Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
Medicinal plants are used traditionally in treatment of various kinds of diseases since time immemorial. The present study was carried out to evaluate the antimicrobial and antioxidant activities of roots and leaves of Elephantopus scaber by using different solvent (ethyl alcohol ,acetone and wat...
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| Format: | Thesis |
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| Language: | English |
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| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78034/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78034/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78034/3/Moh%20Riyadh.pdf |
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| Summary: | Medicinal plants are used traditionally in treatment of various kinds of diseases since time
immemorial. The present study was carried out to evaluate the antimicrobial and
antioxidant activities of roots and leaves of Elephantopus scaber by using different
solvent (ethyl alcohol ,acetone and water). The ethyl alcohol, acetone and water extracts of
the roots and leaves were subjected to phytochemical analyses, which revealed the
presence of alkaloids, phenols, flavonoids, saponins, steroids, tannins, coumarins,
quinones and glycosides in most of the selected of roots and leaves useing different
solvents. Ethyl alcohol, acetone and water extracts of roots and leaves were investigated
for in.vitro.antimicrobial activity against Escherichia coli, Salmonella typhis, Klebsiella
pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and Candida Albican by
well diffusion method using different concentrations (20, 40, 60, 80, 100)mg/ml. Bioassay
data obtained from the active extracts and oils were subjected to statistical analysis of
variance (ANOVA). Treatment means showing significant difference (p ≤ 0.05) were
separated using Student-Newman-Keuls test (SNK). Ethyl alcohol and acetone extract of
roots showed promising antimicrobial activity against all test organisms. Ethyl alcohol
extract of root gave a significant effect activity against all test organisms compared with
the other sample. Ethyl alcohol extract of leaves was also higher compared with the
acetone extract of roots for a concentrations of 100 and 20mg/ml. The water extract of
leaves has a weak activity against all test organisms and did not inhibit the growth of
Klebsiella pneumonia for a concentrations of 100mg/ml but at a concentrations of
20mg/ml did not inhibit the growth of all test organisms. The Minimum inhibitory
concentration (MIC) of the roots and leaves extracts by using different solvent were also
determined against different test organisms. The MIC value of roots and leaves extract
ranged from 100 mg/ml to 500 mg/ml. The ethyl alcohol, acetone and water extract of
roots and leaves were selected for possible antimicrobial activity. The antimicrobial
activity was studied against six microorganisms namely Escherichia coli, Salmonella
typhis, Klebsiella pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and
Candida Albican. It was found that ethyl alcohol extract of roots showed height influence
against Escherichia coli, Salmonella typhis, Streptococcus pyogenes, Staphylococcus
aureus, and Candida Albican but less effective against Klebsiella pneumonia. The water
extract showed a weak effect against all tests organisms and did not has any effect against
Klebsiella pneumonia. The roots and leaves extract from Elephantopus scaber using
different solvents to extract were evaluated for antioxidant activity by using DPPH method
using a different concentrations (1, 10, 20, 30, 40, 60, 80, 100) μg/ml. The results from the
analysis were compared with ascorbic acid as standard. |
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