Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere

Anthracnose caused by Colletotrichum gloeosporioides is a serious disease of black pepper. Yield losses attributed to the infliction of anthracnose disease on young spike and immature berries had been reported to be about 50% in serious case. Different approaches have been used to control this dise...

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Main Author:	Yap, Chin Ann
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/20967/2/Yap%20CA.pdf
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id	my-unimas-ir.20967
record_format	uketd_dc
spelling	my-unimas-ir.209672023-10-02T01:25:38Z Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere 2017 Yap, Chin Ann Q Science (General) QR Microbiology Anthracnose caused by Colletotrichum gloeosporioides is a serious disease of black pepper. Yield losses attributed to the infliction of anthracnose disease on young spike and immature berries had been reported to be about 50% in serious case. Different approaches have been used to control this disease. One of the alternative approaches is through biological control. Colletotrichum gloeosporioides is able to infect and colonize detached pepper leaves and berries and develop infection symptoms 10 days after inoculation. Inoculation on wounded young leaves from Kuching variety with conidial suspension from 15 day olds cultures were the optimal condition for infection in ex vivo assay. The detached leaf inoculation method has proven to be useful for screening bacterial strains as biological control agents of pepper anthracnose. Five indigenous bacterial isolates namely B. subtilis, B. vallismortis, B. amyloliquefaciens and 2 B. atrophoues isolated from soil rhizosphere shown positive results against anthracnose disease caused by C. gloeosporioides. The five (5) Bacillus spp. were effective inhibit the growth of 5 pyhtopathogenic fungi, where control mechanisms involved were through the secretion of protease and cellulase enzyme that are responsible for fungal cell wall hydrolysis. unimas 2017 Thesis http://ir.unimas.my/id/eprint/20967/ http://ir.unimas.my/id/eprint/20967/2/Yap%20CA.pdf text en validuser phd doctoral UNIMAS
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Yap, Chin Ann Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
description	Anthracnose caused by Colletotrichum gloeosporioides is a serious disease of black pepper. Yield losses attributed to the infliction of anthracnose disease on young spike and immature berries had been reported to be about 50% in serious case. Different approaches have been used to control this disease. One of the alternative approaches is through biological control. Colletotrichum gloeosporioides is able to infect and colonize detached pepper leaves and berries and develop infection symptoms 10 days after inoculation. Inoculation on wounded young leaves from Kuching variety with conidial suspension from 15 day olds cultures were the optimal condition for infection in ex vivo assay. The detached leaf inoculation method has proven to be useful for screening bacterial strains as biological control agents of pepper anthracnose. Five indigenous bacterial isolates namely B. subtilis, B. vallismortis, B. amyloliquefaciens and 2 B. atrophoues isolated from soil rhizosphere shown positive results against anthracnose disease caused by C. gloeosporioides. The five (5) Bacillus spp. were effective inhibit the growth of 5 pyhtopathogenic fungi, where control mechanisms involved were through the secretion of protease and cellulase enzyme that are responsible for fungal cell wall hydrolysis.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Yap, Chin Ann
author_facet	Yap, Chin Ann
author_sort	Yap, Chin Ann
title	Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
title_short	Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
title_full	Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
title_fullStr	Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
title_full_unstemmed	Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere
title_sort	biological control of colletotrichum spp. of pepper (piper nigrum l.) using indigenous bacterial isolates from soil rhizosphere
granting_institution	UNIMAS
publishDate	2017
url	http://ir.unimas.my/id/eprint/20967/2/Yap%20CA.pdf
_version_	1783728211184582656

Biological Control of Colletotrichum spp. of Pepper (Piper nigrum L.) using Indigenous Bacterial Isolates from Soil Rhizosphere

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