Optimization process of binahong extraction and characterization study

Binahong is Anredera cordifolia (Ten) Steenis is including ones a medicinal plant and being used as herbal medicine. These compounds include saponin and polyphenols. They involve a lot of activities, one of these is the antimicrobial activity that will be used to treat infectious diseases by microor...

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Main Author:	Sri Murni, Astuti
Format:	Thesis
Language:	English
Published:	2011
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9105/1/CD8508%20%40%20178.pdf
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spelling	my-ump-ir.91052021-08-24T01:43:01Z Optimization process of binahong extraction and characterization study 2011-09 Sri Murni, Astuti TP Chemical technology Binahong is Anredera cordifolia (Ten) Steenis is including ones a medicinal plant and being used as herbal medicine. These compounds include saponin and polyphenols. They involve a lot of activities, one of these is the antimicrobial activity that will be used to treat infectious diseases by microorganism agents, resistance by microorganisms produce. However, the compound of Binahong plant is not well documented and scientific evidence is limited to establish as medicinal plant. The research proposed to know optimization process of Binahong extraction and characterization study. The samples are leaves, tuber, stems, flower and local capsule of Binahong carried out optimization processing of Total Phenol Content (TPC) by using UV Vis-Spectrophotometer and tetracycline activity (TCA) by bioassay. The optimization extraction of processes was evaluated with the aid of Design Expert version 6.0.8 using response surface methodology (RSM). The characterization of TPC and TFC by using UV Vis-Spectrophotometer, the antibiotic activity analysed by bioassay, the bioactive compounds from a volatile oil compound analysed by using GCMS, and accumulation of heavy metals on Binahong plant analysed by Inductively Coupled Plasma Mass Spectrometer (ICPMS). The results of TPC in leaves were optimum temperature at 65OC and concentration between 45 mg/l to 62.5 mg/l, in actual factor time 17.50 minutes. While the point optimum of tetracycline activity (TCA) from tuber, at temperature 72.50 OC for time 32.50 minute. The phenolic and flavonoid compound were higher in flower extract, then leaves and tuber. For the local capsule of Binahong was higher than leaves and tuber. The result of compounds analysed by using GC-MS were 68 kind of compounds. The heavy metals result were found Mg (38.80 ± 0.10) ppm; Cu (1.542 ± 0.01) ppm; Se (0.023 ± 0.002) ppm and Zn (7.310 ± 0.02) ppm, but in tuber was Mg (10.10 ± 0.02) ppm, Cu (0.652 ± 0.01) ppm, Se (<1.636 ± 0.003) ppm and Zn: 2.729 ± 0.01) ppm. However, from contamination of heavy metal such as in the leaves, As (0.008 ± 0.01) ppm, Cd (0.003 ± 0.02) ppm, Pb (0.0629 ± 0.03) ppm and Cr: (0.098 ± 0.01) ppm. While from a tuber was: As (< 0.017 ± 0.01) ppm, Cd (< 0.015 ± 0.03) ppm, Pb (0.0091 ± 0.02) ppm and Cr 0.036 ± 0.03 ppm. The Binahong plant as medicinal herbal has the high antioxidant activity such as total phenolic, flavonoid and saponin compound. The plant is also contained group of fatty acid, phytosterol, tocopherol, polyphenols, phytol and antibiotic activity. The Binahong plant was safe for humans consumption and has a lot of benefit for human health 2011-09 Thesis http://umpir.ump.edu.my/id/eprint/9105/ http://umpir.ump.edu.my/id/eprint/9105/1/CD8508%20%40%20178.pdf application/pdf en public masters Universiti Malaysia Pahang Faculty Of Chemical & Natural Resources Engineering
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Sri Murni, Astuti Optimization process of binahong extraction and characterization study
description	Binahong is Anredera cordifolia (Ten) Steenis is including ones a medicinal plant and being used as herbal medicine. These compounds include saponin and polyphenols. They involve a lot of activities, one of these is the antimicrobial activity that will be used to treat infectious diseases by microorganism agents, resistance by microorganisms produce. However, the compound of Binahong plant is not well documented and scientific evidence is limited to establish as medicinal plant. The research proposed to know optimization process of Binahong extraction and characterization study. The samples are leaves, tuber, stems, flower and local capsule of Binahong carried out optimization processing of Total Phenol Content (TPC) by using UV Vis-Spectrophotometer and tetracycline activity (TCA) by bioassay. The optimization extraction of processes was evaluated with the aid of Design Expert version 6.0.8 using response surface methodology (RSM). The characterization of TPC and TFC by using UV Vis-Spectrophotometer, the antibiotic activity analysed by bioassay, the bioactive compounds from a volatile oil compound analysed by using GCMS, and accumulation of heavy metals on Binahong plant analysed by Inductively Coupled Plasma Mass Spectrometer (ICPMS). The results of TPC in leaves were optimum temperature at 65OC and concentration between 45 mg/l to 62.5 mg/l, in actual factor time 17.50 minutes. While the point optimum of tetracycline activity (TCA) from tuber, at temperature 72.50 OC for time 32.50 minute. The phenolic and flavonoid compound were higher in flower extract, then leaves and tuber. For the local capsule of Binahong was higher than leaves and tuber. The result of compounds analysed by using GC-MS were 68 kind of compounds. The heavy metals result were found Mg (38.80 ± 0.10) ppm; Cu (1.542 ± 0.01) ppm; Se (0.023 ± 0.002) ppm and Zn (7.310 ± 0.02) ppm, but in tuber was Mg (10.10 ± 0.02) ppm, Cu (0.652 ± 0.01) ppm, Se (<1.636 ± 0.003) ppm and Zn: 2.729 ± 0.01) ppm. However, from contamination of heavy metal such as in the leaves, As (0.008 ± 0.01) ppm, Cd (0.003 ± 0.02) ppm, Pb (0.0629 ± 0.03) ppm and Cr: (0.098 ± 0.01) ppm. While from a tuber was: As (< 0.017 ± 0.01) ppm, Cd (< 0.015 ± 0.03) ppm, Pb (0.0091 ± 0.02) ppm and Cr 0.036 ± 0.03 ppm. The Binahong plant as medicinal herbal has the high antioxidant activity such as total phenolic, flavonoid and saponin compound. The plant is also contained group of fatty acid, phytosterol, tocopherol, polyphenols, phytol and antibiotic activity. The Binahong plant was safe for humans consumption and has a lot of benefit for human health
format	Thesis
qualification_level	Master's degree
author	Sri Murni, Astuti
author_facet	Sri Murni, Astuti
author_sort	Sri Murni, Astuti
title	Optimization process of binahong extraction and characterization study
title_short	Optimization process of binahong extraction and characterization study
title_full	Optimization process of binahong extraction and characterization study
title_fullStr	Optimization process of binahong extraction and characterization study
title_full_unstemmed	Optimization process of binahong extraction and characterization study
title_sort	optimization process of binahong extraction and characterization study
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty Of Chemical & Natural Resources Engineering
publishDate	2011
url	http://umpir.ump.edu.my/id/eprint/9105/1/CD8508%20%40%20178.pdf
_version_	1783731951458320384

Optimization process of binahong extraction and characterization study

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