Development of single chain variable fragment antibodies against dengue virus
The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned in...
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| Format: | Thesis |
| Language: | English |
| Published: |
2006
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| Online Access: | http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf |
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| Summary: | The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens. |
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