Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique
The present study evaluates the effects of various loading solutions, concentrations of glycerol and vitrification solutions and their time of exposure on the vitrification of rambutan embryos in liquid nitrogen. In the initial study to evaluate the effects of loading solutions on survival, exc...
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my-upm-ir.105622024-04-17T03:22:14Z Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique 2001-02 Camillus Ginibun, Florence The present study evaluates the effects of various loading solutions, concentrations of glycerol and vitrification solutions and their time of exposure on the vitrification of rambutan embryos in liquid nitrogen. In the initial study to evaluate the effects of loading solutions on survival, excised embryos were exposed to four loading solutions. The two most promising loading solutions were LB (1.5 M glycerol + 0.4 M sucrose + 5% DMSO), which gave 44.0 % viability and 32.4 % survival and LA (2.0 M glycerol + 0.4 M sucrose) which gave 39.3 % viability and 28.1 % survival after freezing. The effects of different concentrations of glycerol (0 - 2.0 M) in the most promising loading solutions were evaluated further. For loading solution LB, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LS, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LA, 0 M glycerol or the use of only 0.4 M sucrose gave the highest viability of 76.0 % and survival of 59.0 %. Hence, loading solution with only 0.4 M sucrose (LA without glycerol) was established in this study as the most effective loading solution for rambutan embryos. The effects of exposure time (0-16 hours) to the best loading solution on survival of rambutan embryos were further investigated. It was found that 8 hours duration gave the highest viability (47.7 %) and survival (32.8 %). Having confirmed the best loading treatment, the study further evaluates the effects of six vitrification solutions on survival of rambutan embryos in liquid nitrogen. The results show that after freezing, L Solution gave the highest viability (46.0 %) and survival (24.0 %). L Solution was therefore selected as the most effective vitrification solution. In optimizing the time of exposure, excised rambutan embryos were exposed to L Solution for 0 to 90 minutes before LN exposure. The highest viability (55.6 %) and survival (40.3 %) after vitirification were achieved at 60 minutes exposure. Longer exposure to L Solution for up to 90 minutes reduced survival to 16.0 %. Rambutan - Case studies 2001-02 Thesis http://psasir.upm.edu.my/id/eprint/10562/ http://psasir.upm.edu.my/id/eprint/10562/1/FP_2001_18.pdf text en public masters Universiti Putra Malaysia Rambutan - Case studies Faculty of Agriculture Hor, Yue Luan English |
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English English |
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Hor, Yue Luan |
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Rambutan - Case studies |
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Rambutan - Case studies Camillus Ginibun, Florence Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
description |
The present study evaluates the effects of various loading
solutions, concentrations of glycerol and vitrification solutions and their
time of exposure on the vitrification of rambutan embryos in liquid
nitrogen.
In the initial study to evaluate the effects of loading solutions on
survival, excised embryos were exposed to four loading solutions. The
two most promising loading solutions were LB (1.5 M glycerol + 0.4 M
sucrose + 5% DMSO), which gave 44.0 % viability and 32.4 % survival
and LA (2.0 M glycerol + 0.4 M sucrose) which gave 39.3 % viability and
28.1 % survival after freezing.
The effects of different concentrations of glycerol (0 - 2.0 M) in
the most promising loading solutions were evaluated further. For loading
solution LB, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LS, 1.5 M glycerol gave highest survival of 22.7 %. For loading
solution LA, 0 M glycerol or the use of only 0.4 M sucrose gave the
highest viability of 76.0 % and survival of 59.0 %. Hence, loading
solution with only 0.4 M sucrose (LA without glycerol) was established in
this study as the most effective loading solution for rambutan embryos.
The effects of exposure time (0-16 hours) to the best loading
solution on survival of rambutan embryos were further investigated. It
was found that 8 hours duration gave the highest viability (47.7 %) and
survival (32.8 %).
Having confirmed the best loading treatment, the study further
evaluates the effects of six vitrification solutions on survival of rambutan
embryos in liquid nitrogen. The results show that after freezing,
L Solution gave the highest viability (46.0 %) and survival (24.0 %).
L Solution was therefore selected as the most effective vitrification
solution.
In optimizing the time of exposure, excised rambutan embryos
were exposed to L Solution for 0 to 90 minutes before LN exposure. The
highest viability (55.6 %) and survival (40.3 %) after vitirification were
achieved at 60 minutes exposure. Longer exposure to L Solution for up
to 90 minutes reduced survival to 16.0 %. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Camillus Ginibun, Florence |
author_facet |
Camillus Ginibun, Florence |
author_sort |
Camillus Ginibun, Florence |
title |
Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
title_short |
Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
title_full |
Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
title_fullStr |
Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
title_full_unstemmed |
Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique |
title_sort |
cryopreservation of excised embryos of rambutan (nephelium lappaceum l.) using vitrification technique |
granting_institution |
Universiti Putra Malaysia |
granting_department |
Faculty of Agriculture |
publishDate |
2001 |
url |
http://psasir.upm.edu.my/id/eprint/10562/1/FP_2001_18.pdf |
_version_ |
1804888568081940480 |