Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1

LA is extensively being used in the field of phannaceutical, food, chemical, cosmetic and in the production of biodegradable plastic polyLA (PLA). Nitrogen sources such as corn steep liquor (CSL), ammonium sulphate (AS) and urea were tested individually and incorporate with each other with the purpo...

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Main Author:	Nur Fashareena Adila, Abd Rahim
Format:	Thesis
Language:	English English
Published:	2017
Subjects:	QP Physiology
Online Access:	http://ir.unimas.my/id/eprint/30081/1/Nur%20Fashareena%20Adila.pdf http://ir.unimas.my/id/eprint/30081/6/Nur%20Fashareena.pdf
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id	my-unimas-ir.30081
record_format	uketd_dc
spelling	my-unimas-ir.300812024-02-05T01:41:28Z Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1 2017 Nur Fashareena Adila, Abd Rahim QP Physiology LA is extensively being used in the field of phannaceutical, food, chemical, cosmetic and in the production of biodegradable plastic polyLA (PLA). Nitrogen sources such as corn steep liquor (CSL), ammonium sulphate (AS) and urea were tested individually and incorporate with each other with the purpose to stimulate the LA fennentation in comparison with the use of yeast extract (YE). The objective was to reduce the production cost ofLA without affecting the . concentration of LA production by using sago starch as an alternative substrate. The fermentation media consisted of hydrolysed sago starch at an optimum concentration (110 gLI), supplemented with various tested ofnitrogen sources and was inoculated with Lactococcus lactis 10-1. Batch fennentation was carried out by controlling the pH (6.8), temperature (45°C) and agitation (100 x g) in 2 L jar fennenter. Based on the results obtained, 20 gL- 1 of YE supplementation proved to be the most optimum concentration of YE to be supplied into a fermentation media as compared to different concentrations of YEo The LA production was 96.3 gL1 , and the residual glucose was 2.95 gL-I • None of the individually tested nitrogen sources gave LA concentrations comparable to 20 gL-1 YE supplementation. However, the mixture ofnitrogen sources was comparable to the production of LA produced by 20 gL- 1 YEo The concentration of LA production by the mixture of ammonium sulphate: corn steep liquor: yeast extract (AS: CSL: YE) with the ratio of 2:2: 1 was 94.5 gL- 1 with the residual glucose of 4.55 gL-I. The incorporation of AS, CSL and YE generate comparable performance with the medium supplemented only with YE (20 gL- 1 ) and was not statistically different (p>0.05). The results indicated that this mixture ofnitrogen sources could be economically used to minimise the use of YE without affecting the concentration of LA. Universiti Malaysia Sarawak (UNIMAS) 2017 Thesis http://ir.unimas.my/id/eprint/30081/ http://ir.unimas.my/id/eprint/30081/1/Nur%20Fashareena%20Adila.pdf text en public http://ir.unimas.my/id/eprint/30081/6/Nur%20Fashareena.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English
topic	QP Physiology
spellingShingle	QP Physiology Nur Fashareena Adila, Abd Rahim Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
description	LA is extensively being used in the field of phannaceutical, food, chemical, cosmetic and in the production of biodegradable plastic polyLA (PLA). Nitrogen sources such as corn steep liquor (CSL), ammonium sulphate (AS) and urea were tested individually and incorporate with each other with the purpose to stimulate the LA fennentation in comparison with the use of yeast extract (YE). The objective was to reduce the production cost ofLA without affecting the . concentration of LA production by using sago starch as an alternative substrate. The fermentation media consisted of hydrolysed sago starch at an optimum concentration (110 gLI), supplemented with various tested ofnitrogen sources and was inoculated with Lactococcus lactis 10-1. Batch fennentation was carried out by controlling the pH (6.8), temperature (45°C) and agitation (100 x g) in 2 L jar fennenter. Based on the results obtained, 20 gL- 1 of YE supplementation proved to be the most optimum concentration of YE to be supplied into a fermentation media as compared to different concentrations of YEo The LA production was 96.3 gL1 , and the residual glucose was 2.95 gL-I • None of the individually tested nitrogen sources gave LA concentrations comparable to 20 gL-1 YE supplementation. However, the mixture ofnitrogen sources was comparable to the production of LA produced by 20 gL- 1 YEo The concentration of LA production by the mixture of ammonium sulphate: corn steep liquor: yeast extract (AS: CSL: YE) with the ratio of 2:2: 1 was 94.5 gL- 1 with the residual glucose of 4.55 gL-I. The incorporation of AS, CSL and YE generate comparable performance with the medium supplemented only with YE (20 gL- 1 ) and was not statistically different (p>0.05). The results indicated that this mixture ofnitrogen sources could be economically used to minimise the use of YE without affecting the concentration of LA.
format	Thesis
qualification_level	Master's degree
author	Nur Fashareena Adila, Abd Rahim
author_facet	Nur Fashareena Adila, Abd Rahim
author_sort	Nur Fashareena Adila, Abd Rahim
title	Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
title_short	Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
title_full	Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
title_fullStr	Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
title_full_unstemmed	Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1
title_sort	enhanced lactic acid production from hydrolysed sago starch by using different types of nitrogen sources by lactococcus lactis 10-1
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2017
url	http://ir.unimas.my/id/eprint/30081/1/Nur%20Fashareena%20Adila.pdf http://ir.unimas.my/id/eprint/30081/6/Nur%20Fashareena.pdf
_version_	1794023010001223680

Enhanced Lactic Acid Production from Hydrolysed Sago Starch by using Different Types of Nitrogen Sources by Lactococcus lactis 10-1

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