Production of succinic acid from glycerol and glycerol waste

Succinic acid is extensively used globally for manufacture of synthetic resin, biodegradable polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced through petrochemical route. The process is non-renewable and will affected pollution in the environment. Therefore,...

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Main Author:	Nor Hidawati, Elias
Format:	Thesis
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9110/1/CD8503%20%40%20190.pdf
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id	my-ump-ir.9110
record_format	uketd_dc
spelling	my-ump-ir.91102021-08-24T01:46:25Z Production of succinic acid from glycerol and glycerol waste 2012-01 Nor Hidawati, Elias TP Chemical technology Succinic acid is extensively used globally for manufacture of synthetic resin, biodegradable polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced through petrochemical route. The process is non-renewable and will affected pollution in the environment. Therefore, more attention has been focused towards a friendly environment processes for succinic acid production by way of fermentation process; which is highly recommended nowadays. In this research, studies been done to produce succinic acid through anaerobic fermentation of glycerol waste using Escherichia coli and to investigate the effect of each factors that influence succinic acid production. The most influential parameter for succinic acid production obtained via preliminary study of parameter range selection were glycerol concentration, tryptone concentration, sodium carbonate (Na2CO3) concentration , inoculums density, pH and incubation period. This experiment has produced 0.973g/l succinic acid using One-factor-at-a-time (OFAT) approach from commercial glycerol sample. These six variables screened to select the most significant factor affected succinic acid production using fractional factorial design (FFD) based on first order model term. Four factors selected while, two factors eliminated for the next stage of experiment. Subsequently, full factor central composite designs (CCD) falling under RSM employed to develop a mathematical correlation model between the significant factors for physio-chemical variables of succinic acid production. The experiment has produced 7.55g/l succinic acid from commercial glycerol sample. Besides, a second order polynomial regression fitted and the model was adequate by R-squared 0.9864. Meanwhile, under the optimum level, it is about 7.34g/l succinic acid being produced from commercial glycerol and 7.32g/l of succinic acid produced from recovered glycerol. Overall, this research shows glycerine pitch can be used safely and effective as a carbon source for Escherichia coli to produce succinic acid. In addition, this research provides an exciting opportunity to transform low-grade glycerine pitch into highly demanded chemical such succinic acid 2012-01 Thesis http://umpir.ump.edu.my/id/eprint/9110/ http://umpir.ump.edu.my/id/eprint/9110/1/CD8503%20%40%20190.pdf application/pdf en public masters Universiti Malaysia Pahang Faculty Of Chemical & Natural Resources Engineering
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nor Hidawati, Elias Production of succinic acid from glycerol and glycerol waste
description	Succinic acid is extensively used globally for manufacture of synthetic resin, biodegradable polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced through petrochemical route. The process is non-renewable and will affected pollution in the environment. Therefore, more attention has been focused towards a friendly environment processes for succinic acid production by way of fermentation process; which is highly recommended nowadays. In this research, studies been done to produce succinic acid through anaerobic fermentation of glycerol waste using Escherichia coli and to investigate the effect of each factors that influence succinic acid production. The most influential parameter for succinic acid production obtained via preliminary study of parameter range selection were glycerol concentration, tryptone concentration, sodium carbonate (Na2CO3) concentration , inoculums density, pH and incubation period. This experiment has produced 0.973g/l succinic acid using One-factor-at-a-time (OFAT) approach from commercial glycerol sample. These six variables screened to select the most significant factor affected succinic acid production using fractional factorial design (FFD) based on first order model term. Four factors selected while, two factors eliminated for the next stage of experiment. Subsequently, full factor central composite designs (CCD) falling under RSM employed to develop a mathematical correlation model between the significant factors for physio-chemical variables of succinic acid production. The experiment has produced 7.55g/l succinic acid from commercial glycerol sample. Besides, a second order polynomial regression fitted and the model was adequate by R-squared 0.9864. Meanwhile, under the optimum level, it is about 7.34g/l succinic acid being produced from commercial glycerol and 7.32g/l of succinic acid produced from recovered glycerol. Overall, this research shows glycerine pitch can be used safely and effective as a carbon source for Escherichia coli to produce succinic acid. In addition, this research provides an exciting opportunity to transform low-grade glycerine pitch into highly demanded chemical such succinic acid
format	Thesis
qualification_level	Master's degree
author	Nor Hidawati, Elias
author_facet	Nor Hidawati, Elias
author_sort	Nor Hidawati, Elias
title	Production of succinic acid from glycerol and glycerol waste
title_short	Production of succinic acid from glycerol and glycerol waste
title_full	Production of succinic acid from glycerol and glycerol waste
title_fullStr	Production of succinic acid from glycerol and glycerol waste
title_full_unstemmed	Production of succinic acid from glycerol and glycerol waste
title_sort	production of succinic acid from glycerol and glycerol waste
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty Of Chemical & Natural Resources Engineering
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/9110/1/CD8503%20%40%20190.pdf
_version_	1783731951699492864

Production of succinic acid from glycerol and glycerol waste

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