Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoc...
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my-umt-ir.-19672012-10-08T03:43:16Z Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) Willy, Yee An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection. Terengganu : Universiti Malaysia Terengganu 2011-02 Thesis en http://hdl.handle.net/123456789/1967 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/1/QR%2082%20.R45%20W5%202011%20Abstract.pdf 90ab146b31083617f553e36b825257f1 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/2/QR%2082%20.R45%20W5%202011%20FullText.pdf a977689ce2de306baadc22bd02f3f074 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 QR 82 .R45 W5 2011 Willy, Yee Tesis FST 2011 Agrobacterium |
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Universiti Malaysia Terengganu |
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English |
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QR 82 .R45 W5 2011 QR 82 .R45 W5 2011 Tesis FST 2011 Agrobacterium |
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QR 82 .R45 W5 2011 QR 82 .R45 W5 2011 Tesis FST 2011 Agrobacterium Willy, Yee Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
description |
An important step in the development of an efficient Agrobacterium-medialed
transformation system is the establishment of optimal conditions for efficient TDNA
transfer. Parameters known to influence the efficacy of Agrobacterium
tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation
temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS
expression two days post-infection. |
format |
Thesis |
author |
Willy, Yee |
author_facet |
Willy, Yee |
author_sort |
Willy, Yee |
title |
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
title_short |
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
title_full |
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
title_fullStr |
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
title_full_unstemmed |
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) |
title_sort |
development of agrobacterium-mediated transformation method for marine microalgae (chlorella sp. and nannochloropsis sp.) |
granting_institution |
Terengganu : Universiti Malaysia Terengganu |
url |
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/1/QR%2082%20.R45%20W5%202011%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/2/QR%2082%20.R45%20W5%202011%20FullText.pdf |
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