Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita

Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloi...

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Main Author:	Fatin Ruzanna Jamaluddin
Format:	Thesis
Language:	English English
Published:	2013
Subjects:	QK710-899 Plant physiology
Online Access:	https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf
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spelling	my-ums-ep.417912024-11-29T04:53:38Z Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita 2013 Fatin Ruzanna Jamaluddin QK710-899 Plant physiology Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloids, phytosterols, saponins and phenolic compounds were present in the sapwood, heartwood and bark, whereas only phytosterols were present in hexane extracts. Therefore, determination of antioxidant and antimicrobial activities were carried out only for the methanolic crude extracts of wood and bark. The methanolic crude extracts were tested for primary and secondary antioxidant properties by measuring the total phenolic contents (TPC) and the 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The extracts demonstrated higher TPCs and free radical scavenging activities against DPPH, especially for the bark and most parts of young wood as compared to matured wood of N. subdita . Study was also carried out to test antimicrobial activities of the methanolic crude extracts against four bacteria, i.e. Bacillus subtilis, Vibrio parahaemoliticus, Vibrio alginolyticus, Bukholderia cepacia, and two fungi, i.e. Candida albican and Aspergillus niger . The results indicated that all the samples have moderate to good zones of inhibition on the bacteria growth when compared to the positive control. However, there was no zone of inhibition against the two fungi (A. niger and C. albican ) used. Gas chromatography-mass spectrometry (GC-MS) technique was also employed for the analysis of semi-volatile compounds in six selected crude extracts from young and matured woods of N. subdita . The groups of compound were identified are from hydrocarbon, alcohol, ester, phenol, ether, ketone, organofluorine, organoclorine and organic acid groups. 2013 Thesis https://eprints.ums.edu.my/id/eprint/41791/ https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Sekolah Perhutanan Tropika Antarabangsa
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK710-899 Plant physiology
spellingShingle	QK710-899 Plant physiology Fatin Ruzanna Jamaluddin Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
description	Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloids, phytosterols, saponins and phenolic compounds were present in the sapwood, heartwood and bark, whereas only phytosterols were present in hexane extracts. Therefore, determination of antioxidant and antimicrobial activities were carried out only for the methanolic crude extracts of wood and bark. The methanolic crude extracts were tested for primary and secondary antioxidant properties by measuring the total phenolic contents (TPC) and the 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The extracts demonstrated higher TPCs and free radical scavenging activities against DPPH, especially for the bark and most parts of young wood as compared to matured wood of N. subdita . Study was also carried out to test antimicrobial activities of the methanolic crude extracts against four bacteria, i.e. Bacillus subtilis, Vibrio parahaemoliticus, Vibrio alginolyticus, Bukholderia cepacia, and two fungi, i.e. Candida albican and Aspergillus niger . The results indicated that all the samples have moderate to good zones of inhibition on the bacteria growth when compared to the positive control. However, there was no zone of inhibition against the two fungi (A. niger and C. albican ) used. Gas chromatography-mass spectrometry (GC-MS) technique was also employed for the analysis of semi-volatile compounds in six selected crude extracts from young and matured woods of N. subdita . The groups of compound were identified are from hydrocarbon, alcohol, ester, phenol, ether, ketone, organofluorine, organoclorine and organic acid groups.
format	Thesis
qualification_level	Master's degree
author	Fatin Ruzanna Jamaluddin
author_facet	Fatin Ruzanna Jamaluddin
author_sort	Fatin Ruzanna Jamaluddin
title	Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_short	Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_full	Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_fullStr	Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_full_unstemmed	Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_sort	antioxidant and antimicrobial properties of chemical extractives from nauclea subdita
granting_institution	Universiti Malaysia Sabah
granting_department	Sekolah Perhutanan Tropika Antarabangsa
publishDate	2013
url	https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf
_version_	1818611429363679232

Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita

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