Studies on Corynespora Cassiicola (Berk. & Curt.) Wei on Rubber
Corynespora cassiicola (Berk. & Curt.) Wei from infected rubber leaves in Sungai Buloh (two isolates) and Serdang (two isolates) were cultured on Potato Dextrose Agar (PDA). All four isolates exhibited variability in colony colour and growth rates on artificial media (PDA, PSA, RLEA and WA)....
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| Format: | Thesis |
| Language: | English English |
| Published: |
1995
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10352/1/FP_1995_1_A.pdf |
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| Summary: | Corynespora cassiicola (Berk. & Curt.) Wei from infected rubber
leaves in Sungai Buloh (two isolates) and Serdang (two isolates) were cultured
on Potato Dextrose Agar (PDA). All four isolates exhibited variability in
colony colour and growth rates on artificial media (PDA, PSA, RLEA and
WA). The optimum temperature for growth on PDA was 28°C.
Significant increase in sporulation was recorded on Potato Sucrose
Agar (PSA) when exposed to uv light for 2 h daily for three days. Exposure to nuv light also increased sporulation significantly but maximum sporulation
occurred only with 4 h daily treatment.
Conidial size, hilum width and septal number of isolates cultured on
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PSA were variable and were not affected by uv light treatment. The size and
shape of conidia on PSA differed from those on naturally infected rubber
leaves. On rubber leaves, the average conidial and hilum width were longer,
and the average septal number was higher. Conidia were long, slender and
stick-like in culture, but were curved, with a broader based on rubber leaves.
Maximulll sporulation was achieved when cultures on PSA were
scraped once, and maintained for three days under continuous fluorescent
light with 2 h exposure to uv light daily. By this method, variation of uv light
exposure of between 15 mi n to 4 h daily was found to have no negative effect
on conidial germination and germ tube growth. PSA was the best medium for
sporulation compared to PDA and RLEA. The addition of yeast extract in
these media did not improve sporulation. |
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