Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)

Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in Sarawak. The potential use as starch resource for the food industry required an increase its production. Under certain conditions, some sago palms do not develop well and become stunted. This is considered as...

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Main Author:	Siti Izyan Liyana, Kamarol
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QK Botany SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/9389/1/Siti%20Izyan.pdf
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id	my-unimas-ir.9389
record_format	uketd_dc
spelling	my-unimas-ir.93892023-08-01T06:27:47Z Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu) 2015 Siti Izyan Liyana, Kamarol QK Botany SB Plant culture Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in Sarawak. The potential use as starch resource for the food industry required an increase its production. Under certain conditions, some sago palms do not develop well and become stunted. This is considered as non-trunking sago palm. Morphologically, this type of sago palm is not normal and this led to wastage of various resources and affects the starch production. The research objective was to understand the molecular process that affect non-trunking and starch synthesis in sago palm. Representational Difference Analysis was performed to identify the differences between trunking and non-trunking sago palm transcriptome. This subtractive hybridization technique detects the differences in gene expression. After three rounds of hybridization, the sequences obtained were compared with the sequences in NCBI database. Results showed the activated genes in response to the non trunking sago palm have similarity to genes that are implicated in plant growth, cell metabolism and salt or osmotic stress. The differential expressed genes in trunking sago palm code for genes related to growth development and signalling in neutralizing the imbalance in plant stress. The information obtained through this work can be used to develop database related to trunking and non-trunking sago palm. Universiti Malaysia Sarawak, (UNIMAS) 2015 Thesis http://ir.unimas.my/id/eprint/9389/ http://ir.unimas.my/id/eprint/9389/1/Siti%20Izyan.pdf text en validuser masters Universiti Malaysia Sarawak, (UNIMAS) Faculty of Resource Science and Technology.
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture Siti Izyan Liyana, Kamarol Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
description	Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in Sarawak. The potential use as starch resource for the food industry required an increase its production. Under certain conditions, some sago palms do not develop well and become stunted. This is considered as non-trunking sago palm. Morphologically, this type of sago palm is not normal and this led to wastage of various resources and affects the starch production. The research objective was to understand the molecular process that affect non-trunking and starch synthesis in sago palm. Representational Difference Analysis was performed to identify the differences between trunking and non-trunking sago palm transcriptome. This subtractive hybridization technique detects the differences in gene expression. After three rounds of hybridization, the sequences obtained were compared with the sequences in NCBI database. Results showed the activated genes in response to the non trunking sago palm have similarity to genes that are implicated in plant growth, cell metabolism and salt or osmotic stress. The differential expressed genes in trunking sago palm code for genes related to growth development and signalling in neutralizing the imbalance in plant stress. The information obtained through this work can be used to develop database related to trunking and non-trunking sago palm.
format	Thesis
qualification_level	Master's degree
author	Siti Izyan Liyana, Kamarol
author_facet	Siti Izyan Liyana, Kamarol
author_sort	Siti Izyan Liyana, Kamarol
title	Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
title_short	Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
title_full	Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
title_fullStr	Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
title_full_unstemmed	Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
title_sort	representational difference analysis (rda) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
granting_institution	Universiti Malaysia Sarawak, (UNIMAS)
granting_department	Faculty of Resource Science and Technology.
publishDate	2015
url	http://ir.unimas.my/id/eprint/9389/1/Siti%20Izyan.pdf
_version_	1783728054816735232

Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)

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