Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak

The presence of Vibrio parahaemolyticus in marketed seafood and surface water samples in Kuching-Samarahan District, Sarawak were examined over 6 months period (December 2003 to May 2004). During the sampling period, a total of 81 marketed seafood samples and 114 surface water samples were collected...

Full description

Saved in:

Bibliographic Details
Main Author:	Ng, Lee Tze
Format:	Thesis
Language:	English
Published:	2007
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/id/eprint/14238/1/Ng%20Lee%20Tze%20ft.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.14238
record_format	uketd_dc
spelling	my-unimas-ir.142382023-06-14T01:21:08Z Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak 2007 Ng, Lee Tze Q Science (General) The presence of Vibrio parahaemolyticus in marketed seafood and surface water samples in Kuching-Samarahan District, Sarawak were examined over 6 months period (December 2003 to May 2004). During the sampling period, a total of 81 marketed seafood samples and 114 surface water samples were collected. Based on morphological, biochemical and genotype identification, 50% of the marketed seafood samples and 62% of the surface water samples were positive for V. parahaemolyticus, yielding 69 and 87 isolates respectively. High occurrence of V. parahaemolyticus was observed in both the marketed seafood and surface water samples. Out of 69 confirmed V. parahaemolyticus isolates, 34 isolates from marketed seafood and another 49 isolates from surface water samples (out of 87 isolates) were selected for further analysis) Their antibiotic resistance, presence of plasmid(s), DNA fingerprinting using RAPD-PCR (Randomly Amplified Polymorphic DNA), and ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus sequence or ERIC), and the presence of virulence genes <thermostabile direct hemolysin gene, tdh, and TDH-related hemolysin gene, trh) were investigated. Based on the ERIC primer, DNA sequencing was performed on 12 of the selected isolates. All the V. parahaemolyticus isolates showed different resistancy towards twelve antimicrobial agents tested. The Multiple Antibiotic Resistance (MAR) index calculated ranged from 0.50 to 0.92. The isolates were differentiated into 20 patterns through plasmid profiling, with plasmid sizes ranging from 3.1 to 63.0 kb. Results revealed that there is no clear correlation between the plasmid profiles and the antibiotic resistance patterns. RAPD and ERIC-PCR analysis successfully subtyped all the 83 isolates. RAPD and ERIC clustering analysis managed to differentiate the isolates into different clusters in which the isolates were distributed based on the month of sampling. Based on the Simpson's index of diversity, results from both RAPD and ERIC methods were almost similar, 99.05% and 99.00%, respectively. Virulence genes detection via specific PCR showed that only 10.8% of the isolates were positive for trh gene, whereas no isolate was positive for the tdh gene. The presence of trh gene in this organism indicates potential risk towards public health in event of an outbreak. Universiti Malaysia Sarawak (UNIMAS) 2007 Thesis http://ir.unimas.my/id/eprint/14238/ http://ir.unimas.my/id/eprint/14238/1/Ng%20Lee%20Tze%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ng, Lee Tze Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
description	The presence of Vibrio parahaemolyticus in marketed seafood and surface water samples in Kuching-Samarahan District, Sarawak were examined over 6 months period (December 2003 to May 2004). During the sampling period, a total of 81 marketed seafood samples and 114 surface water samples were collected. Based on morphological, biochemical and genotype identification, 50% of the marketed seafood samples and 62% of the surface water samples were positive for V. parahaemolyticus, yielding 69 and 87 isolates respectively. High occurrence of V. parahaemolyticus was observed in both the marketed seafood and surface water samples. Out of 69 confirmed V. parahaemolyticus isolates, 34 isolates from marketed seafood and another 49 isolates from surface water samples (out of 87 isolates) were selected for further analysis) Their antibiotic resistance, presence of plasmid(s), DNA fingerprinting using RAPD-PCR (Randomly Amplified Polymorphic DNA), and ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus sequence or ERIC), and the presence of virulence genes <thermostabile direct hemolysin gene, tdh, and TDH-related hemolysin gene, trh) were investigated. Based on the ERIC primer, DNA sequencing was performed on 12 of the selected isolates. All the V. parahaemolyticus isolates showed different resistancy towards twelve antimicrobial agents tested. The Multiple Antibiotic Resistance (MAR) index calculated ranged from 0.50 to 0.92. The isolates were differentiated into 20 patterns through plasmid profiling, with plasmid sizes ranging from 3.1 to 63.0 kb. Results revealed that there is no clear correlation between the plasmid profiles and the antibiotic resistance patterns. RAPD and ERIC-PCR analysis successfully subtyped all the 83 isolates. RAPD and ERIC clustering analysis managed to differentiate the isolates into different clusters in which the isolates were distributed based on the month of sampling. Based on the Simpson's index of diversity, results from both RAPD and ERIC methods were almost similar, 99.05% and 99.00%, respectively. Virulence genes detection via specific PCR showed that only 10.8% of the isolates were positive for trh gene, whereas no isolate was positive for the tdh gene. The presence of trh gene in this organism indicates potential risk towards public health in event of an outbreak.
format	Thesis
qualification_level	Master's degree
author	Ng, Lee Tze
author_facet	Ng, Lee Tze
author_sort	Ng, Lee Tze
title	Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
title_short	Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
title_full	Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
title_fullStr	Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
title_full_unstemmed	Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak
title_sort	genotypic characterization of vibrio parahaemolyticus from seafood and river water in kuching-samarahan district, sarawak
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2007
url	http://ir.unimas.my/id/eprint/14238/1/Ng%20Lee%20Tze%20ft.pdf
_version_	1783728152901582848

Genotypic characterization of vibrio parahaemolyticus from seafood and river water in Kuching-Samarahan District, Sarawak

Similar Items