Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold

The mechanical, thermal, morphological properties and in vitro degradation study of a 3D porous Pennisetum purpureum (PP)/polylactic acid (PLA) based scaffold were investigated. In this study, a novel scaffold containing P. purpureum and PLA was produced using of the solvent casting and particulate...

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Format:	Thesis
Language:	English
Subjects:	Tissue scaffolds Tissue engineering Pennisetum purpureum Articular cartilage Biomedical engineering
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/4/Revati.pdf
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spelling	my-unimap-779952023-03-06T03:49:20Z Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold Mohd Shukry, Abdul Majid, Ir. Dr. The mechanical, thermal, morphological properties and in vitro degradation study of a 3D porous Pennisetum purpureum (PP)/polylactic acid (PLA) based scaffold were investigated. In this study, a novel scaffold containing P. purpureum and PLA was produced using of the solvent casting and particulate leaching method. PLA composite with various P. purpureum contents (10 wt%, 20 wt%, and 30 wt%) were prepared and subsequently characterised. The morphologies, structures and thermal behaviours of the prepared composite scaffolds were characterised using field-emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The morphology was studied using FESEM; the scaffold possessed 70-200 μm-sized pores and had a greater porosity factor (99%) with a high level of interconnectivity. The mechanical properties and in vitro degradation of the developed porous scaffolds were further characterized. Compression tests were conducted to evaluate the compressive strength and modulus of the scaffolds, according to ASTM F451-95. The compression strength of the scaffolds was found to increase from 1.94 to 9.32 MPa, while the compressive modulus increased from 1.73 to 5.25 MPa as the fillers’ content increased from 0 wt% to 30 wt%. In this study, the synthesized composite scaffolds were immersed in a PBS solution at 37 °C for 40 days. Interestingly, the degradation rate was reduced for the PLA/PP20 scaffold, though insignificantly, this could be attributed to the improved mechanical properties and stronger fibre-matrix interface. Microstructure changes after degradation were observed using FESEM. The FESEM results indicated that a strong fibrematrix interface was formed in the PLA/PP20 scaffold, which reflected the addition of P. purpureum into PLA decreasing the degradation rate compared to in pure PLA scaffolds. From the results, it can be concluded that the properties of the highly porous P. purpureum/PLA scaffold developed in this study can be controlled and optimized. This can be used to facilitate the construction of implantable tissue-engineered cartilage. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77995 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/1/Page%201-24.pdf 4ab8813976c0fc9088b08d5959b615bd http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/2/Full%20text.pdf 00b09484bf1fca71513c713d6b303043 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/4/Revati.pdf 7975f738b97240b2a07ded14da6e175e Universiti Malaysia Perlis (UniMAP) Tissue scaffolds Tissue engineering Pennisetum purpureum Articular cartilage Biomedical engineering School of Mechatronic Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Mohd Shukry, Abdul Majid, Ir. Dr.
topic	Tissue scaffolds Tissue engineering Pennisetum purpureum Articular cartilage Biomedical engineering
spellingShingle	Tissue scaffolds Tissue engineering Pennisetum purpureum Articular cartilage Biomedical engineering Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
description	The mechanical, thermal, morphological properties and in vitro degradation study of a 3D porous Pennisetum purpureum (PP)/polylactic acid (PLA) based scaffold were investigated. In this study, a novel scaffold containing P. purpureum and PLA was produced using of the solvent casting and particulate leaching method. PLA composite with various P. purpureum contents (10 wt%, 20 wt%, and 30 wt%) were prepared and subsequently characterised. The morphologies, structures and thermal behaviours of the prepared composite scaffolds were characterised using field-emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The morphology was studied using FESEM; the scaffold possessed 70-200 μm-sized pores and had a greater porosity factor (99%) with a high level of interconnectivity. The mechanical properties and in vitro degradation of the developed porous scaffolds were further characterized. Compression tests were conducted to evaluate the compressive strength and modulus of the scaffolds, according to ASTM F451-95. The compression strength of the scaffolds was found to increase from 1.94 to 9.32 MPa, while the compressive modulus increased from 1.73 to 5.25 MPa as the fillers’ content increased from 0 wt% to 30 wt%. In this study, the synthesized composite scaffolds were immersed in a PBS solution at 37 °C for 40 days. Interestingly, the degradation rate was reduced for the PLA/PP20 scaffold, though insignificantly, this could be attributed to the improved mechanical properties and stronger fibre-matrix interface. Microstructure changes after degradation were observed using FESEM. The FESEM results indicated that a strong fibrematrix interface was formed in the PLA/PP20 scaffold, which reflected the addition of P. purpureum into PLA decreasing the degradation rate compared to in pure PLA scaffolds. From the results, it can be concluded that the properties of the highly porous P. purpureum/PLA scaffold developed in this study can be controlled and optimized. This can be used to facilitate the construction of implantable tissue-engineered cartilage.
format	Thesis
title	Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
title_short	Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
title_full	Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
title_fullStr	Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
title_full_unstemmed	Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold
title_sort	development and characterization of pennisetum purpureum/pla biocomposite scaffold
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Mechatronic Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77995/4/Revati.pdf
_version_	1776104258358214656

Development and characterization of Pennisetum Purpureum/pla biocomposite scaffold

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