Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia

Modified FDA method was found to give higher recovery of Listeria spp. than USDA method for different sources. The results indicated that the imported frozen beef samples from wet market examined were contaminated by seven different Listeria spp. namely, L. monocytogenes, L. ivanovii, L. seelige...

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Main Author:	Rahayuningsih, Hajjah Endang purwati
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Listeriosis in animals - Malaysia. Listeria - Susceptibility - Malaysia.
Online Access:	http://psasir.upm.edu.my/id/eprint/11880/1/FSMB_2003_36.pdf
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id	my-upm-ir.11880
record_format	uketd_dc
spelling	my-upm-ir.118802024-06-27T02:41:49Z Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia 2003-01 Rahayuningsih, Hajjah Endang purwati Modified FDA method was found to give higher recovery of Listeria spp. than USDA method for different sources. The results indicated that the imported frozen beef samples from wet market examined were contaminated by seven different Listeria spp. namely, L. monocytogenes, L. ivanovii, L. seeligeri, L. innocua, L . welshimeri, L. grayi and L. murrayi. However, the use of FDA, USDA and the modified USDA methods may be more beneficial where a limited range of Listeria spp. to be recovered (L. monocytogenes, L. ivanovii and L . innocua). Listeria spp. was not isolated from any of the 23 samples of imported frozen beef from supermarket and from the five samples of buffalo meat examined. A total of two hundred and seventy isolates of Listeria spp. from different sources were investigated for their susceptibility to 18 antibiotics and were screened for plasmid profiles. Antibiotic susceptibility analysis revealed that all Listeria spp. isolates were resistant to two or more antibiotic (MAR 0 . 11 to 0.66). Majority of the 52 isolates of L. monocytogenes displayed resistance to bacitracin , ceptazidime, nalidixic acid , sulfamethazole. However, none were resistant to norfloxacin. Plasmid was detected in 9 (81.8%) of 11 strains of L. murrayi, 12 (80%) of 15 strains of L . grayi, 29 (55.8%) of 52 strains of L. monocytogenes, 8(50%) of 16 strains of L. denitrificans, 25 (41 .7%) of 60 strains of L. ivanovii, 25 (37.9%) of 66 strains of L.innocua), 13 (31 %) of 42 strains of L. welshimeri and 1 (1 2.5% ) of 8 strains of L. seeligeri. The plasmid sizes ranged from 2.7 to 54 Kb. Listeriosis in animals - Malaysia. Listeria - Susceptibility - Malaysia. 2003-01 Thesis http://psasir.upm.edu.my/id/eprint/11880/ http://psasir.upm.edu.my/id/eprint/11880/1/FSMB_2003_36.pdf text en public doctoral Universiti Putra Malaysia Listeriosis in animals - Malaysia. Listeria - Susceptibility - Malaysia. Faculty of Food Science and Biotechnology Hassan, Zaiton English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Hassan, Zaiton
topic	Listeriosis in animals - Malaysia. Listeria - Susceptibility - Malaysia.
spellingShingle	Listeriosis in animals - Malaysia. Listeria - Susceptibility - Malaysia. Rahayuningsih, Hajjah Endang purwati Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
description	Modified FDA method was found to give higher recovery of Listeria spp. than USDA method for different sources. The results indicated that the imported frozen beef samples from wet market examined were contaminated by seven different Listeria spp. namely, L. monocytogenes, L. ivanovii, L. seeligeri, L. innocua, L . welshimeri, L. grayi and L. murrayi. However, the use of FDA, USDA and the modified USDA methods may be more beneficial where a limited range of Listeria spp. to be recovered (L. monocytogenes, L. ivanovii and L . innocua). Listeria spp. was not isolated from any of the 23 samples of imported frozen beef from supermarket and from the five samples of buffalo meat examined. A total of two hundred and seventy isolates of Listeria spp. from different sources were investigated for their susceptibility to 18 antibiotics and were screened for plasmid profiles. Antibiotic susceptibility analysis revealed that all Listeria spp. isolates were resistant to two or more antibiotic (MAR 0 . 11 to 0.66). Majority of the 52 isolates of L. monocytogenes displayed resistance to bacitracin , ceptazidime, nalidixic acid , sulfamethazole. However, none were resistant to norfloxacin. Plasmid was detected in 9 (81.8%) of 11 strains of L. murrayi, 12 (80%) of 15 strains of L . grayi, 29 (55.8%) of 52 strains of L. monocytogenes, 8(50%) of 16 strains of L. denitrificans, 25 (41 .7%) of 60 strains of L. ivanovii, 25 (37.9%) of 66 strains of L.innocua), 13 (31 %) of 42 strains of L. welshimeri and 1 (1 2.5% ) of 8 strains of L. seeligeri. The plasmid sizes ranged from 2.7 to 54 Kb.
format	Thesis
qualification_level	Doctorate
author	Rahayuningsih, Hajjah Endang purwati
author_facet	Rahayuningsih, Hajjah Endang purwati
author_sort	Rahayuningsih, Hajjah Endang purwati
title	Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
title_short	Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
title_full	Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
title_fullStr	Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
title_full_unstemmed	Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia
title_sort	molecular characterization of listeria spp. isolated from beef, chicken and fermented fish in malaysia
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Biotechnology
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/11880/1/FSMB_2003_36.pdf
_version_	1804888665106677760

Molecular Characterization Of Listeria Spp. Isolated From Beef, Chicken And Fermented Fish In Malaysia

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