Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status

The current study focused on establishment of mangrove based natural mud crab hatchery and assessment of different doses of dietary Lacto-sacc on mud crabs in the hatchery. The mated female crab samples were collected from the Sundarbans region and randomly divided into T1 (0 % Lacto-sacc), T2 (1 %...

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Main Author:	Md. Abdul, Hannan
Format:	Thesis
Language:	English
Published:	2024
Subjects:	SH Aquaculture Fisheries Angling
Online Access:	http://ir.unimas.my/id/eprint/46426/1/PhD%20Thesis_MA%20Hannan_%20FRST_UNIMAS-CGS.pdf
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id	my-unimas-ir.46426
record_format	uketd_dc
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SH Aquaculture Fisheries Angling
spellingShingle	SH Aquaculture Fisheries Angling Md. Abdul, Hannan Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
description	The current study focused on establishment of mangrove based natural mud crab hatchery and assessment of different doses of dietary Lacto-sacc on mud crabs in the hatchery. The mated female crab samples were collected from the Sundarbans region and randomly divided into T1 (0 % Lacto-sacc), T2 (1 % Lacto-sacc), and T3 (1.5 % Lacto-sacc) treatments. The crabs were identified as Scylla olivacea species through morphometric and molecular techniques. The heavy metals toxicity was determined and observed highest quantity of heavy metals in soil sediments followed by water and crabs. The Lacto-sacc diet was prepared manually contains 45 % protein, 12 % lipid, 40 % carbohydrate, 3 % ash and live microorganisms Lactobacillus acidophilus (1.2×108 cfu/g), Enterococcus faecium (7.3×107 cfu/g), and Saccharomyces cerevisiae (2.7×109 cells/g). The highest survival was obtained 10.53±1.56 a % in first trail at 26 ‰ and increased to 12.15±1.58 a % after application of 1.5% Lacto-sacc (T3). Breeding activities was performed in controlled condition and zoea were transferred to mangrove pens. The rotifer (Brachionus plicatilis) and Artemia franciscana were used to feed the zoea whereas the rotifer enriched with the Tetraselmis suecica, Nannochloropsis oculate and S. cerevisiae. The metamorphosis stages of zoea were passed in the mangrove pens and turned up to crablets (0.027±0.005 g) stages after 28 days. The cannibalism % of crablets was observed 71.20±2.08 tested after crossing the crablet stages. The immune parameters of Lacto-sacc challenged mud crabs were tested at 0, 4, 8, and 12 weeks of sampling. After 12 weeks the total hemocyte count was increased in T3 treatment that was 30.17 % higher than control (T1). The hemolymph clotting time and superoxide dismutase (SOD) enzymes activities were also increased in T3 treatment but not significantly different among the treatments. The enzyme phenoloxidase (PO) and prophenoloxidase (proPO) were increased in T3 treatment that was significantly different (P<0.05) among the treatments. The value of PO was 0.022±0.006b, 0.076±0.077b, 0.165±0.023a (unit/g) and the value of proPO was 0.031±0.032b, 0.040±0.016b, 0.121±0.014a (unit/g) for T1, T2 and T3 respectively. The challenge test of hatchery produced crablets was performed in In Vivo condition in which 1.5% Lacto-sacc enriched crablets (T3) obtained highest survival (61.67±2.89a %). Moreover, the digestive enzyme activity was observed 3581.09±758.69b, 2762.67±582.76ab, 6090.67±2386.91a unit/g for protease, and 249.33±103.87b, 453.33±207.74ab, 634.67±103.87a unit/g for lipase for the treatment T1, T2, and T3 respectively whereas no significant difference was observed for amylase enzyme. In addition, the transverse section of the mud crab hepatopancreas and gut indicates a significant difference among the treatments in terms of their structure and cells count. The analysis of next generation sequencing of Lacto-sacc challenged mud crab also implies the higher number of microbes in T3 followed by T2 and T1 treatments. Finally, it’s concluded that 1.5 % Lacto-sacc (T3) performed best which increases the strong immunity of brood crabs and crablets to fight against disease infestation and increases the higher survival rates.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Md. Abdul, Hannan
author_facet	Md. Abdul, Hannan
author_sort	Md. Abdul, Hannan
title	Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
title_short	Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
title_full	Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
title_fullStr	Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
title_full_unstemmed	Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status
title_sort	establishment of natural mangrove mud crab hatchery in bangladesh, and its effectiveness with dietary lacto-sacc (immunostimulant) on mud crab reproduction, nutritional digestibility, and health status
granting_institution	Elsevier Ltd.
granting_department	Comparative Immunology Reports
publishDate	2024
url	http://ir.unimas.my/id/eprint/46426/1/PhD%20Thesis_MA%20Hannan_%20FRST_UNIMAS-CGS.pdf
_version_	1818611698520555520
spelling	my-unimas-ir.464262024-10-21T02:30:24Z Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status 2024-04-09 Md. Abdul, Hannan SH Aquaculture. Fisheries. Angling The current study focused on establishment of mangrove based natural mud crab hatchery and assessment of different doses of dietary Lacto-sacc on mud crabs in the hatchery. The mated female crab samples were collected from the Sundarbans region and randomly divided into T1 (0 % Lacto-sacc), T2 (1 % Lacto-sacc), and T3 (1.5 % Lacto-sacc) treatments. The crabs were identified as Scylla olivacea species through morphometric and molecular techniques. The heavy metals toxicity was determined and observed highest quantity of heavy metals in soil sediments followed by water and crabs. The Lacto-sacc diet was prepared manually contains 45 % protein, 12 % lipid, 40 % carbohydrate, 3 % ash and live microorganisms Lactobacillus acidophilus (1.2×108 cfu/g), Enterococcus faecium (7.3×107 cfu/g), and Saccharomyces cerevisiae (2.7×109 cells/g). The highest survival was obtained 10.53±1.56 a % in first trail at 26 ‰ and increased to 12.15±1.58 a % after application of 1.5% Lacto-sacc (T3). Breeding activities was performed in controlled condition and zoea were transferred to mangrove pens. The rotifer (Brachionus plicatilis) and Artemia franciscana were used to feed the zoea whereas the rotifer enriched with the Tetraselmis suecica, Nannochloropsis oculate and S. cerevisiae. The metamorphosis stages of zoea were passed in the mangrove pens and turned up to crablets (0.027±0.005 g) stages after 28 days. The cannibalism % of crablets was observed 71.20±2.08 tested after crossing the crablet stages. The immune parameters of Lacto-sacc challenged mud crabs were tested at 0, 4, 8, and 12 weeks of sampling. After 12 weeks the total hemocyte count was increased in T3 treatment that was 30.17 % higher than control (T1). The hemolymph clotting time and superoxide dismutase (SOD) enzymes activities were also increased in T3 treatment but not significantly different among the treatments. The enzyme phenoloxidase (PO) and prophenoloxidase (proPO) were increased in T3 treatment that was significantly different (P<0.05) among the treatments. The value of PO was 0.022±0.006b, 0.076±0.077b, 0.165±0.023a (unit/g) and the value of proPO was 0.031±0.032b, 0.040±0.016b, 0.121±0.014a (unit/g) for T1, T2 and T3 respectively. The challenge test of hatchery produced crablets was performed in In Vivo condition in which 1.5% Lacto-sacc enriched crablets (T3) obtained highest survival (61.67±2.89a %). Moreover, the digestive enzyme activity was observed 3581.09±758.69b, 2762.67±582.76ab, 6090.67±2386.91a unit/g for protease, and 249.33±103.87b, 453.33±207.74ab, 634.67±103.87a unit/g for lipase for the treatment T1, T2, and T3 respectively whereas no significant difference was observed for amylase enzyme. In addition, the transverse section of the mud crab hepatopancreas and gut indicates a significant difference among the treatments in terms of their structure and cells count. The analysis of next generation sequencing of Lacto-sacc challenged mud crab also implies the higher number of microbes in T3 followed by T2 and T1 treatments. Finally, it’s concluded that 1.5 % Lacto-sacc (T3) performed best which increases the strong immunity of brood crabs and crablets to fight against disease infestation and increases the higher survival rates. Elsevier Ltd. 2024-04 Thesis http://ir.unimas.my/id/eprint/46426/ http://ir.unimas.my/id/eprint/46426/1/PhD%20Thesis_MA%20Hannan_%20FRST_UNIMAS-CGS.pdf text en public https://doi.org/10.1016/j.cirep.2024.200156 phd doctoral Elsevier Ltd. Comparative Immunology Reports UNDP Hannan, M. A., Pandit, C., Hasan, M. J., Mahdi, G. M. A., Asdari, R., & Munir, M. B. (2024). Quantification of heavy metals in wild gravid female mud crab (Scylla olivacea) collected from different rivers of Sundarbans mangrove region. Desalination and Water Treatment, 319, 100521. Hannan, M. A., Munir, M. B., Jamy, M., Habib, K. A., Hasan, M. J., Asdari, R., Nondi, S., Eissa E.H. & Eissa, M. E. (2024). Natural breeding performance in different saline water pens, crablets rearing and cannibalism in juvenile phase of mangrove mud crab (Scylla olivacea) in earthen mangrove pens. Desalination and Water Treatment, 100500. Hannan, M. A., Munir, M. B., Asdari, R., Islam, M. S., Lima, R. A., Islam, H. R., Rashid, M. H. & Hing, H. W. Y. (2024). Dietary Lacto-sacc stimulates the immune response of gravid mud crab (Scylla olivacea). Comparative Immunology Reports, 7, 200156.

Establishment of Natural Mangrove Mud Crab Hatchery in Bangladesh, and Its Effectiveness with Dietary Lacto-sacc (Immunostimulant) on Mud Crab Reproduction, Nutritional Digestibility, and Health Status

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