Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link

One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (natur...

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Main Author:	Long, Kamariah
Format:	Thesis
Language:	English English
Published:	1997
Subjects:	Lipase Aspergillus flavus Microbial biotechnology
Online Access:	http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf
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id	my-upm-ir.11837
record_format	uketd_dc
spelling	my-upm-ir.118372012-10-29T07:50:47Z Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link 1997-09 Long, Kamariah One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (naturally immobilize) and work using it, is limited to a few microorganisms. This study was conducted with the aims to identifying a new source of mycelium-bound lipase, and consequently to study it's production, properties and applications.Aspergillus flavus, isolated from copra meal, produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6.5 and with 2% (w/v) each of com oil and yeast extract as carbon and organic nitrogen sources, respectively. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. The presence of EDTA improved the productivity of the mycelium-bound lipase by 26% although growth of A. flavus was inhibited by 11%. The addition of Tween 80, into culture medium, decreased the activity of mycelium-bound lipase. Lipase Aspergillus flavus Microbial biotechnology 1997-09 Thesis http://psasir.upm.edu.my/id/eprint/11837/ http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Lipase Aspergillus flavus Microbial biotechnology Faculty of Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Lipase Aspergillus flavus Microbial biotechnology
spellingShingle	Lipase Aspergillus flavus Microbial biotechnology Long, Kamariah Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
description	One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (naturally immobilize) and work using it, is limited to a few microorganisms. This study was conducted with the aims to identifying a new source of mycelium-bound lipase, and consequently to study it's production, properties and applications.Aspergillus flavus, isolated from copra meal, produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6.5 and with 2% (w/v) each of com oil and yeast extract as carbon and organic nitrogen sources, respectively. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. The presence of EDTA improved the productivity of the mycelium-bound lipase by 26% although growth of A. flavus was inhibited by 11%. The addition of Tween 80, into culture medium, decreased the activity of mycelium-bound lipase.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Long, Kamariah
author_facet	Long, Kamariah
author_sort	Long, Kamariah
title	Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_short	Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_full	Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_fullStr	Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_full_unstemmed	Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_sort	production, properties and applications of mycelium-bound lipase of a locally isolated strain of aspergillus flavus link
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	1997
url	http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf
_version_	1747811271481229312

Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link

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