Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique

One of the major constraints in increasing crop yield is the supply of nutrient and nitrogen is obviously the main limiting nutrient. Biological nitrogen fixation is believed to have a great potential to contribute to productive and sustainable agricultural system for the tropics. Bacillus sphae...

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Main Author:	Khor, Sock kun
Format:	Thesis
Language:	English English
Published:	2002
Subjects:	Bacillus sphaericus - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10631/1/FP_2002_39.pdf
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id	my-upm-ir.10631
record_format	uketd_dc
spelling	my-upm-ir.106312024-04-30T04:40:06Z Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique 2002-05 Khor, Sock kun One of the major constraints in increasing crop yield is the supply of nutrient and nitrogen is obviously the main limiting nutrient. Biological nitrogen fixation is believed to have a great potential to contribute to productive and sustainable agricultural system for the tropics. Bacillus sphaericus UPMB10 is a locally isolated plant growth promoting rhizobacteria that has the ability to fix atmospheric nitrogen efficiently and has been proven to promote the growth of vegetable soybean, oil palm and bananas. When UPMB10 is applied to the soil as a biofertilizer, it is very important to have a method of identifying and monitoring the effectiveness of the introduced inoculant. Polymerase chain reaction based DNA fingerprinting, a DNA polymorphism assay based on the amplification of random DNA segment with single primers of arbitrary nucleotide sequence, was employed to detect UPMB10 used as an inoculant. DNA polymorphism simply detects DNA segments, which are amplified from one individual bacterium but not others and the polymorph isms function as genetic markers. peR amplification does not require the culturing of the bacterial strains and since it is capable of amplifying unique sequences in the midst of a myriad of DNA sequences, it has the potential to identify specific strains found within the soil. The objectives of this study were to identify suitable primers for identification and distinguishing UPMB1 0 when applied as a biofertilizer. Bacillus sphaericus - Case studies 2002-05 Thesis http://psasir.upm.edu.my/id/eprint/10631/ http://psasir.upm.edu.my/id/eprint/10631/1/FP_2002_39.pdf text en public masters Universiti Putra Malaysia Bacillus sphaericus - Case studies Faculty of Agriculture Mohd Saud, Halimi English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Mohd Saud, Halimi
topic	Bacillus sphaericus - Case studies
spellingShingle	Bacillus sphaericus - Case studies Khor, Sock kun Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
description	One of the major constraints in increasing crop yield is the supply of nutrient and nitrogen is obviously the main limiting nutrient. Biological nitrogen fixation is believed to have a great potential to contribute to productive and sustainable agricultural system for the tropics. Bacillus sphaericus UPMB10 is a locally isolated plant growth promoting rhizobacteria that has the ability to fix atmospheric nitrogen efficiently and has been proven to promote the growth of vegetable soybean, oil palm and bananas. When UPMB10 is applied to the soil as a biofertilizer, it is very important to have a method of identifying and monitoring the effectiveness of the introduced inoculant. Polymerase chain reaction based DNA fingerprinting, a DNA polymorphism assay based on the amplification of random DNA segment with single primers of arbitrary nucleotide sequence, was employed to detect UPMB10 used as an inoculant. DNA polymorphism simply detects DNA segments, which are amplified from one individual bacterium but not others and the polymorph isms function as genetic markers. peR amplification does not require the culturing of the bacterial strains and since it is capable of amplifying unique sequences in the midst of a myriad of DNA sequences, it has the potential to identify specific strains found within the soil. The objectives of this study were to identify suitable primers for identification and distinguishing UPMB1 0 when applied as a biofertilizer.
format	Thesis
qualification_level	Master's degree
author	Khor, Sock kun
author_facet	Khor, Sock kun
author_sort	Khor, Sock kun
title	Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
title_short	Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
title_full	Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
title_fullStr	Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
title_full_unstemmed	Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique
title_sort	identification of a plant growth promoting rhizobacteria, bacillus sphaericus (upmb10), using pcr-based dna fingerprinting technique
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/10631/1/FP_2002_39.pdf
_version_	1804888581145100288

Identification of a Plant Growth Promoting Rhizobacteria, Bacillus Sphaericus (UPMB10), Using PCR-Based DNA Fingerprinting Technique

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