The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization

This thesis discussed on the optimization of MWCNTs surface modification via nitric acid oxidation for DNA immobilization. After acid oxidation treatment, the impurities in multi-walled carbon nanotube (MWCNTs) such as carbonaceous and metal catalyst particles are successfully reduced as has been an...

Full description

Saved in:

Bibliographic Details
Main Author:	Shahidah Arina, Shamsuddin
Format:	Thesis
Language:	English
Subjects:	DNA immobilization Nitric acid oxidation Multi-walled carbon nanotube (MWCNTs) Carbon nanotubes DNA biosensor application
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/1/p.1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/2/Full%20text.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimap-44113
record_format	uketd_dc
spelling	my-unimap-441132016-11-22T04:42:55Z The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization Shahidah Arina, Shamsuddin This thesis discussed on the optimization of MWCNTs surface modification via nitric acid oxidation for DNA immobilization. After acid oxidation treatment, the impurities in multi-walled carbon nanotube (MWCNTs) such as carbonaceous and metal catalyst particles are successfully reduced as has been analyzed by energy dispersive x-ray spectroscopy (EDS), x-ray diffraction (XRD) and thermogravimetric analyzer (TGA). Acid oxidation will caused to the opening of MWCNTs tips and structural defects formed on the MWCNTs surface due to the acid attack. Oxygen containing functional groups, mainly, carboxylic group (COOH) has been introduced on the MWCNTs opened tips and at the defect sites which are useful to interact with other molecules, in this case, aminated-ssDNA probe. The results from fourier transform infrared spectroscopy (FTIR) and Raman Spectroscopy have shown that the COOH amount is depended on the MWCNTs structure defects. Meanwhile, cyclic voltammetry (CV) results have indicated that the immobilization current is directly proportional to the COOH amount. However, structure defect will affect to the immobilization current when ID/IG ratio is increased. The acid oxidation parameter should be optimized, thus the amount of COOH can be increased with the minimal structure defect. Therefore, the main goal to have a maximum immobilization current can be achieved. L9 Taguchi orthogonal array has been used to optimize the acid oxidation parameters. From the result, 5 M of nitric acid concentration, 120 °C of treatment temperature and 6 hours of treatment time are selected as the most optimum combination of acid oxidation parameters. The percentage influence of each main factor is also calculated to be 46% xvi 35% and 18% for nitric acid concentration, treatment time and treatment temperature, respectively. The improvement is happened to be 11.6% of increment in the immobilization current. Universiti Malaysia Perlis (UniMAP) 2014 Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/44113 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/1/p.1-24.pdf c2b9748c7b6655f4c5ef0f54a3de8321 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/2/Full%20text.pdf cc6e4214e228d454c1f5e887c27e8b99 DNA immobilization Nitric acid oxidation Multi-walled carbon nanotube (MWCNTs) Carbon nanotubes DNA biosensor application Institute of Nano Electronic Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
topic	DNA immobilization Nitric acid oxidation Multi-walled carbon nanotube (MWCNTs) Carbon nanotubes DNA biosensor application
spellingShingle	DNA immobilization Nitric acid oxidation Multi-walled carbon nanotube (MWCNTs) Carbon nanotubes DNA biosensor application Shahidah Arina, Shamsuddin The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
description	This thesis discussed on the optimization of MWCNTs surface modification via nitric acid oxidation for DNA immobilization. After acid oxidation treatment, the impurities in multi-walled carbon nanotube (MWCNTs) such as carbonaceous and metal catalyst particles are successfully reduced as has been analyzed by energy dispersive x-ray spectroscopy (EDS), x-ray diffraction (XRD) and thermogravimetric analyzer (TGA). Acid oxidation will caused to the opening of MWCNTs tips and structural defects formed on the MWCNTs surface due to the acid attack. Oxygen containing functional groups, mainly, carboxylic group (COOH) has been introduced on the MWCNTs opened tips and at the defect sites which are useful to interact with other molecules, in this case, aminated-ssDNA probe. The results from fourier transform infrared spectroscopy (FTIR) and Raman Spectroscopy have shown that the COOH amount is depended on the MWCNTs structure defects. Meanwhile, cyclic voltammetry (CV) results have indicated that the immobilization current is directly proportional to the COOH amount. However, structure defect will affect to the immobilization current when ID/IG ratio is increased. The acid oxidation parameter should be optimized, thus the amount of COOH can be increased with the minimal structure defect. Therefore, the main goal to have a maximum immobilization current can be achieved. L9 Taguchi orthogonal array has been used to optimize the acid oxidation parameters. From the result, 5 M of nitric acid concentration, 120 °C of treatment temperature and 6 hours of treatment time are selected as the most optimum combination of acid oxidation parameters. The percentage influence of each main factor is also calculated to be 46% xvi 35% and 18% for nitric acid concentration, treatment time and treatment temperature, respectively. The improvement is happened to be 11.6% of increment in the immobilization current.
format	Thesis
author	Shahidah Arina, Shamsuddin
author_facet	Shahidah Arina, Shamsuddin
author_sort	Shahidah Arina, Shamsuddin
title	The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
title_short	The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
title_full	The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
title_fullStr	The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
title_full_unstemmed	The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
title_sort	optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	Institute of Nano Electronic Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/1/p.1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/44113/2/Full%20text.pdf
_version_	1747836822228041728

The optimization of multi-walled carbon nanotubes surface modification via nitric acid oxidation for dna immobilization

Similar Items