Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media

With the imminent depletion of natural resources, the search for alternatives for diesel has been an ongoing issue in the scientific world. Biodiesel feedstock, such as microbial oil, can be generated from fungi, yeast and algae. However, suitable environment must be provided to ensure optimal growt...

Full description

Saved in:

Bibliographic Details
Main Author:	Afizul Safwan, Azahari
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/31385/1/Afizul%20Safwan%20Bin%20Azahari%20ft.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.31385
record_format	uketd_dc
spelling	my-unimas-ir.313852023-03-06T02:44:53Z Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media 2020-08-26 Afizul Safwan, Azahari QR Microbiology With the imminent depletion of natural resources, the search for alternatives for diesel has been an ongoing issue in the scientific world. Biodiesel feedstock, such as microbial oil, can be generated from fungi, yeast and algae. However, suitable environment must be provided to ensure optimal growth for efficient production of microbial oil. In this study, two different growth media, yeast malt broth (YMB) and Wild medium were tested for the growth of Lipomyces starkeyi ATCC 12659 and its mutant strains. The mutant strains tested were L. starkeyi MV-01 and L. starkeyi MV-05. L. starkeyi ATCC 12659 produced 8.62 g/L biomass in YMB and 8.51 g/L in Wild media (1.3% difference). A significantly higher yield was recorded for L. starkeyi MV-01 when grown in YMB (9.16 g/L), compared to Wild media (6.44 g/L). As for L. starkeyi MV-05, a lower biomass yield was recorded when grown in YMB (9.62 g/L) than when grown in Wild medium (11.23 g/L). L. starkeyi ATCC 12659 was found to accumulate lipid up to 27.78% (w/w) of its biomass at 8.07 g/L. L. starkeyi MV-01 accumulated lipid at 22.46% (w/w) of its biomass at 7.82 g/L and L. starkeyi MV-05 showed a peak lipid accumulation of 25.89% (w/w) at 8.60 g/L. When grown in sago effluent, L. starkeyi ATCC 12659 efficiently hydrolyzed starch to lipid at 35.04% (w/w) (9.47 g/L) of its dry biomass. L. starkeyi MV-01 was able to produce lipid at 33.62% (w/w) (8.03 g/L) of its dry biomass while L. starkeyi MV-05 converted starch to lipid at 34.11% (w/w) at 9.80 g/L dry biomass. Further studies that focuses on optimizing the cultivation conditions such as the types of carbon sources, carbon to nitrogen ratio, aeration inside the fermentation broth, pH and temperature can be conducted as these attributes greatly affect the microbial lipid yield. These include can greatly. In conclusion, parental strain L. starkeyi showed the highest amount of lipid accumulated when compared with its mutant strains for both sago starch and sago effluent substrate. 2020-08 Thesis http://ir.unimas.my/id/eprint/31385/ http://ir.unimas.my/id/eprint/31385/1/Afizul%20Safwan%20Bin%20Azahari%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Afizul Safwan, Azahari Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
description	With the imminent depletion of natural resources, the search for alternatives for diesel has been an ongoing issue in the scientific world. Biodiesel feedstock, such as microbial oil, can be generated from fungi, yeast and algae. However, suitable environment must be provided to ensure optimal growth for efficient production of microbial oil. In this study, two different growth media, yeast malt broth (YMB) and Wild medium were tested for the growth of Lipomyces starkeyi ATCC 12659 and its mutant strains. The mutant strains tested were L. starkeyi MV-01 and L. starkeyi MV-05. L. starkeyi ATCC 12659 produced 8.62 g/L biomass in YMB and 8.51 g/L in Wild media (1.3% difference). A significantly higher yield was recorded for L. starkeyi MV-01 when grown in YMB (9.16 g/L), compared to Wild media (6.44 g/L). As for L. starkeyi MV-05, a lower biomass yield was recorded when grown in YMB (9.62 g/L) than when grown in Wild medium (11.23 g/L). L. starkeyi ATCC 12659 was found to accumulate lipid up to 27.78% (w/w) of its biomass at 8.07 g/L. L. starkeyi MV-01 accumulated lipid at 22.46% (w/w) of its biomass at 7.82 g/L and L. starkeyi MV-05 showed a peak lipid accumulation of 25.89% (w/w) at 8.60 g/L. When grown in sago effluent, L. starkeyi ATCC 12659 efficiently hydrolyzed starch to lipid at 35.04% (w/w) (9.47 g/L) of its dry biomass. L. starkeyi MV-01 was able to produce lipid at 33.62% (w/w) (8.03 g/L) of its dry biomass while L. starkeyi MV-05 converted starch to lipid at 34.11% (w/w) at 9.80 g/L dry biomass. Further studies that focuses on optimizing the cultivation conditions such as the types of carbon sources, carbon to nitrogen ratio, aeration inside the fermentation broth, pH and temperature can be conducted as these attributes greatly affect the microbial lipid yield. These include can greatly. In conclusion, parental strain L. starkeyi showed the highest amount of lipid accumulated when compared with its mutant strains for both sago starch and sago effluent substrate.
format	Thesis
qualification_level	Master's degree
author	Afizul Safwan, Azahari
author_facet	Afizul Safwan, Azahari
author_sort	Afizul Safwan, Azahari
title	Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
title_short	Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
title_full	Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
title_fullStr	Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
title_full_unstemmed	Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media
title_sort	lipid accumulation in lipomyces starkeyi mutants cultured in sago media
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2020
url	http://ir.unimas.my/id/eprint/31385/1/Afizul%20Safwan%20Bin%20Azahari%20ft.pdf
_version_	1783728399206842368

Lipid accumulation in Lipomyces starkeyi mutants cultured in sago media

Similar Items