Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation

HIV is a global health concern, including in Malaysia, where more than a hundred thousand cases has been reported since 1986 until 2016. After the introduction of highly active antiretroviral therapy (HAART) regimen, HIV- related mortality and morbidity has greatly declined. Despite that, HIV clinic...

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Main Author:	Achim, Nurfarahin Hanini
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Beta - Defensins HIV
Online Access:	http://psasir.upm.edu.my/id/eprint/103842/1/NURFARAHIN%20HANINI%20BINTI%20-%20IR.pdf
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spelling	my-upm-ir.1038422023-05-22T01:24:38Z Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation 2020-01 Achim, Nurfarahin Hanini HIV is a global health concern, including in Malaysia, where more than a hundred thousand cases has been reported since 1986 until 2016. After the introduction of highly active antiretroviral therapy (HAART) regimen, HIV- related mortality and morbidity has greatly declined. Despite that, HIV clinical course were identified to be variable across population. Host genetic variation has been long recognized to have a part in influencing the susceptibility and prognosis of HIV infection. Beta-defensins gene (DEFB) encoded beta-defensins peptide, which has anti-HIV and chemoattractant properties. Interestingly, DEFB is characterized as copy number variables (CNV), a structural variation that presents in variable copy number between individuals. CNV may alter the expression of the gene and subsequently, will affect the susceptibility and progression of diseases. The CNV of DEFB has been associated with susceptibility towards a few diseases, including HIV. So far, there is still lack in information on distribution of DEFB copy number, especially among Malaysian HIV-infected patients. Moreover, the investigation on the association of DEFB CNV with CD4 count and viral load of Malaysian HIV patients are also lacking. Therefore, this study aimed to investigate the relationship of DEFB gene copy number variability with CD4 count and viral load in Malaysian HIV patients. A total of 182 HIV-infected patients and 156 controls were recruited in this study. DEFB copy number of all the participants was quantified using Triplex PRTs and validated with microsatellite analysis. DEFB copy number in HIV patients were found from 2 and 8 copies, and the modal copy number was 4. No significant difference was identified in DEFB distribution among Malay, Chinese, and Indian HIV patients. A comparison between HIV and control group found that individuals with a high copy number (> 4) are significantly higher among HIV patients as compared to controls (p = 0.039). However, no significant association was identified between DEFB copy number with the recovery of CD4 count to a normal level and viral load suppression. In conclusion, while individuals with high DEFB copy number is suggested to have higher susceptibility towards HIV, DEFB is not a significant factor in influencing the disease prognosis. Beta - Defensins Antiretroviral Therapy, Highly Active HIV 2020-01 Thesis http://psasir.upm.edu.my/id/eprint/103842/ http://psasir.upm.edu.my/id/eprint/103842/1/NURFARAHIN%20HANINI%20BINTI%20-%20IR.pdf text en public masters Universiti Putra Malaysia Beta - Defensins Antiretroviral Therapy, Highly Active HIV Abu Bakar @ Jamaludin, Suhaili
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Abu Bakar @ Jamaludin, Suhaili
topic	Beta - Defensins Beta - Defensins HIV
spellingShingle	Beta - Defensins Beta - Defensins HIV Achim, Nurfarahin Hanini Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
description	HIV is a global health concern, including in Malaysia, where more than a hundred thousand cases has been reported since 1986 until 2016. After the introduction of highly active antiretroviral therapy (HAART) regimen, HIV- related mortality and morbidity has greatly declined. Despite that, HIV clinical course were identified to be variable across population. Host genetic variation has been long recognized to have a part in influencing the susceptibility and prognosis of HIV infection. Beta-defensins gene (DEFB) encoded beta-defensins peptide, which has anti-HIV and chemoattractant properties. Interestingly, DEFB is characterized as copy number variables (CNV), a structural variation that presents in variable copy number between individuals. CNV may alter the expression of the gene and subsequently, will affect the susceptibility and progression of diseases. The CNV of DEFB has been associated with susceptibility towards a few diseases, including HIV. So far, there is still lack in information on distribution of DEFB copy number, especially among Malaysian HIV-infected patients. Moreover, the investigation on the association of DEFB CNV with CD4 count and viral load of Malaysian HIV patients are also lacking. Therefore, this study aimed to investigate the relationship of DEFB gene copy number variability with CD4 count and viral load in Malaysian HIV patients. A total of 182 HIV-infected patients and 156 controls were recruited in this study. DEFB copy number of all the participants was quantified using Triplex PRTs and validated with microsatellite analysis. DEFB copy number in HIV patients were found from 2 and 8 copies, and the modal copy number was 4. No significant difference was identified in DEFB distribution among Malay, Chinese, and Indian HIV patients. A comparison between HIV and control group found that individuals with a high copy number (> 4) are significantly higher among HIV patients as compared to controls (p = 0.039). However, no significant association was identified between DEFB copy number with the recovery of CD4 count to a normal level and viral load suppression. In conclusion, while individuals with high DEFB copy number is suggested to have higher susceptibility towards HIV, DEFB is not a significant factor in influencing the disease prognosis.
format	Thesis
qualification_level	Master's degree
author	Achim, Nurfarahin Hanini
author_facet	Achim, Nurfarahin Hanini
author_sort	Achim, Nurfarahin Hanini
title	Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
title_short	Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
title_full	Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
title_fullStr	Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
title_full_unstemmed	Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation
title_sort	association of beta-defensin gene copy number variation with cd4 count and viral load among malaysian ethnics hiv patients after haart initiation
granting_institution	Universiti Putra Malaysia
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/103842/1/NURFARAHIN%20HANINI%20BINTI%20-%20IR.pdf
_version_	1776100373420834816

Association of beta-defensin gene copy number variation with CD4 count and viral load among Malaysian ethnics hiv patients after HAART initiation

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