Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants
This study characterised 7 isolates of Edwardsiella tarda from African catfish, Clarias gariepinus. The isolated E. tarda were identified using a combination of 29 conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done o...
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my-umt-ir.-8022016-03-11T20:40:36Z Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Lee Seong Wei This study characterised 7 isolates of Edwardsiella tarda from African catfish, Clarias gariepinus. The isolated E. tarda were identified using a combination of 29 conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done on these bacterial isolates using three universal primers, namely WTP, (GTG)s and M13 universal. Characterisation of whole cell protein profiles of the isolates were also carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the present study, minimum inhibitory concentration (MIC) values of 20 edible plant extracts against E. tarda were determined through a two fold microdilution technique. Fakulti Agroteknologi dan Sains Makanan 2009-05 Thesis en http://hdl.handle.net/123456789/802 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/3/license.txt ecca7a0b60eabbf88d99eae3662e2d50 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/4/QR%2082%20.E6%20L4%202009%20Abstract.pdf e24b82a78afaf8c273cd83ddd0a56297 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/5/QR%2082%20.E6%20L4%202009%20FullText.pdf 075cd8020f59ec4fd53f7f18101029bd http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/6/QR%2082%20.E6%20L4%202009%20Abstract.pdf.txt 21e8793bf2fc087aa0eb100fb908fa1e http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/7/QR%2082%20.E6%20L4%202009%20FullText.pdf.txt da7a03ee3c3f5919d7acec0b3c89a917 QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda |
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QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda |
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QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
description |
This study characterised 7 isolates of Edwardsiella tarda from African catfish,
Clarias gariepinus. The isolated E. tarda were identified using a combination of 29
conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done on these bacterial isolates
using three universal primers, namely WTP, (GTG)s and M13 universal. Characterisation of whole cell protein profiles of the isolates were also carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the present study, minimum inhibitory concentration (MIC) values of 20 edible plant extracts against E. tarda were determined through a two fold microdilution technique. |
format |
Thesis |
author |
Lee Seong Wei |
author_facet |
Lee Seong Wei |
author_sort |
Lee Seong Wei |
title |
Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
title_short |
Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
title_full |
Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
title_fullStr |
Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
title_full_unstemmed |
Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
title_sort |
characteristation of edwardsiella tarda isolated from local calrias gariepinus and potential remedies from edible plants |
granting_institution |
Fakulti Agroteknologi dan Sains Makanan |
url |
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/4/QR%2082%20.E6%20L4%202009%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/802/5/QR%2082%20.E6%20L4%202009%20FullText.pdf |
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