Bacteria-sponge interaction : Existence of possible chemical correlation

The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon s...

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Main Author:	Koh, Johleen Tse Boon
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	QL360-599.82 Invertebrates
Online Access:	https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf
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id	my-ums-ep.38031
record_format	uketd_dc
spelling	my-ums-ep.380312024-01-26T07:20:19Z Bacteria-sponge interaction : Existence of possible chemical correlation 2010 Koh, Johleen Tse Boon QL360-599.82 Invertebrates The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based _on extensive H 1-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AAl were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AAl and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AAl. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp. 2010 Thesis https://eprints.ums.edu.my/id/eprint/38031/ https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Saba Institut Penyelidikan Marin Borneo
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QL360-599.82 Invertebrates
spellingShingle	QL360-599.82 Invertebrates Koh, Johleen Tse Boon Bacteria-sponge interaction : Existence of possible chemical correlation
description	The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based _on extensive H 1-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AAl were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AAl and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AAl. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp.
format	Thesis
qualification_level	Master's degree
author	Koh, Johleen Tse Boon
author_facet	Koh, Johleen Tse Boon
author_sort	Koh, Johleen Tse Boon
title	Bacteria-sponge interaction : Existence of possible chemical correlation
title_short	Bacteria-sponge interaction : Existence of possible chemical correlation
title_full	Bacteria-sponge interaction : Existence of possible chemical correlation
title_fullStr	Bacteria-sponge interaction : Existence of possible chemical correlation
title_full_unstemmed	Bacteria-sponge interaction : Existence of possible chemical correlation
title_sort	bacteria-sponge interaction : existence of possible chemical correlation
granting_institution	Universiti Malaysia Saba
granting_department	Institut Penyelidikan Marin Borneo
publishDate	2010
url	https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf
_version_	1794022701900234752

Bacteria-sponge interaction : Existence of possible chemical correlation

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