Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM

The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the dir...

Full description

Saved in:
Bibliographic Details
Main Author: Bay, Hui Han
Format: Thesis
Language:English
Published: 2008
Subjects:
Online Access:http://ir.unimas.my/id/eprint/3588/1/Bay%20Hui%20Han%20ft.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-unimas-ir.3588
record_format uketd_dc
spelling my-unimas-ir.35882023-04-13T03:59:19Z Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM 2008 Bay, Hui Han QK Botany The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway. Universiti Malaysia Sarawak, (UNIMAS) 2008 Thesis http://ir.unimas.my/id/eprint/3588/ http://ir.unimas.my/id/eprint/3588/1/Bay%20Hui%20Han%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Institute of Biodiversity and Environmental Conservation.
institution Universiti Malaysia Sarawak
collection UNIMAS Institutional Repository
language English
topic QK Botany
spellingShingle QK Botany
Bay, Hui Han
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
description The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway.
format Thesis
qualification_level Master's degree
author Bay, Hui Han
author_facet Bay, Hui Han
author_sort Bay, Hui Han
title Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_short Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_full Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_fullStr Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_full_unstemmed Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_sort micropropagation and monitoring of genetic stability of micropagated plantlets of hornstedtia reticulata k. schum
granting_institution Universiti Malaysia Sarawak (UNIMAS)
granting_department Institute of Biodiversity and Environmental Conservation.
publishDate 2008
url http://ir.unimas.my/id/eprint/3588/1/Bay%20Hui%20Han%20ft.pdf
_version_ 1783727935373443072