Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been st...
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| Format: | Thesis |
| Language: | English |
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| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/2/Full%20text.pdf |
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| Summary: | The potential of C. grandis tissue in producing high amount of ascorbic acid has been
investigated in this study. In tissue culture technique, nutrients in the media have been a
major factor in manipulating the final yield required. Phosphate, nitrate and sucrose
with incubation time have been studied on their influence in production of ascorbic acid
in C. grandis suspension cultures. The result obtained in factorial design indicates that
among all four factors, only sucrose and incubation time give significant effect when
present alone. However, all four factors have influence in the production when present
together. This result was verified by the significant interaction among all the four
factors. Then, suitable ranges of concentration for selected factors that obtained from
factorial design were used in the optimization study. By using response surface
methodology (RSM) through central composite design (CCD), the optimum conditions
of selected factors were obtained. The result shows that when callus was supplied with
506.3mg/l phosphate, 1916.7 mg/l nitrate and 65.2 g/l sucrose and incubated in dark
room for 7 days, maximum production of ascorbic acid at 34.53 ± 2.20 mg/l was
obtained. The results obtained were in the range of predicted ascorbic acid and therefore
the model of the study is acceptable. Then, the cultivation of callus in a bioreactor was
carried out. The concentration of ascorbic acid obtained was 9 mg/l of which is very low
compared to the production obtained in flask. This is due to the restriction provided by
the unsuitable condition in the bioreactor for the growth of callus. |
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