Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been st...
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my-unimap-407172015-12-31T13:34:47Z Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck Nor Alina, Nazri The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been studied on their influence in production of ascorbic acid in C. grandis suspension cultures. The result obtained in factorial design indicates that among all four factors, only sucrose and incubation time give significant effect when present alone. However, all four factors have influence in the production when present together. This result was verified by the significant interaction among all the four factors. Then, suitable ranges of concentration for selected factors that obtained from factorial design were used in the optimization study. By using response surface methodology (RSM) through central composite design (CCD), the optimum conditions of selected factors were obtained. The result shows that when callus was supplied with 506.3mg/l phosphate, 1916.7 mg/l nitrate and 65.2 g/l sucrose and incubated in dark room for 7 days, maximum production of ascorbic acid at 34.53 ± 2.20 mg/l was obtained. The results obtained were in the range of predicted ascorbic acid and therefore the model of the study is acceptable. Then, the cultivation of callus in a bioreactor was carried out. The concentration of ascorbic acid obtained was 9 mg/l of which is very low compared to the production obtained in flask. This is due to the restriction provided by the unsuitable condition in the bioreactor for the growth of callus. Universiti Malaysia Perlis (UniMAP) 2010 Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/40717 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/1/Page%201-24.pdf 7cfc7df6d473886120764259a48d2190 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/2/Full%20text.pdf add096a2454d7ecd945f6632a976034e http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Ascorbic acid Citrus grandis Phosphate Nitrate Sucrose School of Bioprocess Engineering |
| institution |
Universiti Malaysia Perlis |
| collection |
UniMAP Institutional Repository |
| language |
English |
| topic |
Ascorbic acid Citrus grandis Phosphate Nitrate Sucrose |
| spellingShingle |
Ascorbic acid Citrus grandis Phosphate Nitrate Sucrose Nor Alina, Nazri Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| description |
The potential of C. grandis tissue in producing high amount of ascorbic acid has been
investigated in this study. In tissue culture technique, nutrients in the media have been a
major factor in manipulating the final yield required. Phosphate, nitrate and sucrose
with incubation time have been studied on their influence in production of ascorbic acid
in C. grandis suspension cultures. The result obtained in factorial design indicates that
among all four factors, only sucrose and incubation time give significant effect when
present alone. However, all four factors have influence in the production when present
together. This result was verified by the significant interaction among all the four
factors. Then, suitable ranges of concentration for selected factors that obtained from
factorial design were used in the optimization study. By using response surface
methodology (RSM) through central composite design (CCD), the optimum conditions
of selected factors were obtained. The result shows that when callus was supplied with
506.3mg/l phosphate, 1916.7 mg/l nitrate and 65.2 g/l sucrose and incubated in dark
room for 7 days, maximum production of ascorbic acid at 34.53 ± 2.20 mg/l was
obtained. The results obtained were in the range of predicted ascorbic acid and therefore
the model of the study is acceptable. Then, the cultivation of callus in a bioreactor was
carried out. The concentration of ascorbic acid obtained was 9 mg/l of which is very low
compared to the production obtained in flask. This is due to the restriction provided by
the unsuitable condition in the bioreactor for the growth of callus. |
| format |
Thesis |
| author |
Nor Alina, Nazri |
| author_facet |
Nor Alina, Nazri |
| author_sort |
Nor Alina, Nazri |
| title |
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| title_short |
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| title_full |
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| title_fullStr |
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| title_full_unstemmed |
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck |
| title_sort |
optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of citrus grandis (l.) osbeck |
| granting_institution |
Universiti Malaysia Perlis (UniMAP) |
| granting_department |
School of Bioprocess Engineering |
| url |
http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/40717/2/Full%20text.pdf |
| _version_ |
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