Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection
Breakthrough in nanotechnology provides a great extent in biosensor development and application. Previous studies showed that nanogap sensor device provides excellent electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having a pair of electrodes facing each other, whi...
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my-unimap-767332022-11-08T02:55:57Z Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection Uda, Hashim, Prof. Dr. Breakthrough in nanotechnology provides a great extent in biosensor development and application. Previous studies showed that nanogap sensor device provides excellent electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having a pair of electrodes facing each other, which a molecule trapped in between its will be identified by observing the electrical characterization. Conventional development process requires prolonged and tedious compulsory additional method. Thus this research project focus on developing various size of uniform nanogap structure in nanometre scales which are capable of sensing Escherichia coli O157:H7 (E. coli O157:H7) at a low concentration level. The development of the device was divided into nanogap structure and gold pad structure process using electron beam lithography (EBL) method and conventional photolithography method respectively. Silicon on insulator (SOI) substrate was used to fabricate the nanogap structure and gold was used as a gold pad for a probing purpose. The developed nanogap devices was physically characterized by Field Emission Scanning Electron Microscopy and Scanning Electron Microscope. Meanwhile, the performance of the devices was tested by evaluating the capacitance and impedance reading by sweeping a frequency from 1M Hz to 0.1 Hz at room temperature with 1.0 mV input using Dielectric Analyzer. The devices were tested with de-ionized water and different pH level to optimize the sensor sensitivity that related to the nanogap size. Prior to the detection of E. coli deoxyribonucleic acid (DNA), the device was surface modified with NH2-Amine functionalized silane group using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde for DNA to be covalently bonded with the APTES modified surface. The principle of the E. coli detection is based on charge density changes of the DNA probe immobilization and DNA target hybridization on the modified surface. The morphological testing results shows that the developed devices were observed with 40, 80 and 100 nm nanogap size. It was found that, the device with smallest gap size, 40 nm shows the highest sensitivity and stability compared to the device with bigger gap size, 80 and 100 nm. In this project 40 nm size nanogap device was successfully developed as biosensor for E. coli O157: H7 detection with capability to distinguish the impedance value between complementary, non-complementary and single mismatch DNA sequences. In addition, the device was able to detect E. coli O157: H7 DNA target at concentration limit from 10 nM to 1 pM with linear regression equation is ( ) = 3 × 10−7 + 5 × 10−9 and the correlation coefficient is 0.98. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/76733 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/4/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/1/Page%201-24.pdf bfd46ac49028bcb3afa6567adc4b2ee3 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/2/Full%20text.pdf d687872dc523e489990dc3b887868c10 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/76733/3/Declaration%20Form.pdf 84e754cbf2e1fb9fe8ad9cadd7b43a61 Universiti Malaysia Perlis (UniMAP) Nanotechnology Biosensors Escherichia coli Institute of Nano Electronic Engineering |
| institution |
Universiti Malaysia Perlis |
| collection |
UniMAP Institutional Repository |
| language |
English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English Malay English English English English English English English |
| advisor |
Uda, Hashim, Prof. Dr. Voon, Chun Hong, Dr. Voon, Chun Hong, Dr. Mohd Irfan, Hatim, Assoc. Prof. Dr. Rozana Aina, Maulat Osman, Dr. Sohibul Anuar, Zainol Murad, Ir. Dr. Mohamad Halim, Abd. Wahid, Dr. Sohibul Anuar, Zainol Murad, Ir. Dr. Asral, Bahari Jambek, Assoc. Prof. Dr. Vithyacharan, Retnasamy, Dr. Mohammad Faridun Naim, Tajuddin, Dr. Muhammad Mokhzaini, Azizan, Dr. Norkharziana, Mohd Nayan, Dr. Mohammad Faridun Naim, Tajuddin, Dr. Idris, Md Noor, Prof. Dr. Rosni, Bakar, Prof. Dr. Nurul Razliana, Abdul Razak Mohd Fitri, Mansor Ahmad Kadri, Junoh, Dr. Mohd Yusoff, Mashor, Prof. Dr. Mohd Shukry, Abdul Majid, Assoc. Prof. Ir. Dr. Hariharan, Muthusamy, Dr. Farrah Aini, Dahalan, Dr. Sam Sung, Ting, Dr. Rozana Aina, Maulat Osman, Dr. Mukhzeer, Mohammad Shahimin, Dr. Mohamad Halim, Abd. Wahid, Dr. Mohd Faizal, Jamlos, Assoc. Prof. Ir. Dr. Asral Bahari, Jambek, Dr. Nor Azura Malini, Ahmad Hambali, Dr. Ahmad Kadri, Junoh, Dr. Nasrul Amri, Mohd Amin, Ir. Dr. Umi Fazara, Md. Ali, Dr. Zulkarnay, Zakaria, Dr. Shahriman, Abu Bakar, Assoc. Prof. Dr. Mohd Sazli, Saad Soh, Ping Jack, Assoc. Prof. Dr. Kenneth, Sundaraj, Assoc. Prof. Ir. Dr. Anuar, Mat Safar, Assoc. Prof. Ir. Dr. Wan Sallha, Yusoff, Dr. Norshahrizan, Nordin, Dr. Muhammad Safizal, Abdullah, Dr. Zulkarnay, Zakaria, Dr. Mohd Suberi, Ab Halim, Dr. Phak Len, Eh Kan, Dr. Muzammil, Jusoh, Assoc. Prof. Dr. Azlin Fazlina, Osman, Dr. Mohd Nazri, Mohd Warip, Ir. Dr. Ong, Soon An, Assoc. Prof. Dr. Kamarudin, Hussin, Brig. Jen. Datuk Prof. Emeritus Dr. Mohd Zukime, Mat Junoh, Dr. Afizah, Ayob, Dr. Fahmi, Muhammad Ridwan, Assoc. Prof. Dr. Tunku Salha, Tunku Ahmad, Dr. Mohd Irfan Hatim, Md Dzahir, Assoc. Prof. Dr. Ruzelita, Ngadiran, Dr. Kamarudin, Hussin, Brig. Jen. Datuk Prof. Emeritus Dr. Kamarudin, Hussin, Brig. Jen. Datuk Prof. Emeritus Dr. Teh, Pei Leng, Dr. Shahriman, Abu Bakar, Assoc. Prof. Dr. |
| topic |
Nanotechnology Biosensors Escherichia coli Silicon carbide Silica Silicon compounds Microwave heating Nanotechnology Nanostructured materials Silicon compounds Silicon polymers Microwave amplifiers Chemical tests and reagents Food -- Biotechnology Nanotechnology Biochemicals engineering Genetically modified foods Enriched foods Energy storage Miniature electronic equipment Capacitors Dielectric devices Barium metatitanate Energy storage RF to DC converter circuit Wavelength division multiplexing Photonics Fiber optics Electric circuits -- Direct current Electric current converters DNA microarrays Image processing Image processing -- Digital techniques Insulated gate bipolar transistors Bipolar transistors Wire bonding (Electronic packaging) Planar transistors Photovoltaic power systems Hybrid power systems Electric power production Electric circuit analysis Electric circuits Electric power systems Wind turbines Turbines Power resources Wind power Renewable energy sources Electric power systems Photovoltaic power systems Electric circuit analysis Nanotechnology Entrepreneurship Personality Human capital Microfinance Microfinance -- Bangladesh Solder and soldering Atmospheric temperature Employees -- Recruiting Civil service Personnel management Civil service -- Personnel management Demography -- Indonesia Economic development -- Mathematical models Indonesia -- Population Indonesia -- Statistics Microcomputers Raspberry Pi (Computer) Cardiovascular system -- Diseases Heart -- Abnormalities Wireless communication systems Fibrous composites Composite materials Fibers Hybridization Kenaf Multisensor data fusion Electromyography Signal processing Hamstring muscle Muscle fatigue Granulite Aerobic granules Latex wastewater sludge Jackfruit Jackfruit seeds flour (JSF) Polymer Polymer blends Solar cells Solar batteries Photovoltaic effect Gallium Arsenide (GaAs) Silicon (Si) Photolithography Photoresists Integrated Optical Mach-Zehnder Interferometer (IO-MZI) Telecommunication systems Optical amplifiers Photonics Photonics logic gates Logic gates Ultra-wideband antennas Ultra-wideband communication systems Antennas Ultra wideband (UWB) UWB antennas Wireless sensor networks Digital video Motion detectors Image processing -- Digital techniques Optical fiber communication Brillouin scattering Bragg gratings Transportation noise Noise control Noise Vehicle design Vibration Heat exchangers Tubes Shell-and-tube heat exchangers (STHEX) Heat Nanotechnology Cerbera Sea mango (Cerbera odollam) Carbon dioxide (CO2) Adsorption Spectrum analysis Magnetic induction Nanotechnology Magnetic Induction spectroscopy (MIS) Internal combustion engines Combustion Exhaust systems Flexible structures Balance beam Structural engineering Beam Antennas (Electronics) Satellites Artificial satellites in telecommunication S-band CubeSat Electromyography Triceps brachii (TB) Muscle TB muscle Free space optical interconnects Optical wave guides Free space optics (FSO) Data transfer Accounting -- Jordan Auditing Audit quality Tax evasions Business enterprises -- Nigeria Nanotechnology Small business Business development companies Labor turnover Work-life balance Corporate culture Emotional intelligence Magnetic induction Hydrogen-ion concentration Magnetic induction spectroscopy pH measurement Business logistic Supply Chain Management (SCM) Supply chain Business -- Bangladesh Retail chain stores -- Bangladesh Orthogonal frequency division multiplexing Field programmable gate arrays Radon transforms Wireless communication systems Data transmission Radio direction finders Software radio Wireless communication systems Adaptive signal processing Antenna arrays Angle of Arrival (AOA) Polymeric composites Polyanilines Thermoplastics Composite materials Electric conductivity Filler Polyaniline (PAni) Wireless sensor networks Natural computation Wireless technology Computer communication systems MAC protocol Biomass Biomass energy Microbial fuel cell (MFC) Microbial biotechnology Fuel cells Fly Ash Polymerization Polymer-impregnated concrete Lightweight concrete Bricks Geopolymer Customer loyalty Consumer satisfaction Hotel management Hotels -- Malaysia Hotels -- Jordan Pavements Nanotechnology Nanotechnology Nanotechnology Greenhouse gases Highway engineering Water quality management Water -- Purification Water resources Food prices Power resources -- Costs Petroleum as fuel Foreign exchange rates Oil price Polymeric membranes Butanol Pervaporation Polymeric poly (vinylidene fluoride) (PVDF) Membrane Polymer membrane Digital images -- Watermarking Field programmable gate arrays Watermark Digital watermark Kaolin Ceramics Inorganic polymers Clay minerals Lightweight materials Geopolymer Coatings Kaolin Inorganic polymers Lumber Geopolymer Microcrystalline polymers Cellulose Biopolymers Nypa palm Biodegradation Cellulose -- Biodegradation Satellites RazakSAT-2 Satellite mission Floating Reference Frame |
| spellingShingle |
Nanotechnology Biosensors Escherichia coli Silicon carbide Silica Silicon compounds Microwave heating Nanotechnology Nanostructured materials Silicon compounds Silicon polymers Microwave amplifiers Chemical tests and reagents Food -- Biotechnology Nanotechnology Biochemicals engineering Genetically modified foods Enriched foods Energy storage Miniature electronic equipment Capacitors Dielectric devices Barium metatitanate Energy storage RF to DC converter circuit Wavelength division multiplexing Photonics Fiber optics Electric circuits -- Direct current Electric current converters DNA microarrays Image processing Image processing -- Digital techniques Insulated gate bipolar transistors Bipolar transistors Wire bonding (Electronic packaging) Planar transistors Photovoltaic power systems Hybrid power systems Electric power production Electric circuit analysis Electric circuits Electric power systems Wind turbines Turbines Power resources Wind power Renewable energy sources Electric power systems Photovoltaic power systems Electric circuit analysis Nanotechnology Entrepreneurship Personality Human capital Microfinance Microfinance -- Bangladesh Solder and soldering Atmospheric temperature Employees -- Recruiting Civil service Personnel management Civil service -- Personnel management Demography -- Indonesia Economic development -- Mathematical models Indonesia -- Population Indonesia -- Statistics Microcomputers Raspberry Pi (Computer) Cardiovascular system -- Diseases Heart -- Abnormalities Wireless communication systems Fibrous composites Composite materials Fibers Hybridization Kenaf Multisensor data fusion Electromyography Signal processing Hamstring muscle Muscle fatigue Granulite Aerobic granules Latex wastewater sludge Jackfruit Jackfruit seeds flour (JSF) Polymer Polymer blends Solar cells Solar batteries Photovoltaic effect Gallium Arsenide (GaAs) Silicon (Si) Photolithography Photoresists Integrated Optical Mach-Zehnder Interferometer (IO-MZI) Telecommunication systems Optical amplifiers Photonics Photonics logic gates Logic gates Ultra-wideband antennas Ultra-wideband communication systems Antennas Ultra wideband (UWB) UWB antennas Wireless sensor networks Digital video Motion detectors Image processing -- Digital techniques Optical fiber communication Brillouin scattering Bragg gratings Transportation noise Noise control Noise Vehicle design Vibration Heat exchangers Tubes Shell-and-tube heat exchangers (STHEX) Heat Nanotechnology Cerbera Sea mango (Cerbera odollam) Carbon dioxide (CO2) Adsorption Spectrum analysis Magnetic induction Nanotechnology Magnetic Induction spectroscopy (MIS) Internal combustion engines Combustion Exhaust systems Flexible structures Balance beam Structural engineering Beam Antennas (Electronics) Satellites Artificial satellites in telecommunication S-band CubeSat Electromyography Triceps brachii (TB) Muscle TB muscle Free space optical interconnects Optical wave guides Free space optics (FSO) Data transfer Accounting -- Jordan Auditing Audit quality Tax evasions Business enterprises -- Nigeria Nanotechnology Small business Business development companies Labor turnover Work-life balance Corporate culture Emotional intelligence Magnetic induction Hydrogen-ion concentration Magnetic induction spectroscopy pH measurement Business logistic Supply Chain Management (SCM) Supply chain Business -- Bangladesh Retail chain stores -- Bangladesh Orthogonal frequency division multiplexing Field programmable gate arrays Radon transforms Wireless communication systems Data transmission Radio direction finders Software radio Wireless communication systems Adaptive signal processing Antenna arrays Angle of Arrival (AOA) Polymeric composites Polyanilines Thermoplastics Composite materials Electric conductivity Filler Polyaniline (PAni) Wireless sensor networks Natural computation Wireless technology Computer communication systems MAC protocol Biomass Biomass energy Microbial fuel cell (MFC) Microbial biotechnology Fuel cells Fly Ash Polymerization Polymer-impregnated concrete Lightweight concrete Bricks Geopolymer Customer loyalty Consumer satisfaction Hotel management Hotels -- Malaysia Hotels -- Jordan Pavements Nanotechnology Nanotechnology Nanotechnology Greenhouse gases Highway engineering Water quality management Water -- Purification Water resources Food prices Power resources -- Costs Petroleum as fuel Foreign exchange rates Oil price Polymeric membranes Butanol Pervaporation Polymeric poly (vinylidene fluoride) (PVDF) Membrane Polymer membrane Digital images -- Watermarking Field programmable gate arrays Watermark Digital watermark Kaolin Ceramics Inorganic polymers Clay minerals Lightweight materials Geopolymer Coatings Kaolin Inorganic polymers Lumber Geopolymer Microcrystalline polymers Cellulose Biopolymers Nypa palm Biodegradation Cellulose -- Biodegradation Satellites RazakSAT-2 Satellite mission Floating Reference Frame Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| description |
Breakthrough in nanotechnology provides a great extent in biosensor development and
application. Previous studies showed that nanogap sensor device provides excellent
electrical behavior in sensing biomolecules samples. Nanogap sensor is a device having
a pair of electrodes facing each other, which a molecule trapped in between its will be
identified by observing the electrical characterization. Conventional development
process requires prolonged and tedious compulsory additional method. Thus this
research project focus on developing various size of uniform nanogap structure in
nanometre scales which are capable of sensing Escherichia coli O157:H7 (E. coli
O157:H7) at a low concentration level. The development of the device was divided into
nanogap structure and gold pad structure process using electron beam lithography
(EBL) method and conventional photolithography method respectively. Silicon on
insulator (SOI) substrate was used to fabricate the nanogap structure and gold was used
as a gold pad for a probing purpose. The developed nanogap devices was physically
characterized by Field Emission Scanning Electron Microscopy and Scanning Electron
Microscope. Meanwhile, the performance of the devices was tested by evaluating the
capacitance and impedance reading by sweeping a frequency from 1M Hz to 0.1 Hz at
room temperature with 1.0 mV input using Dielectric Analyzer. The devices were tested
with de-ionized water and different pH level to optimize the sensor sensitivity that
related to the nanogap size. Prior to the detection of E. coli deoxyribonucleic acid
(DNA), the device was surface modified with NH2-Amine functionalized silane group
using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde for DNA to be
covalently bonded with the APTES modified surface. The principle of the E. coli
detection is based on charge density changes of the DNA probe immobilization and
DNA target hybridization on the modified surface. The morphological testing results
shows that the developed devices were observed with 40, 80 and 100 nm nanogap size.
It was found that, the device with smallest gap size, 40 nm shows the highest sensitivity
and stability compared to the device with bigger gap size, 80 and 100 nm. In this project
40 nm size nanogap device was successfully developed as biosensor for E. coli O157:
H7 detection with capability to distinguish the impedance value between
complementary, non-complementary and single mismatch DNA sequences. In addition,
the device was able to detect E. coli O157: H7 DNA target at concentration limit from
10 nM to 1 pM with linear regression equation is ( ) = 3 × 10−7 + 5 × 10−9
and the correlation coefficient is 0.98. |
| format |
Thesis |
| title |
Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| title_short |
Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| title_full |
Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| title_fullStr |
Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| title_full_unstemmed |
Development of silicon on insulator based nanogap sensor for Escherichia Coli O157:H7 detection |
| title_sort |
development of silicon on insulator based nanogap sensor for escherichia coli o157:h7 detection |
| granting_institution |
Universiti Malaysia Perlis (UniMAP) |
| granting_department |
Institute of Nano Electronic Engineering |
| url |
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