Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection

Breakthrough in nanotechnology provides a great extent in biosensor development and application. Previous studies showed that nanogap sensor device provides excellent electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having a pair of electrodes facing each other, whi...

Full description

Saved in:

Bibliographic Details
Format:	Thesis
Language:	English
Subjects:	Nanotechnology Biosensors Escherichia coli
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/3/Declaration%20Form.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimap-76733
record_format	uketd_dc
spelling	my-unimap-767332022-11-08T02:55:57Z Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection Uda, Hashim, Prof. Dr. Breakthrough in nanotechnology provides a great extent in biosensor development and application. Previous studies showed that nanogap sensor device provides excellent electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having a pair of electrodes facing each other, which a molecule trapped in between its will be identified by observing the electrical characterization. Conventional development process requires prolonged and tedious compulsory additional method. Thus this research project focus on developing various size of uniform nanogap structure in nanometre scales which are capable of sensing Escherichia coli O157:H7 (E. coli O157:H7) at a low concentration level. The development of the device was divided into nanogap structure and gold pad structure process using electron beam lithography (EBL) method and conventional photolithography method respectively. Silicon on insulator (SOI) substrate was used to fabricate the nanogap structure and gold was used as a gold pad for a probing purpose. The developed nanogap devices was physically characterized by Field Emission Scanning Electron Microscopy and Scanning Electron Microscope. Meanwhile, the performance of the devices was tested by evaluating the capacitance and impedance reading by sweeping a frequency from 1M Hz to 0.1 Hz at room temperature with 1.0 mV input using Dielectric Analyzer. The devices were tested with de-ionized water and different pH level to optimize the sensor sensitivity that related to the nanogap size. Prior to the detection of E. coli deoxyribonucleic acid (DNA), the device was surface modified with NH2-Amine functionalized silane group using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde for DNA to be covalently bonded with the APTES modified surface. The principle of the E. coli detection is based on charge density changes of the DNA probe immobilization and DNA target hybridization on the modified surface. The morphological testing results shows that the developed devices were observed with 40, 80 and 100 nm nanogap size. It was found that, the device with smallest gap size, 40 nm shows the highest sensitivity and stability compared to the device with bigger gap size, 80 and 100 nm. In this project 40 nm size nanogap device was successfully developed as biosensor for E. coli O157: H7 detection with capability to distinguish the impedance value between complementary, non-complementary and single mismatch DNA sequences. In addition, the device was able to detect E. coli O157: H7 DNA target at concentration limit from 10 nM to 1 pM with linear regression equation is ( ) = 3 × 10−7 + 5 × 10−9 and the correlation coefficient is 0.98. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/76733 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/4/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/1/Page%201-24.pdf bfd46ac49028bcb3afa6567adc4b2ee3 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/2/Full%20text.pdf d687872dc523e489990dc3b887868c10 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/3/Declaration%20Form.pdf 84e754cbf2e1fb9fe8ad9cadd7b43a61 Universiti Malaysia Perlis (UniMAP) Nanotechnology Biosensors Escherichia coli Institute of Nano Electronic Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Uda, Hashim, Prof. Dr.
topic	Nanotechnology Biosensors Escherichia coli
spellingShingle	Nanotechnology Biosensors Escherichia coli Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
description	Breakthrough in nanotechnology provides a great extent in biosensor development and application. Previous studies showed that nanogap sensor device provides excellent electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having a pair of electrodes facing each other, which a molecule trapped in between its will be identified by observing the electrical characterization. Conventional development process requires prolonged and tedious compulsory additional method. Thus this research project focus on developing various size of uniform nanogap structure in nanometre scales which are capable of sensing Escherichia coli O157:H7 (E. coli O157:H7) at a low concentration level. The development of the device was divided into nanogap structure and gold pad structure process using electron beam lithography (EBL) method and conventional photolithography method respectively. Silicon on insulator (SOI) substrate was used to fabricate the nanogap structure and gold was used as a gold pad for a probing purpose. The developed nanogap devices was physically characterized by Field Emission Scanning Electron Microscopy and Scanning Electron Microscope. Meanwhile, the performance of the devices was tested by evaluating the capacitance and impedance reading by sweeping a frequency from 1M Hz to 0.1 Hz at room temperature with 1.0 mV input using Dielectric Analyzer. The devices were tested with de-ionized water and different pH level to optimize the sensor sensitivity that related to the nanogap size. Prior to the detection of E. coli deoxyribonucleic acid (DNA), the device was surface modified with NH2-Amine functionalized silane group using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde for DNA to be covalently bonded with the APTES modified surface. The principle of the E. coli detection is based on charge density changes of the DNA probe immobilization and DNA target hybridization on the modified surface. The morphological testing results shows that the developed devices were observed with 40, 80 and 100 nm nanogap size. It was found that, the device with smallest gap size, 40 nm shows the highest sensitivity and stability compared to the device with bigger gap size, 80 and 100 nm. In this project 40 nm size nanogap device was successfully developed as biosensor for E. coli O157: H7 detection with capability to distinguish the impedance value between complementary, non-complementary and single mismatch DNA sequences. In addition, the device was able to detect E. coli O157: H7 DNA target at concentration limit from 10 nM to 1 pM with linear regression equation is ( ) = 3 × 10−7 + 5 × 10−9 and the correlation coefficient is 0.98.
format	Thesis
title	Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
title_short	Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
title_full	Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
title_fullStr	Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
title_full_unstemmed	Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
title_sort	development of silicon on insulator based nanogap sensor for escherichia coli o157:h7 detection
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	Institute of Nano Electronic Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/3/Declaration%20Form.pdf
_version_	1776104273448271872

Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection

Similar Items