Bacterial inoculum development for effluents treatment of shrimp farming industry

In shrimp-pond farming, effective water quality management is critical for shrimp growth and survival as well as to reduce the impact of shrimp farming on the environment. Therefore, this study was conducted with the objective of developing indigenous bacterial formulation, which can effectively tre...

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Main Author:	Evayantie Wahyuni, Binti Zamudin
Format:	Thesis
Language:	English
Published:	2013
Subjects:	SH Aquaculture Fisheries Angling
Online Access:	http://ir.unimas.my/id/eprint/9350/1/Evayantie%20Wahyuni%20Binti%20Zamudin%20ft.pdf
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spelling	my-unimas-ir.93502023-06-12T03:16:18Z Bacterial inoculum development for effluents treatment of shrimp farming industry 2013 Evayantie Wahyuni, Binti Zamudin SH Aquaculture. Fisheries. Angling In shrimp-pond farming, effective water quality management is critical for shrimp growth and survival as well as to reduce the impact of shrimp farming on the environment. Therefore, this study was conducted with the objective of developing indigenous bacterial formulation, which can effectively treat shrimp pond effluents thus improving the water quality in shrimp farming. To ascertain possible bacterial species to be used in the formulation, water samples were collected from two shrimp farms in Muara Tebas and Santubong, Kuching, Sarawak, Malaysia. Eleven water samples were collected from these two shrimp farms. Microorganisms in the water samples with the necessary characteristics such as active proteolytic, cellulolytic and amylolytic were isolated. These eight species of microorganisms were then identified based on biochemical test, API 20NE and API 20E kit, followed by Polymerase Chain Reaction (PCR) technique. The main species that had been identified as potential degraders of pond effluents belonged to the Bacillus sp.. From the primary screening on solid media, eight active isolates were chosen based on the size of the diameter of the clear zone surrounding the colonies in the plate-screening medium. A time course study was performed for 72 hours for each of the potential isolates in liquid media to analyze their growth curve and enzymes production with respect to time. These isolates were individually further grown in shaked flask cultures to determine the pattern of enzyme production, protein content and bacterial growth. All of the monocultures (eight isolates) were then combined in minimal media for 24 hours at 37oC to determine the pattern of growth and enzymes production when in combination of 1 : 1, 1 : 2, 2 : 1, 1 : 3, 3 : 1, 3 : 2 and 2 : 3. From the results obtained from biculture combination, the best combination of bacteria was then tested for their ability to grow and respond in a triculture system. 1% of Bacillus megaterium (S120): Bacillus cereus strain 3 (S65): Enterococcus casseliflavus (S11) was selected as the best triculture combination because they produced the highest enzymes of cellulase, amylase and protease. Therefore, the formulation consists of three bacterial species identified as Bacillus megaterium, Bacillus cereus and Enterococcus casseliflavus. The bacterial formulation was then tested on shrimp pond water from two different aquaculture farms and UNIMAS’s pond water. Chemical and biological parameters in the water were analyzed for seven days except for UNIMAS pond where the treatment was extended to fourteen days. There were no significant differences (P > 0.05) in water quality parameters such as orthophosphate, total phosphorus, bacterial count, temperature and pH for samples from the shrimp pond water. However, ammonia-nitrogen, nitrite, nitrate, turbidity, suspended solid, chemical oxygen demand (COD), sulfide and biochemical oxygen demand (BOD) differed significantly (P < 0.05). Meanwhile for water samples from UNIMAS pond, nitrate, bacterial count, temperature and pH showed no significant differences (P > 0.05). Other parameters such as ammonia-nitrogen, nitrite, orthophosphate, total phosphorus, turbidity, suspended solid, chemical oxygen demand (COD), sulfide and biochemical oxygen demand (BOD) differed significantly (P < 0.05) compared to control experiment. The ability to reduce more than 50% of eight water quality parameters in this study clearly showed that the indigenous bacterial inoculums were a good shrimp pond effluents degrader. University Malaysia Sarawak, UNIMAS 2013 Thesis http://ir.unimas.my/id/eprint/9350/ http://ir.unimas.my/id/eprint/9350/1/Evayantie%20Wahyuni%20Binti%20Zamudin%20ft.pdf text en validuser masters University Malaysia Sarawak, UNIMAS Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SH Aquaculture Fisheries Angling
spellingShingle	SH Aquaculture Fisheries Angling Evayantie Wahyuni, Binti Zamudin Bacterial inoculum development for effluents treatment of shrimp farming industry
description	In shrimp-pond farming, effective water quality management is critical for shrimp growth and survival as well as to reduce the impact of shrimp farming on the environment. Therefore, this study was conducted with the objective of developing indigenous bacterial formulation, which can effectively treat shrimp pond effluents thus improving the water quality in shrimp farming. To ascertain possible bacterial species to be used in the formulation, water samples were collected from two shrimp farms in Muara Tebas and Santubong, Kuching, Sarawak, Malaysia. Eleven water samples were collected from these two shrimp farms. Microorganisms in the water samples with the necessary characteristics such as active proteolytic, cellulolytic and amylolytic were isolated. These eight species of microorganisms were then identified based on biochemical test, API 20NE and API 20E kit, followed by Polymerase Chain Reaction (PCR) technique. The main species that had been identified as potential degraders of pond effluents belonged to the Bacillus sp.. From the primary screening on solid media, eight active isolates were chosen based on the size of the diameter of the clear zone surrounding the colonies in the plate-screening medium. A time course study was performed for 72 hours for each of the potential isolates in liquid media to analyze their growth curve and enzymes production with respect to time. These isolates were individually further grown in shaked flask cultures to determine the pattern of enzyme production, protein content and bacterial growth. All of the monocultures (eight isolates) were then combined in minimal media for 24 hours at 37oC to determine the pattern of growth and enzymes production when in combination of 1 : 1, 1 : 2, 2 : 1, 1 : 3, 3 : 1, 3 : 2 and 2 : 3. From the results obtained from biculture combination, the best combination of bacteria was then tested for their ability to grow and respond in a triculture system. 1% of Bacillus megaterium (S120): Bacillus cereus strain 3 (S65): Enterococcus casseliflavus (S11) was selected as the best triculture combination because they produced the highest enzymes of cellulase, amylase and protease. Therefore, the formulation consists of three bacterial species identified as Bacillus megaterium, Bacillus cereus and Enterococcus casseliflavus. The bacterial formulation was then tested on shrimp pond water from two different aquaculture farms and UNIMAS’s pond water. Chemical and biological parameters in the water were analyzed for seven days except for UNIMAS pond where the treatment was extended to fourteen days. There were no significant differences (P > 0.05) in water quality parameters such as orthophosphate, total phosphorus, bacterial count, temperature and pH for samples from the shrimp pond water. However, ammonia-nitrogen, nitrite, nitrate, turbidity, suspended solid, chemical oxygen demand (COD), sulfide and biochemical oxygen demand (BOD) differed significantly (P < 0.05). Meanwhile for water samples from UNIMAS pond, nitrate, bacterial count, temperature and pH showed no significant differences (P > 0.05). Other parameters such as ammonia-nitrogen, nitrite, orthophosphate, total phosphorus, turbidity, suspended solid, chemical oxygen demand (COD), sulfide and biochemical oxygen demand (BOD) differed significantly (P < 0.05) compared to control experiment. The ability to reduce more than 50% of eight water quality parameters in this study clearly showed that the indigenous bacterial inoculums were a good shrimp pond effluents degrader.
format	Thesis
qualification_level	Master's degree
author	Evayantie Wahyuni, Binti Zamudin
author_facet	Evayantie Wahyuni, Binti Zamudin
author_sort	Evayantie Wahyuni, Binti Zamudin
title	Bacterial inoculum development for effluents treatment of shrimp farming industry
title_short	Bacterial inoculum development for effluents treatment of shrimp farming industry
title_full	Bacterial inoculum development for effluents treatment of shrimp farming industry
title_fullStr	Bacterial inoculum development for effluents treatment of shrimp farming industry
title_full_unstemmed	Bacterial inoculum development for effluents treatment of shrimp farming industry
title_sort	bacterial inoculum development for effluents treatment of shrimp farming industry
granting_institution	University Malaysia Sarawak, UNIMAS
granting_department	Faculty of Resource Science and Technology
publishDate	2013
url	http://ir.unimas.my/id/eprint/9350/1/Evayantie%20Wahyuni%20Binti%20Zamudin%20ft.pdf
_version_	1783728050771329024

Bacterial inoculum development for effluents treatment of shrimp farming industry

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