Carbon Flux Analysis Of Lipid Biosynthesis Pathways In Oil Palm (Elaeis Guineensis Jacq. Tenera)
The aim of this study was to investigate the carbon flux through lipid biosynthesis pathways in the oil palm (Elaeis guineensis Jacq. tenera) using metabolic control analysis (MCA). Three types of tissue from oil palm, namely liquid culture, plastid and mesocarp were used. The results showed meso...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2002
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11235/1/FPSK_M_2002_3.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The aim of this study was to investigate the carbon flux through lipid biosynthesis
pathways in the oil palm (Elaeis guineensis Jacq. tenera) using metabolic control
analysis (MCA). Three types of tissue from oil palm, namely liquid culture, plastid and
mesocarp were used. The results showed mesocarp tissue was the most suitable tissue
for carbon flux analysis because it incorporated the radioactive precursors mainly into
triacylglycerol (TAG). Further analysis on different stages of fruit development was
carried out using mesocarp tissue at 12, 1 5 and 20 weeks after anthesis (JV AA). It was
confirmed that 2 0-W AA mesocarp tissue was the best stage of fruit development for
metabolic flux studies because it reflected biosynthesis of storage lipid. Three modes of
radiolabel introduction into the oil palm fruits were investigated, namely injecting the
radiolabel into the fruits still attached to the palm, injecting the radiolabel into the loose
fruit and injecting the radio label into incubation mixture containing meso carp tissue
slices. The level of radioactivity in fruits attached to the palm was lower than the other
two modes of radiolabel introduction. Carbon flux of lipid b iosynthesis pathways was
modulated by temperature and the inhibitor 2-bromooctanoate. Radiolabels [ 1_14C] |
|---|