aquilaria malaccensis from Thymelaeaceae family was performed. The extraction of phytochemicals...

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Main Author:	Nik Noor Asma, Nik Wil
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/31272/1/Chemical%20constituents%20and%20antioxidant%20activities%20of%20aquilaria%20malaccensis%20lamk.%20%28agarwood%29%20leaves.wm.pdf
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spelling	my-ump-ir.312722023-05-17T07:32:30Z Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves 2019-08 Nik Noor Asma, Nik Wil Q Science (General) T Technology (General) Aquilaria species contains variety of secondary metabolites including various phenolic compounds which have been reported as excellent antioxidants. A study on phytochemical and antioxidant properties of Aquilaria malaccensis from Thymelaeaceae family was performed. The extraction of phytochemicals from the leaves were performed using conventional extraction methods: reflux extraction using water and maceration using methanol to obtain water extract (WE) and methanol extract (ME), respectively. Solvent–solvent extraction, fractionation and separation using different chromatographic techniques (column chromatography and thin layer chromatography) were used for isolation of pure compounds. In order to identify the volatile aroma compounds in the leaves of A. malaccensis, two methods were used namely solid-phase microextraction (SPME) and direct thermal desorption (DTD), in combination with gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. The ultra-highperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF/MS) was used for determination of non-volatile chemical compounds present in the leaves. The potential antioxidative activity of the leaves extracts of A. malaccensis was evaluated via radical scavenging assay (DPPH) and copper reducing antioxidant capacity (CUPRAC) assays. Significant differences were based on p values where p<0.05 were considered significantly different and vice-versa. Some of the commonly identified chemical components were n-hexadecanoic acid, eudesmol and oxo-agarospirol. WE and ME extracts with the highest total phenolic contents (191.005 ± 0.002 and 177.927 ± 0.001 mg of GAEs/g extract) showed strong reducing power and scavenging radical activity. Friedelanol and friedelin were isolated from the fractionation of hexane and dichloromethane extracts, respectively. The structure of the isolated compounds was elucidated by using spectroscopic methods namely 1D (1H, 13C, DEPTQ), 2D (COSY, HSQC, HMBC) NMR, MS, UV, FTIR and by comparison with literature values of published data spectra. 2019-08 Thesis http://umpir.ump.edu.my/id/eprint/31272/ http://umpir.ump.edu.my/id/eprint/31272/1/Chemical%20constituents%20and%20antioxidant%20activities%20of%20aquilaria%20malaccensis%20lamk.%20%28agarwood%29%20leaves.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology Tajuddin, Saiful Nizam
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Tajuddin, Saiful Nizam
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Nik Noor Asma, Nik Wil Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
description	Aquilaria species contains variety of secondary metabolites including various phenolic compounds which have been reported as excellent antioxidants. A study on phytochemical and antioxidant properties of Aquilaria malaccensis from Thymelaeaceae family was performed. The extraction of phytochemicals from the leaves were performed using conventional extraction methods: reflux extraction using water and maceration using methanol to obtain water extract (WE) and methanol extract (ME), respectively. Solvent–solvent extraction, fractionation and separation using different chromatographic techniques (column chromatography and thin layer chromatography) were used for isolation of pure compounds. In order to identify the volatile aroma compounds in the leaves of A. malaccensis, two methods were used namely solid-phase microextraction (SPME) and direct thermal desorption (DTD), in combination with gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. The ultra-highperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF/MS) was used for determination of non-volatile chemical compounds present in the leaves. The potential antioxidative activity of the leaves extracts of A. malaccensis was evaluated via radical scavenging assay (DPPH) and copper reducing antioxidant capacity (CUPRAC) assays. Significant differences were based on p values where p<0.05 were considered significantly different and vice-versa. Some of the commonly identified chemical components were n-hexadecanoic acid, eudesmol and oxo-agarospirol. WE and ME extracts with the highest total phenolic contents (191.005 ± 0.002 and 177.927 ± 0.001 mg of GAEs/g extract) showed strong reducing power and scavenging radical activity. Friedelanol and friedelin were isolated from the fractionation of hexane and dichloromethane extracts, respectively. The structure of the isolated compounds was elucidated by using spectroscopic methods namely 1D (1H, 13C, DEPTQ), 2D (COSY, HSQC, HMBC) NMR, MS, UV, FTIR and by comparison with literature values of published data spectra.
format	Thesis
qualification_level	Master's degree
author	Nik Noor Asma, Nik Wil
author_facet	Nik Noor Asma, Nik Wil
author_sort	Nik Noor Asma, Nik Wil
title	Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
title_short	Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
title_full	Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
title_fullStr	Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
title_full_unstemmed	Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves
title_sort	chemical constituents and antioxidant activities of aquilaria malaccensis lamk. (agarwood) leaves
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31272/1/Chemical%20constituents%20and%20antioxidant%20activities%20of%20aquilaria%20malaccensis%20lamk.%20%28agarwood%29%20leaves.wm.pdf
_version_	1783732162841804800

Chemical constituents and antioxidant activities of Aquilaria malaccensis Lamk. (agarwood) leaves

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