Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb

Preliminary screening for in vitro cytotoxic and inhibition nitric oxide (NO) production acivities were carried out on fourteen extracts of different parts of eight Garcinia species. These extracts were evaluated for cytotoxic activity using microculture tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-...

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Main Author:	Sri Wahyuni, Fatma
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Pharmacology Guttiferae
Online Access:	http://psasir.upm.edu.my/id/eprint/12057/1/IB_2009_14_A.pdf
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id	my-upm-ir.12057
record_format	uketd_dc
spelling	my-upm-ir.120572013-05-27T07:50:56Z Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb 2009-08 Sri Wahyuni, Fatma Preliminary screening for in vitro cytotoxic and inhibition nitric oxide (NO) production acivities were carried out on fourteen extracts of different parts of eight Garcinia species. These extracts were evaluated for cytotoxic activity using microculture tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring the reduction of viability of cell cultures in the presence and absence of the extracts. Four type of cancer cell lines, HL-60 (leukemia), MCF-7 (breast), DU-145 (prostate) and NCI-H460 (non-small cell lung) were used for a preliminary picture of extracts selectivity profile. NO inhibition (in lipopolysaccharide (LPS)-activated macrophages) activity was evaluated using Griess assay. Extract of the stem bark from G. cowa Roxb showed selective cytotoxicity towards NCI- H460 (IC50 = 11 μg/mL). The extract also exhibited inhibition of NO production (IC50= 25 μg/mL) without being cytotoxic to RAW 264.7 cells. Based on these promising selective cytotoxicity towards NCI-H460 and NO inhibitory activities, G. cowa was selected for further studies on the isolation and identification its active components. Pharmacology Guttiferae 2009-08 Thesis http://psasir.upm.edu.my/id/eprint/12057/ http://psasir.upm.edu.my/id/eprint/12057/1/IB_2009_14_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Pharmacology Guttiferae Faculty Of Bioscience English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Pharmacology Guttiferae
spellingShingle	Pharmacology Guttiferae Sri Wahyuni, Fatma Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
description	Preliminary screening for in vitro cytotoxic and inhibition nitric oxide (NO) production acivities were carried out on fourteen extracts of different parts of eight Garcinia species. These extracts were evaluated for cytotoxic activity using microculture tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring the reduction of viability of cell cultures in the presence and absence of the extracts. Four type of cancer cell lines, HL-60 (leukemia), MCF-7 (breast), DU-145 (prostate) and NCI-H460 (non-small cell lung) were used for a preliminary picture of extracts selectivity profile. NO inhibition (in lipopolysaccharide (LPS)-activated macrophages) activity was evaluated using Griess assay. Extract of the stem bark from G. cowa Roxb showed selective cytotoxicity towards NCI- H460 (IC50 = 11 μg/mL). The extract also exhibited inhibition of NO production (IC50= 25 μg/mL) without being cytotoxic to RAW 264.7 cells. Based on these promising selective cytotoxicity towards NCI-H460 and NO inhibitory activities, G. cowa was selected for further studies on the isolation and identification its active components.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Sri Wahyuni, Fatma
author_facet	Sri Wahyuni, Fatma
author_sort	Sri Wahyuni, Fatma
title	Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
title_short	Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
title_full	Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
title_fullStr	Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
title_full_unstemmed	Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb
title_sort	isolation,characterization and preliminary pharmacological evaluation of constituents of garcinia cowa roxb
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Of Bioscience
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/12057/1/IB_2009_14_A.pdf
_version_	1747811295926681600

Isolation,Characterization And Preliminary Pharmacological Evaluation Of Constituents Of Garcinia Cowa Roxb

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