Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry

The use of probiotics as an alternative to antibiotic growth promoter has gained popularity in the commercial poultry industry in view of the hazards posed by antibiotics to human and animal health. However, the science behind the probiotic microorganisms has been poor with respect to their ident...

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Main Author:	Lee, Chin Mei
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Probiotics Lactobacillus - Molecular aspects Poultry
Online Access:	http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf
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id	my-upm-ir.12059
record_format	uketd_dc
spelling	my-upm-ir.120592013-05-27T07:50:56Z Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry 2009-12 Lee, Chin Mei The use of probiotics as an alternative to antibiotic growth promoter has gained popularity in the commercial poultry industry in view of the hazards posed by antibiotics to human and animal health. However, the science behind the probiotic microorganisms has been poor with respect to their identity at the species and strain level, their interaction with the host animal, and their efficacy in poultry practices. Thus, a reliable and efficient method is essential to monitor the probiotic microorganisms and to perform quality control of commercial probiotic products. In the present study, molecular methods were applied for reidentification, characterisation and enumeration of 12 probiotic Lactobacillus strains which were previously identified with classical biochemical tests. Based on comparative sequence analyses of the 16S ribosomal RNA (rRNA) gene and 16S-23S rRNA gene intergenic spacer region (ISR), discrepancies were found in the identification of nine out of the 12 Lactobacillus strains, namely, L. brevis C 1, L. brevis C 10, L. fermentum C 16, L. brevis C 17, L. crispatus I12, L. acidophilus I 16, L. fermentum I 24, L. fermentum I 25 and L. acidophilus I 26. These strains were reidentified as L. reuteri C 1, L. reuteri C 10, L. reuteri C 16, L. panis C 17, L. brevis I 12, L. gallinarum I 16, L. salivarius I 24, L. brevis I 25 and L. gallinarum I 26. The rate of misidentification is high when conventional identification methods are used. Probiotics Lactobacillus - Molecular aspects Poultry 2009-12 Thesis http://psasir.upm.edu.my/id/eprint/12059/ http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf application/pdf en public masters Universiti Putra Malaysia Probiotics Lactobacillus - Molecular aspects Poultry Institute of Bioscience English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Probiotics Lactobacillus - Molecular aspects Poultry
spellingShingle	Probiotics Lactobacillus - Molecular aspects Poultry Lee, Chin Mei Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
description	The use of probiotics as an alternative to antibiotic growth promoter has gained popularity in the commercial poultry industry in view of the hazards posed by antibiotics to human and animal health. However, the science behind the probiotic microorganisms has been poor with respect to their identity at the species and strain level, their interaction with the host animal, and their efficacy in poultry practices. Thus, a reliable and efficient method is essential to monitor the probiotic microorganisms and to perform quality control of commercial probiotic products. In the present study, molecular methods were applied for reidentification, characterisation and enumeration of 12 probiotic Lactobacillus strains which were previously identified with classical biochemical tests. Based on comparative sequence analyses of the 16S ribosomal RNA (rRNA) gene and 16S-23S rRNA gene intergenic spacer region (ISR), discrepancies were found in the identification of nine out of the 12 Lactobacillus strains, namely, L. brevis C 1, L. brevis C 10, L. fermentum C 16, L. brevis C 17, L. crispatus I12, L. acidophilus I 16, L. fermentum I 24, L. fermentum I 25 and L. acidophilus I 26. These strains were reidentified as L. reuteri C 1, L. reuteri C 10, L. reuteri C 16, L. panis C 17, L. brevis I 12, L. gallinarum I 16, L. salivarius I 24, L. brevis I 25 and L. gallinarum I 26. The rate of misidentification is high when conventional identification methods are used.
format	Thesis
qualification_level	Master's degree
author	Lee, Chin Mei
author_facet	Lee, Chin Mei
author_sort	Lee, Chin Mei
title	Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
title_short	Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
title_full	Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
title_fullStr	Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
title_full_unstemmed	Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
title_sort	molecular approaches for identification, characterisation and quantification of probiotic lactobacillus strains for poultry
granting_institution	Universiti Putra Malaysia
granting_department	Institute of Bioscience
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf
_version_	1747811296415318016

Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry

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