Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah

A radical rise posting in the incidence of wilting and dieback diseases induced by plant pathogenic fungi Ceratocystis sp. in commercially planted Acacia mangium are major problem faced by industrial plantations such as Acacia Forest Industry (AFI) Plantation. Two types of Ceratocystis isolates;Cera...

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Main Author:	Khesnii Shiv Ram
Format:	Thesis
Language:	English English
Published:	2019
Subjects:	SB317.5-319.864 Horticulture Horticultural crops
Online Access:	https://eprints.ums.edu.my/id/eprint/41351/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41351/2/FULLTEXT.pdf
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id	my-ums-ep.41351
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spelling	my-ums-ep.413512024-10-25T01:21:02Z Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah 2019 Khesnii Shiv Ram SB317.5-319.864 Horticulture. Horticultural crops A radical rise posting in the incidence of wilting and dieback diseases induced by plant pathogenic fungi Ceratocystis sp. in commercially planted Acacia mangium are major problem faced by industrial plantations such as Acacia Forest Industry (AFI) Plantation. Two types of Ceratocystis isolates;Ceratocystis manginecans (AFI/CM/001) and Ceratocystis acaciivora (AFI/CA/001) are recognized and collected from AFI Plantation in Pitas, Sabah. The general aim is to scientifically determine the immensity of Ceratocystis isolates to induce disease on plantation grown A. mangium using pathogenicity and interaction study. On this objective, field inoculation is done in the plantation nursery and interaction behavior is to be observed in-vitro. In field inoculation, 100 A. mangium seedlings were inoculated with C. manginecans and C. acaciivora to observe the ability of both fungi to induce diseases for 6 weeks. The efficacy study shows both fungi have capability to enhance lesion on A. mangium seedlings. C. manginecans isolate produced longer lesions (mean=47.103) compared to C. acaciivora isolate (mean=42.940), the difference is not significant (p=0.148, F=2.099). Yet, C. acaciivora showed a more linear growth in lesion increment compared to C. manginecans by weekly observation. Dual culture method was used to assess the ability of 6 test isolates which were identified during the isolation of infected A. mangium sample to control C. manginecans and C. acaciivora, which causes the wilting and dieback diseases. The highest percentage inhibition of radical growth (PIRG) values was observed with isolate KHES/AFI-UMS/D001; Trichoderma sp (60.00%; C. manginecans and 53.33%; C. acaciivora) and the lowest recorded (15.55%; C. manginecans and 21.67%; C. acaciivora) were observed with isolate KHES/AFI-UMS/E001; Verticillium sp. This study showed that isolate Trichoderma sp. has a good antagonistic effect on C.manginecans and C. acaciivora mycelial growth. Diseases developments from C.manginecans and C. acaciivora inoculated samples which suggesting that this fungus is the primary cause of the wilting of trees under natural conditions. 2019 Thesis https://eprints.ums.edu.my/id/eprint/41351/ https://eprints.ums.edu.my/id/eprint/41351/1/ABSTRACT.pdf text en public https://eprints.ums.edu.my/id/eprint/41351/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Faculty Of Science And Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	SB317.5-319.864 Horticulture Horticultural crops
spellingShingle	SB317.5-319.864 Horticulture Horticultural crops Khesnii Shiv Ram Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
description	A radical rise posting in the incidence of wilting and dieback diseases induced by plant pathogenic fungi Ceratocystis sp. in commercially planted Acacia mangium are major problem faced by industrial plantations such as Acacia Forest Industry (AFI) Plantation. Two types of Ceratocystis isolates;Ceratocystis manginecans (AFI/CM/001) and Ceratocystis acaciivora (AFI/CA/001) are recognized and collected from AFI Plantation in Pitas, Sabah. The general aim is to scientifically determine the immensity of Ceratocystis isolates to induce disease on plantation grown A. mangium using pathogenicity and interaction study. On this objective, field inoculation is done in the plantation nursery and interaction behavior is to be observed in-vitro. In field inoculation, 100 A. mangium seedlings were inoculated with C. manginecans and C. acaciivora to observe the ability of both fungi to induce diseases for 6 weeks. The efficacy study shows both fungi have capability to enhance lesion on A. mangium seedlings. C. manginecans isolate produced longer lesions (mean=47.103) compared to C. acaciivora isolate (mean=42.940), the difference is not significant (p=0.148, F=2.099). Yet, C. acaciivora showed a more linear growth in lesion increment compared to C. manginecans by weekly observation. Dual culture method was used to assess the ability of 6 test isolates which were identified during the isolation of infected A. mangium sample to control C. manginecans and C. acaciivora, which causes the wilting and dieback diseases. The highest percentage inhibition of radical growth (PIRG) values was observed with isolate KHES/AFI-UMS/D001; Trichoderma sp (60.00%; C. manginecans and 53.33%; C. acaciivora) and the lowest recorded (15.55%; C. manginecans and 21.67%; C. acaciivora) were observed with isolate KHES/AFI-UMS/E001; Verticillium sp. This study showed that isolate Trichoderma sp. has a good antagonistic effect on C.manginecans and C. acaciivora mycelial growth. Diseases developments from C.manginecans and C. acaciivora inoculated samples which suggesting that this fungus is the primary cause of the wilting of trees under natural conditions.
format	Thesis
qualification_level	Master's degree
author	Khesnii Shiv Ram
author_facet	Khesnii Shiv Ram
author_sort	Khesnii Shiv Ram
title	Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
title_short	Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
title_full	Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
title_fullStr	Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
title_full_unstemmed	Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah
title_sort	pathogenicity study of ceratocystis acaiivora and ceratocystis manginecans associated with wilting & dieback diseases on acacia mangium at acacia forest industry (afi), sabah
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty Of Science And Natural Resources
publishDate	2019
url	https://eprints.ums.edu.my/id/eprint/41351/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41351/2/FULLTEXT.pdf
_version_	1818611394193391616

Pathogenicity study of Ceratocystis acaiivora and Ceratocystis manginecans associated with wilting & dieback diseases on Acacia mangium at Acacia Forest Industry (AFI), Sabah

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