Computational design and development of molecular imprint polymer for selective extraction of andrographolide from andrographis paniculata
This research study highlighted on computational design and molecular imprint polymer development for the extraction of andrographolide from Andrographis paniculata (A. Paniculata). Molecularly Imprinted Polymer (MIP) is one of the “Lock and Key” approach, where MIP is the lock and andrographolid...
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| Format: | Thesis |
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| Language: | English |
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78003/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78003/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78003/4/Hemawathi.pdf |
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| Summary: | This research study highlighted on computational design and molecular imprint polymer
development for the extraction of andrographolide from Andrographis paniculata (A.
Paniculata). Molecularly Imprinted Polymer (MIP) is one of the “Lock and Key”
approach, where MIP is the lock and andrographolide is the key which fits the MIP lock
both physically and chemically. Hyperchem 8.0.10 software was used to simulate and
determine the suitable functional monomer and optimum template-functional monomer
ratio for the best complexity among them. The MIPs were prepared by non-covalent
precipitation polymerization. Andrographolide, methacrylic acid, and ethylene glycol
dimethacrylate were used as template, functional monomer, and cross-linker,
respectively. Non-imprinted polymer (NIP) was developed in the same manner as a
control. The template molecules were removed from MIP polymer particles using
methanol: acetic acid (1:1 v/v) solvent. MIP and NIP were characterized by Fouriertransform
Infrared Spectroscopy, Scanning Electron Microscope and dynamic asorption
study. The efficiency of andrographolide imprinted MIP were evaluated in separation and
sensor applications. For the separation analysis, rebinding assay and adsorption isotherm
studies were conducted. The imprinting factor of MIP is 2.26 with cavities distribution
of 55.45 ml.g-1. The binding assay was analyzed by three types of non-linear adsorption
isotherm. The developed MIP follows Langmuir-Freundlich isotherm with maximum
binding capacity of 149.59 μg.g-1 where the experimental binding capacity was calculated
as 167.86 μg.g-1. MIP-SPME was used to extract andrographolide from A. paniculata
with 92.3 % of recovery. The LOD and LOQ of MIP-SPME is 0.14 and 0.466 μg.ml-1,
respectively. MIP was applied in sensor development using Quartz Crystal Microbalance
(QCM) 200 where MIP was electrodeposited using cyclic voltammetry. QCM 200 device
gives the frequency changes corresponding to the selective adsorption of andrographolide
from A. panciulata. The maximum binding sites on the MIP-QCM sensor by applying
linear Langmuir isotherm is 18.02 ng.cm-2. In addition, MIP-QCM sensor could be used
in real sample analysis. It was found that 45.53 % of andrographolide detected in 0.10
μg.ml-1 of plant extract with LOD and LOQ of 1.206 ng.cm-2 and 4.020 ng.cm-2
respectively. This is the first research using MIP based QCM sensor for the quantification
of andrographolide from A. paniculata. This project demonstrated that the
andrographolide imprinted polymer can be applied both in analytical separation and
sensor development for the detection and quantification of andrographolide from A.
paniculata plant extract. Therefore, a novel molecular imprint polymer was synthesized
by precipitation polymerization which could be applied in separation and sensor studies |
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