Germplasm Preservation: Storage of Seed, Pollen and Tissue of Selected Malaysian Orchid Species
In an attempt to develop methods for cheap, long-term preservation of orchid germplasm, seed, pollen and tissue of four local species were subjected to various storage conditions and subsequently determined of their effects on viability. The resultant quality of stored materials varied over the...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1987
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10219/1/FP_1987_1.pdf |
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| Summary: | In an attempt to develop methods for cheap, long-term
preservation of orchid germplasm, seed, pollen and tissue of
four local species were subjected to various storage conditions
and subsequently determined of their effects on viability. The
resultant quality of stored materials varied over the range of
conditions and species investigated.
Storage of seeds of Arundina graminifolia, Cymbidium
finlaysonianum and Spathoglottis plicata at seed moisture
content of less than 5 percent and at storage temperature of
less than 5°C gave percentage viability that ranged between 60
and 90 percent for a storage duration of more than twelve
weeks. Storage in liquid nitrogen (-196°C) gave the highest mean among the temperatures tested, confirming the orthodox
nature of the seed. |
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