Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8

Wide spectrum usage of synthetic dyes particularly by the textile and dyestuff industry has caused serious pollution of rivers and lake due to insufficient treatment of dye containing wastewater by current known wastewater treatment methods. Endophytic fungi were therefore isolated from Melastoma ma...

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Main Author:	Ngieng, Ngui Sing
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Q Science (General) QH Natural history QH301 Biology
Online Access:	http://ir.unimas.my/id/eprint/20968/1/Ngieng%20Ngui%20Sing%20ft.pdf
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id	my-unimas-ir.20968
record_format	uketd_dc
spelling	my-unimas-ir.209682023-05-18T08:01:53Z Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8 2017 Ngieng, Ngui Sing Q Science (General) QH Natural history QH301 Biology Wide spectrum usage of synthetic dyes particularly by the textile and dyestuff industry has caused serious pollution of rivers and lake due to insufficient treatment of dye containing wastewater by current known wastewater treatment methods. Endophytic fungi were therefore isolated from Melastoma malabathricum (local name: Senduduk) to test for their ability to decolourise synthetic dyes. Screening on agar plate for synthetic dye decolourisation has enabled the identification of an isolate MS8 that is capable of decolourising all the synthetic dye tested. Synthetic dye decolourisation in liquid medium by MS8 further confirmed the ability of the fungus to decolourise both azo and anthraquinone dyes. Comparing the ITS sequence with NCBI GenBank identified the fungus to be Marasmius cladophyllus. Study on the dye decolourising mechanism of M. cladophyllus in liquid medium using RBBR dye showed the fungus degradative capability in decolourising the dye with no dye adsorption on the fungal mycelium. Further ligninolytic enzyme assay revealed that the presence of RBBR dye induced a 70 folds increase in laccase activity by M. cladophyllus. This laccase enzyme after precipitation by ammonium sulphate can also decolourise 90.2% of RBBR dye, 80.0% of Methyl red, 59.1% of Congo red and 56.1% of Orange G in just 24 hours thus indicating that laccase is the dye decolourising enzyme. unimas 2017 Thesis http://ir.unimas.my/id/eprint/20968/ http://ir.unimas.my/id/eprint/20968/1/Ngieng%20Ngui%20Sing%20ft.pdf text en validuser phd doctoral UNIMAS
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH Natural history QH301 Biology
spellingShingle	Q Science (General) QH Natural history QH301 Biology Ngieng, Ngui Sing Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
description	Wide spectrum usage of synthetic dyes particularly by the textile and dyestuff industry has caused serious pollution of rivers and lake due to insufficient treatment of dye containing wastewater by current known wastewater treatment methods. Endophytic fungi were therefore isolated from Melastoma malabathricum (local name: Senduduk) to test for their ability to decolourise synthetic dyes. Screening on agar plate for synthetic dye decolourisation has enabled the identification of an isolate MS8 that is capable of decolourising all the synthetic dye tested. Synthetic dye decolourisation in liquid medium by MS8 further confirmed the ability of the fungus to decolourise both azo and anthraquinone dyes. Comparing the ITS sequence with NCBI GenBank identified the fungus to be Marasmius cladophyllus. Study on the dye decolourising mechanism of M. cladophyllus in liquid medium using RBBR dye showed the fungus degradative capability in decolourising the dye with no dye adsorption on the fungal mycelium. Further ligninolytic enzyme assay revealed that the presence of RBBR dye induced a 70 folds increase in laccase activity by M. cladophyllus. This laccase enzyme after precipitation by ammonium sulphate can also decolourise 90.2% of RBBR dye, 80.0% of Methyl red, 59.1% of Congo red and 56.1% of Orange G in just 24 hours thus indicating that laccase is the dye decolourising enzyme.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ngieng, Ngui Sing
author_facet	Ngieng, Ngui Sing
author_sort	Ngieng, Ngui Sing
title	Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
title_short	Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
title_full	Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
title_fullStr	Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
title_full_unstemmed	Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8
title_sort	characterisation of synthetic dye decolourising genes and their enzymes produced by an endophytic fungus marasmius cladophyllus umas ms8
granting_institution	UNIMAS
publishDate	2017
url	http://ir.unimas.my/id/eprint/20968/1/Ngieng%20Ngui%20Sing%20ft.pdf
_version_	1783728211611353088

Characterisation of Synthetic Dye Decolourising Genes and their Enzymes Produced by an Endophytic Fungus Marasmius cladophyllus UMAS MS8

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