Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
One of the major constraints in increasing crop yield is the supply of nutrient and nitrogen is obviously the main limiting nutrient. Biological nitrogen fixation is believed to have a great potential to contribute to productive and sustainable agricultural system for the tropics. Bacillus sphae...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2002
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10631/1/FP_2002_39.pdf |
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| Summary: | One of the major constraints in increasing crop yield is the supply of
nutrient and nitrogen is obviously the main limiting nutrient. Biological
nitrogen fixation is believed to have a great potential to contribute to
productive and sustainable agricultural system for the tropics. Bacillus
sphaericus UPMB10 is a locally isolated plant growth promoting
rhizobacteria that has the ability to fix atmospheric nitrogen efficiently and
has been proven to promote the growth of vegetable soybean, oil palm
and bananas. When UPMB10 is applied to the soil as a biofertilizer, it is
very important to have a method of identifying and monitoring the
effectiveness of the introduced inoculant. Polymerase chain reaction
based DNA fingerprinting, a DNA polymorphism assay based on the
amplification of random DNA segment with single primers of arbitrary
nucleotide sequence, was employed to detect UPMB10 used as an inoculant. DNA polymorphism simply detects DNA segments, which are
amplified from one individual bacterium but not others and the polymorph isms function as genetic markers. peR amplification does not
require the culturing of the bacterial strains and since it is capable of
amplifying unique sequences in the midst of a myriad of DNA sequences,
it has the potential to identify specific strains found within the soil. The
objectives of this study were to identify suitable primers for identification
and distinguishing UPMB1 0 when applied as a biofertilizer. |
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