Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser

ABSTRACT Neolamarckia cadamba (a.k.a Kelampayan) is one of the fast-growing plantation tree species for reforestation programme and planted forest development in Sarawak. It is one of the best sources of raw material for the plywood industry, while its leaves and bark have medical applications. Hen...

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Main Author:	Maslini, Japar Ali
Format:	Thesis
Language:	English
Published:	2020
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/31419/2/Tissue%20Culture%20of%20Neolamarckia%20cadamba%20%28Roxb.%29%20Bosser.pdf
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spelling	my-unimas-ir.314192023-03-20T08:07:18Z Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser 2020-07-23 Maslini, Japar Ali SB Plant culture ABSTRACT Neolamarckia cadamba (a.k.a Kelampayan) is one of the fast-growing plantation tree species for reforestation programme and planted forest development in Sarawak. It is one of the best sources of raw material for the plywood industry, while its leaves and bark have medical applications. Hence, this study aimed to establish protocols for in vitro seed germination, seedling growth, callus induction, direct and indirect shoot regeneration of N. cadamba. Seed propagation of N. cadamba is limited due to the lack of viable seed production, thus leading to low germination rate, and the vegetative propagation of N. cadamba by traditional means is not successful. The results showed the best media for seed germination, and seedling growth of N. cadamba was full-strength Gamborgs (B5) medium. Seedling growth and shoot multiplication of N. cadamba were given a positive response to BAP or NAA at 1.0 mg/L, respectively. The combination of plant growth regulators (PGRs) with 10% (v/v) of coconut water (CW) gave the highest percentage of seedling growth under the culture condition 16-h light/ 8-h darkness at room temperature. Also, the combination of PGRs with CW gave a reasonable response to the callus induction, direct and indirect shoot regeneration of N. cadamba. In vitro seedling of N. cadamba grew well under 16-h light/ 8-h dark at room temperature. The good callogenic response was found in a culture that exposed to the 24-h dark treatment (88.9%). However, the 24-h light treatment resulted in a poor callogenic response (22.2%). The good callogenic response was found in B5 medium supplemented with a low concentration of auxins and cytokinins which was 1.0 mg/L of BAP (86.7%) and NAA (80.0%) within eight weeks of inoculation. Indirect shoot regeneration from calli derived from the young leaf explants was successfully initiated by inoculating callus onto B5 medium fortified with combinations of 2.0 mg/L BAP and 0.5 mg/L NAA. BAP 2.0 mg/L added with 10% coconut water also can help to promote cell regeneration, division and differentiation of meristem cell. N. cadamba shoots were actively regenerated when the nodal segments were inoculated on medium added with 1.0 mg/L BAP and 10% CW. About 97.8% of nodal segments were able to regenerate shoots, and an average of 19 shoots per explant was produced after 16 weeks of culture. The present study has successfully established the tissue culture protocol for propagating N. cadamba. This newly established protocol could pave the way to overcome the shortage of planting materials for planted forest development in Sarawak, which in turn assures the local timber industries to meet the global demand for wood-related products. Keywords: Neolamarckia cadamba, complex additives, in vitro seed germination, seedling growth, callus induction, indirect and direct shoot regeneration, plantlet regeneration. 2020-07 Thesis http://ir.unimas.my/id/eprint/31419/ http://ir.unimas.my/id/eprint/31419/2/Tissue%20Culture%20of%20Neolamarckia%20cadamba%20%28Roxb.%29%20Bosser.pdf text en validuser phd doctoral Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology my brain 15
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Maslini, Japar Ali Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
description	ABSTRACT Neolamarckia cadamba (a.k.a Kelampayan) is one of the fast-growing plantation tree species for reforestation programme and planted forest development in Sarawak. It is one of the best sources of raw material for the plywood industry, while its leaves and bark have medical applications. Hence, this study aimed to establish protocols for in vitro seed germination, seedling growth, callus induction, direct and indirect shoot regeneration of N. cadamba. Seed propagation of N. cadamba is limited due to the lack of viable seed production, thus leading to low germination rate, and the vegetative propagation of N. cadamba by traditional means is not successful. The results showed the best media for seed germination, and seedling growth of N. cadamba was full-strength Gamborgs (B5) medium. Seedling growth and shoot multiplication of N. cadamba were given a positive response to BAP or NAA at 1.0 mg/L, respectively. The combination of plant growth regulators (PGRs) with 10% (v/v) of coconut water (CW) gave the highest percentage of seedling growth under the culture condition 16-h light/ 8-h darkness at room temperature. Also, the combination of PGRs with CW gave a reasonable response to the callus induction, direct and indirect shoot regeneration of N. cadamba. In vitro seedling of N. cadamba grew well under 16-h light/ 8-h dark at room temperature. The good callogenic response was found in a culture that exposed to the 24-h dark treatment (88.9%). However, the 24-h light treatment resulted in a poor callogenic response (22.2%). The good callogenic response was found in B5 medium supplemented with a low concentration of auxins and cytokinins which was 1.0 mg/L of BAP (86.7%) and NAA (80.0%) within eight weeks of inoculation. Indirect shoot regeneration from calli derived from the young leaf explants was successfully initiated by inoculating callus onto B5 medium fortified with combinations of 2.0 mg/L BAP and 0.5 mg/L NAA. BAP 2.0 mg/L added with 10% coconut water also can help to promote cell regeneration, division and differentiation of meristem cell. N. cadamba shoots were actively regenerated when the nodal segments were inoculated on medium added with 1.0 mg/L BAP and 10% CW. About 97.8% of nodal segments were able to regenerate shoots, and an average of 19 shoots per explant was produced after 16 weeks of culture. The present study has successfully established the tissue culture protocol for propagating N. cadamba. This newly established protocol could pave the way to overcome the shortage of planting materials for planted forest development in Sarawak, which in turn assures the local timber industries to meet the global demand for wood-related products. Keywords: Neolamarckia cadamba, complex additives, in vitro seed germination, seedling growth, callus induction, indirect and direct shoot regeneration, plantlet regeneration.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Maslini, Japar Ali
author_facet	Maslini, Japar Ali
author_sort	Maslini, Japar Ali
title	Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
title_short	Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
title_full	Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
title_fullStr	Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
title_full_unstemmed	Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser
title_sort	tissue culture of neolamarckia cadamba (roxb.) bosser
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2020
url	http://ir.unimas.my/id/eprint/31419/2/Tissue%20Culture%20of%20Neolamarckia%20cadamba%20%28Roxb.%29%20Bosser.pdf
_version_	1783728401397317632

Tissue Culture of Neolamarckia cadamba (Roxb.) Bosser

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