Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo

Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo

Boesenbergia, also known as Kaempferia is one of the genus from the family of Zingiberaceae. The species of Zingiberaceae family have been reported to possess both antioxidant and anti-inflammatory activity, and thus might be effective as anticancer agents. Boesenbergia has many species that are lo...

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Main Author: Ling, Jing Jing
Format: Thesis
Language:English
English
Published: 2010
Subjects:
QK495 Angiosperms
Online Access:https://eprints.ums.edu.my/id/eprint/9581/7/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/9581/8/FULLTEXT.pdf
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spelling my-ums-ep.95812024-04-23T06:46:24Z Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo 2010 Ling, Jing Jing QK495 Angiosperms Boesenbergia, also known as Kaempferia is one of the genus from the family of Zingiberaceae. The species of Zingiberaceae family have been reported to possess both antioxidant and anti-inflammatory activity, and thus might be effective as anticancer agents. Boesenbergia has many species that are locally used in the preparation of tonic, 'jamu' and traditional medicine. The aims of the present study started by discovering the ethnobotany of Boesenbergia species from recorded data by local people in Sabah; the selected plants were screened for the antioxidant, total phenolic and flavonoid content as well as cytotoxicity activities; further determination of the phytochemicals (especially flavonoid) were conducted using High Performance Liquid Chromatography (HPLC) for the possible active compounds contributing to antioxidant and anticancer activities. In this study, methanol crude extracts were used. Four different parameters of antioxidant assessments were conducted: DPPH free radical scavenging activity, ABTS decolorization assay, FRAP assay and p-Carotene linoleate bleaching assay. For cytotoxicity activity determination, the plant extracts were screened by using MTT ([3-( 4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide] assay and further evaluated for cell cycle analysis by using flow cytometry. MCF-7 (hormone dependent breast cancer), MDA-MB-231 (non-hormone dependent breast cancer), CaOV3 (ovarian cancer), HeLa (cervical cancer) and HT-29 (colon cancer) were cultured for cytotoxicity activity. In this study, the leaves and rhizomes from B. rotunda (L.) Mansf. in Kulturpfl., B. pulchella (Ridley) Merrill, B. pulchella var attenuata R. M. Smith, B. sp 1 and B. armeniaca Cowley were analysed separately. B. rotunda was used as a positive control as it has anticancer activity towards certain cancer cell lines such as HT29 colon cancer cells and MCF-7 breasts cancer cells. In general, B. pulchella and 8. pulchella var attenuata displayed better antioxidant activity compared to the positive control. For MCF-7, B. rotunda (rhizome), B. pulchella var attenuata (rhizome) and B. armeniaca (rhizome) displayed positive cytotoxic effects with IC50 values of 51, 93±2.83 and 94.5±0.71 µg/ml, respectively. Naringin, hesperidin, quercetin and luteolin were detected as the major active compounds that might be contributed to antioxidant and anticancer activities. Thus, the Boesenbergia species collected from Sabah rainforest especially B. pulchella var attenuata (rhizome) and B. armeniaca (rhizome) would be promising anticancer remedy for hormone dependent breast cancer. 2010 Thesis https://eprints.ums.edu.my/id/eprint/9581/ https://eprints.ums.edu.my/id/eprint/9581/7/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/9581/8/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Biologi Tropika dan Pemuliharaan
institution Universiti Malaysia Sabah
collection UMS Institutional Repository
language English
English
topic QK495 Angiosperms
spellingShingle QK495 Angiosperms
Ling, Jing Jing
Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
description Boesenbergia, also known as Kaempferia is one of the genus from the family of Zingiberaceae. The species of Zingiberaceae family have been reported to possess both antioxidant and anti-inflammatory activity, and thus might be effective as anticancer agents. Boesenbergia has many species that are locally used in the preparation of tonic, 'jamu' and traditional medicine. The aims of the present study started by discovering the ethnobotany of Boesenbergia species from recorded data by local people in Sabah; the selected plants were screened for the antioxidant, total phenolic and flavonoid content as well as cytotoxicity activities; further determination of the phytochemicals (especially flavonoid) were conducted using High Performance Liquid Chromatography (HPLC) for the possible active compounds contributing to antioxidant and anticancer activities. In this study, methanol crude extracts were used. Four different parameters of antioxidant assessments were conducted: DPPH free radical scavenging activity, ABTS decolorization assay, FRAP assay and p-Carotene linoleate bleaching assay. For cytotoxicity activity determination, the plant extracts were screened by using MTT ([3-( 4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide] assay and further evaluated for cell cycle analysis by using flow cytometry. MCF-7 (hormone dependent breast cancer), MDA-MB-231 (non-hormone dependent breast cancer), CaOV3 (ovarian cancer), HeLa (cervical cancer) and HT-29 (colon cancer) were cultured for cytotoxicity activity. In this study, the leaves and rhizomes from B. rotunda (L.) Mansf. in Kulturpfl., B. pulchella (Ridley) Merrill, B. pulchella var attenuata R. M. Smith, B. sp 1 and B. armeniaca Cowley were analysed separately. B. rotunda was used as a positive control as it has anticancer activity towards certain cancer cell lines such as HT29 colon cancer cells and MCF-7 breasts cancer cells. In general, B. pulchella and 8. pulchella var attenuata displayed better antioxidant activity compared to the positive control. For MCF-7, B. rotunda (rhizome), B. pulchella var attenuata (rhizome) and B. armeniaca (rhizome) displayed positive cytotoxic effects with IC50 values of 51, 93±2.83 and 94.5±0.71 µg/ml, respectively. Naringin, hesperidin, quercetin and luteolin were detected as the major active compounds that might be contributed to antioxidant and anticancer activities. Thus, the Boesenbergia species collected from Sabah rainforest especially B. pulchella var attenuata (rhizome) and B. armeniaca (rhizome) would be promising anticancer remedy for hormone dependent breast cancer.
format Thesis
qualification_level Master's degree
author Ling, Jing Jing
author_facet Ling, Jing Jing
author_sort Ling, Jing Jing
title Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
title_short Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
title_full Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
title_fullStr Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
title_full_unstemmed Phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to Borneo
title_sort phytochemicals, antioxidant and antiproliferative properties of selected boesenbergia species (zingiberaceae) endemic to borneo
granting_institution Universiti Malaysia Sabah
granting_department Institut Biologi Tropika dan Pemuliharaan
publishDate 2010
url https://eprints.ums.edu.my/id/eprint/9581/7/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/9581/8/FULLTEXT.pdf
_version_ 1804890334627364864

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