IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT

IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT

Inflammation has become the focus of global scientific research because of its implication in virtually all human and animal diseases. The anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAID) have their side effects. Therefore, the anti-inflammatory drugs...

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Main Author: NOOR SURYANI MUSA
Format: Thesis
Language:English
Online Access:http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/1/Abstract.pdf
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/2/Full%20Thesis%20-%20NOOR%20SURYANI%20MUSA_GSK2772.pdf
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spelling my-umt-ir.-160212022-01-19T08:16:01Z IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT NOOR SURYANI MUSA Inflammation has become the focus of global scientific research because of its implication in virtually all human and animal diseases. The anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAID) have their side effects. Therefore, the anti-inflammatory drugs from the natural product had been researched in this study. This study aimed to profile and determines the chemical constituents of H. tiliaceus leaves extract and fractions as well to determine the anti-inflammatory activities of methanol extract of Hibiscus tiliaceus leaves (HM) in vitro on lipopolysaccharide (LPS) stimulated RAW264.7 cell lines and in vivo using formalin-induced paw eodema. HM was obtained by extraction process. Further fractionation of HM was done by using solvent-solvent partitioning and yielded four solvent fractions; hexane fraction (HH), dichloromethane fraction (HD), ethyl acetate fraction (HE) and water fraction (HW) of Hibiscus tiliaceus leaves. Antioxidant potency of HM and H. tiliaceus leaves fractions was tested by DPPH bio-autographic assay using thin layer chromatography (TLC) plate and 1, 1- iv diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging at different concentrations ranging from 0.2 to 10 mg/ml. The antibacterial activity was determined using disc diffusion method against Gram-positive and negative bacteria. The inhibition zones of HM and H. tiliaceus leaves fractions were measured. UNIVERSITI MALAYSIA TERENGGANU 2020 Thesis en http://umt-ir.umt.edu.my:8080/handle/123456789/16021 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/1/Abstract.pdf 10686c44ba204e16fe7ca6fdf21a8d96 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/2/Full%20Thesis%20-%20NOOR%20SURYANI%20MUSA_GSK2772.pdf c6f7589c30c33d058b83110343d90a6d http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33
institution Universiti Malaysia Terengganu
collection UMT Repository System
language English
description Inflammation has become the focus of global scientific research because of its implication in virtually all human and animal diseases. The anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAID) have their side effects. Therefore, the anti-inflammatory drugs from the natural product had been researched in this study. This study aimed to profile and determines the chemical constituents of H. tiliaceus leaves extract and fractions as well to determine the anti-inflammatory activities of methanol extract of Hibiscus tiliaceus leaves (HM) in vitro on lipopolysaccharide (LPS) stimulated RAW264.7 cell lines and in vivo using formalin-induced paw eodema. HM was obtained by extraction process. Further fractionation of HM was done by using solvent-solvent partitioning and yielded four solvent fractions; hexane fraction (HH), dichloromethane fraction (HD), ethyl acetate fraction (HE) and water fraction (HW) of Hibiscus tiliaceus leaves. Antioxidant potency of HM and H. tiliaceus leaves fractions was tested by DPPH bio-autographic assay using thin layer chromatography (TLC) plate and 1, 1- iv diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging at different concentrations ranging from 0.2 to 10 mg/ml. The antibacterial activity was determined using disc diffusion method against Gram-positive and negative bacteria. The inhibition zones of HM and H. tiliaceus leaves fractions were measured.
format Thesis
author NOOR SURYANI MUSA
spellingShingle NOOR SURYANI MUSA
IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
author_facet NOOR SURYANI MUSA
author_sort NOOR SURYANI MUSA
title IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
title_short IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
title_full IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
title_fullStr IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
title_full_unstemmed IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT
title_sort in vitro and in vivo anti-inflammatory activities of hibiscus tiliaceus leaves extract
granting_institution UNIVERSITI MALAYSIA TERENGGANU
url http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/1/Abstract.pdf
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16021/2/Full%20Thesis%20-%20NOOR%20SURYANI%20MUSA_GSK2772.pdf
_version_ 1747835774526554112

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