Investigation on protein extraction from algae using low voltage pulsed electric field
Extraction of specific biological components like protein from microalgae is often prevented by the intrinsic rigidity of the cell wall. Therefore, cell wall disruption is required to permit access to the internal components of the cells. The purpose of this study is to extract protein from microalg...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/42024/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/42024/2/FULLTEXT.pdf |
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| Summary: | Extraction of specific biological components like protein from microalgae is often prevented by the intrinsic rigidity of the cell wall. Therefore, cell wall disruption is required to permit access to the internal components of the cells. The purpose of this study is to extract protein from microalgae using low voltage Pulsed Electric Field (PEF). Microalgae Chlorella vulgaris, Scenedesmus quadricauda and Chlorococcum sp. were treated with PEF with electrical field of 380 V/cm in silver (Ag) and stainless steel (SST) parallel plate treatment chambers for 30 minutes. The treated microalgae samples then went through solid-liquid separation by centrifugation. The total protein extracted was quantified using quantitative Bradford Assay. Results showed that overheating occurred at the electrode's negative terminal during the square pulse PEF treatments, causing damages to the cells due to long pulse duration with 60% duty cycle. Lowering the duty cycle or shortening the pulse duration of the PEF pulse reduced the overheating effect during the treatment. This is achieved using exponential decay PEF pulse that enabled longer treatment time up to 30 minutes while minimizing the cell damages. For Chlorococcum sp., PEF treatment with electrical field strength 190 V/cm and duty cycle 8% produced higher protein concentration than the other set parameters. Meanwhile, C. vulgaris and S. quadricauda required longer pulse duration with duty cycle 16% with electrical field strength 190 V/cm or lower duty cycle 8% and electrical filed strength 380 V/cm to allow a higher concentration of extracted protein. All the treated microalgae showed positive viability as they were grown after the treatment. Overall, different cell morphology of the microalgae required particular pulse parameters to ensure successful protein extraction. |
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