Induced Resistance Using Nonpathogenic Fusarium Oxysporum for Biological Control of Banana Fusarium Wilt
Banana (Musa spp. Linn.) is the second most important fruit crops in Malaysia. It is easily attacked by the fungus Fusarium oxysporum Schlect. fsp. cubense (E.F.Smith) Snyder and Hansen (FOC), causing terminal wilt in the field. Chemical and cultural methods were not effective in controlling the...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10682/1/FP_2003_32_.pdf |
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| Summary: | Banana (Musa spp. Linn.) is the second most important fruit crops in Malaysia. It is
easily attacked by the fungus Fusarium oxysporum Schlect. fsp. cubense (E.F.Smith)
Snyder and Hansen (FOC), causing terminal wilt in the field. Chemical and cultural
methods were not effective in controlling the disease. Therefore, alternative control
measures have to be developed. In recent years, nonpathogenic Fusaria was being
considered for plant disease control and could be the most promising approach for
biological control of Fusarium wilt in banana through induced systemic resistance.
Isolates of nonpathogenic F oxysporum (FO: F01, F02, F03, F04, F05 and F06)
were isolated from healthy roots and rhizospheres of bananas vars. Berangan and
Rastali, and were identified to the species level based on cultural and morphological
characteristics. Random amplified polymorphic (RAPD-PCR) analysis was able to establish variability within F. oxy.\porom isolates and between saprophytic and
pathogenic fonns (FOe race 1 and race 4), using ope 11 and ope 14 primers. All 6
isolates of FO were antagonistic to both pathogenic race 1 and race 4 of FOe with
values of the % of inhibition of radial growth (PIRG) exceeding 50% in a series of
dual culture test. F04 was found to be the most antagonistic against FOC' race 4 with
PIRGof65%.
Infectivity studies on six-weeks-old tissue cultured banana seedlings var. Berangan
cv. intan, con finned that FO1, F02, F03, F04, F05 or F06 were not pathogenic to
banana seedlings. No visible foliar or internal symptoms were observed both on
inoculated and control seedlings. Seedlings inoculated with FOC' race 4 produced
foliar symptoms as yellowing of the older leaves followed by necrosis and wilting.
F04 conferred some degree of resistance to the host when challenged with FOe race
I suggesting the possible role of induced resistance against Fusarium wilt. |
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