Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection

Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global choc...

Full description

Saved in:

Bibliographic Details
Main Author:	Joel Michael, Ponniah
Format:	Thesis
Language:	English English
Published:	2015
Subjects:	Q Science (General) SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/10926/1/Development%20of%20Recombinant%20Plant%20Vaccine%20for%20Cocoa%20Swollen%20Shoot%20Virus%20%28CSSV%29%20Infection%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/10926/8/Joel%20Michael%20Pnnniah%20ft.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.10926
record_format	uketd_dc
spelling	my-unimas-ir.109262023-11-21T06:22:01Z Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection 2015 Joel Michael, Ponniah Q Science (General) SB Plant culture Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global chocolate industry. A prospective vaccine against CSSD was developed in this study using recombinant gene technology. Agou1 cacao swollen shoot virus genome was isolated from total plant DNA using an established PCRbased isolation methodology, and the resultant full-length linear virus DNA amplified in OneShot® Top10 chemically competent Escherichia coli cells using pCR-XL TOPO® cloning plasmids. The virus DNA was later retrieved, digested using EcoRI restriction enzyme, and the targeted ORF3 gene fragment isolated through gel electrophoresis and incorporated into pBAD-TOPO® expression plasmids. This were then transformed into domesticated Paenibacillus polymyxa cells through electroporation, which acted as vaccine carrier. The resultant Biotic Response Elicitor Vaccine (BREV) produced a fragment of the virus particle, which was hypothesized to induce augmented systemic expression of the natural plant defense mechanism. 2015 Thesis http://ir.unimas.my/id/eprint/10926/ http://ir.unimas.my/id/eprint/10926/1/Development%20of%20Recombinant%20Plant%20Vaccine%20for%20Cocoa%20Swollen%20Shoot%20Virus%20%28CSSV%29%20Infection%20%2824%20pages%29.pdf text en public http://ir.unimas.my/id/eprint/10926/8/Joel%20Michael%20Pnnniah%20ft.pdf text en validuser masters Universiti Malaysia Sarawak, (UNIMAS) Faculty of Resources Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English
topic	Q Science (General) SB Plant culture
spellingShingle	Q Science (General) SB Plant culture Joel Michael, Ponniah Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
description	Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global chocolate industry. A prospective vaccine against CSSD was developed in this study using recombinant gene technology. Agou1 cacao swollen shoot virus genome was isolated from total plant DNA using an established PCRbased isolation methodology, and the resultant full-length linear virus DNA amplified in OneShot® Top10 chemically competent Escherichia coli cells using pCR-XL TOPO® cloning plasmids. The virus DNA was later retrieved, digested using EcoRI restriction enzyme, and the targeted ORF3 gene fragment isolated through gel electrophoresis and incorporated into pBAD-TOPO® expression plasmids. This were then transformed into domesticated Paenibacillus polymyxa cells through electroporation, which acted as vaccine carrier. The resultant Biotic Response Elicitor Vaccine (BREV) produced a fragment of the virus particle, which was hypothesized to induce augmented systemic expression of the natural plant defense mechanism.
format	Thesis
qualification_level	Master's degree
author	Joel Michael, Ponniah
author_facet	Joel Michael, Ponniah
author_sort	Joel Michael, Ponniah
title	Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
title_short	Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
title_full	Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
title_fullStr	Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
title_full_unstemmed	Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
title_sort	development of recombinant plant vaccine for cacao swollen shoot virus (cssv) infection
granting_institution	Universiti Malaysia Sarawak, (UNIMAS)
granting_department	Faculty of Resources Science and Technology
publishDate	2015
url	http://ir.unimas.my/id/eprint/10926/1/Development%20of%20Recombinant%20Plant%20Vaccine%20for%20Cocoa%20Swollen%20Shoot%20Virus%20%28CSSV%29%20Infection%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/10926/8/Joel%20Michael%20Pnnniah%20ft.pdf
_version_	1783728088235900928

Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection

Similar Items