Survival kinetics of lactobacillus species during exposure to simulated gastric fluid

The overall aim of this research was to develop a standard quantitative method to evaluate the acid tolerance of probiotic cells. The approach was to expose the cells of different concentrations (103- 109 CFU/ml) to a range of pH (i.e. pH 1.5 to 2.5) in order to simulate the varying acidity of stoma...

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Main Author: Lee, Peh Phong
Format: Thesis
Language:English
English
Published: 2010
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Online Access:https://eprints.ums.edu.my/id/eprint/41395/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41395/2/FULLTEXT.pdf
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spelling my-ums-ep.413952024-11-01T01:14:23Z Survival kinetics of lactobacillus species during exposure to simulated gastric fluid 2010 Lee, Peh Phong TP200-248 Chemicals: Manufacture, use, etc. The overall aim of this research was to develop a standard quantitative method to evaluate the acid tolerance of probiotic cells. The approach was to expose the cells of different concentrations (103- 109 CFU/ml) to a range of pH (i.e. pH 1.5 to 2.5) in order to simulate the varying acidity of stomach. A standard simulated gastric fluid of fixed volume and three model probiotic cells were used in this study. The cell survival kinetics was determined and was described with a mathematical correlation. It was found that the overall death constant (kd ) for three tested strains increased with decrease in the pH and cell concentration. The death constant could be expressed by a general mathematical correlation, kd = k All[pH-9.02 . N0-0.19] where kAll is the acid intolerance indicator and No is the initial cell concentration. The data fitting analysis of this equation give 0.97701 values for coefficient of determination. The k All values of Lactobacillus acidophilus ATCC4356 was found to be lowest followed by Lactobacillus casei Shirota and Lactobacillus casei 01 was the highest. This indicates decreasing in acid intolerance of the cells. In conclusion, a standard and quantitative method has been developed to measure the acid tolerance of probiotic cells and to facilitate selection of strain and process technology. 2010 Thesis https://eprints.ums.edu.my/id/eprint/41395/ https://eprints.ums.edu.my/id/eprint/41395/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/41395/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Sekolah Kejuruteraan dan Teknologi Maklumat
institution Universiti Malaysia Sabah
collection UMS Institutional Repository
language English
English
topic TP200-248 Chemicals: Manufacture
use
etc.
spellingShingle TP200-248 Chemicals: Manufacture
use
etc.
Lee, Peh Phong
Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
description The overall aim of this research was to develop a standard quantitative method to evaluate the acid tolerance of probiotic cells. The approach was to expose the cells of different concentrations (103- 109 CFU/ml) to a range of pH (i.e. pH 1.5 to 2.5) in order to simulate the varying acidity of stomach. A standard simulated gastric fluid of fixed volume and three model probiotic cells were used in this study. The cell survival kinetics was determined and was described with a mathematical correlation. It was found that the overall death constant (kd ) for three tested strains increased with decrease in the pH and cell concentration. The death constant could be expressed by a general mathematical correlation, kd = k All[pH-9.02 . N0-0.19] where kAll is the acid intolerance indicator and No is the initial cell concentration. The data fitting analysis of this equation give 0.97701 values for coefficient of determination. The k All values of Lactobacillus acidophilus ATCC4356 was found to be lowest followed by Lactobacillus casei Shirota and Lactobacillus casei 01 was the highest. This indicates decreasing in acid intolerance of the cells. In conclusion, a standard and quantitative method has been developed to measure the acid tolerance of probiotic cells and to facilitate selection of strain and process technology.
format Thesis
qualification_level Master's degree
author Lee, Peh Phong
author_facet Lee, Peh Phong
author_sort Lee, Peh Phong
title Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
title_short Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
title_full Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
title_fullStr Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
title_full_unstemmed Survival kinetics of lactobacillus species during exposure to simulated gastric fluid
title_sort survival kinetics of lactobacillus species during exposure to simulated gastric fluid
granting_institution Universiti Malaysia Sabah
granting_department Sekolah Kejuruteraan dan Teknologi Maklumat
publishDate 2010
url https://eprints.ums.edu.my/id/eprint/41395/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41395/2/FULLTEXT.pdf
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