Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein compone...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2002
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf |
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| Summary: | Retrotransposons are the most common class of eukaryotic transposable elements
that make up over 50% of nuclear DNA found in many plants with large and
complex genomes. The long terminal repeat (LTR) retrotransposons have a high
degree of autonomy and encode at least five distinct protein components, which are
required for their movement in the genome. We have exploited the repetitive,
dispersed nature of many LTR-containing retrotransposon families for the
visualization of genomic polymorphism (genome constitution) and the visualization
of varieties polymorphism (varieties classification). The IRAP-PCR (Inter-
Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are
generated by the proximity of two LTRs or reverse transcriptase CRT) genes using
outward-facing primers annealing to LTR target sequence. This method could
distinguish between banana genome constitution (A and B genome) and varieties.
This method can track new insertion of retrotransposon in banana tissue culture
materials. The evolution of Malaysian bananas can also be tracked using this
phylogenetic analysis. Since the method is PCR-based, it may allow the detection
and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material.
This method is applicable to other plants with dispersed families of LTR retrotransposons
where either the LTR or nearby internal sequences are known. |
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