In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats

Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolyt...

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Main Author:	Mat Amin, Maswati
Format:	Thesis
Language:	English English
Published:	1998
Subjects:	Goats - Diseases Sheep - Diseases
Online Access:	http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf
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id	my-upm-ir.12187
record_format	uketd_dc
spelling	my-upm-ir.121872011-09-07T02:15:17Z In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats 1998-07 Mat Amin, Maswati Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolytica A2 revealed an early colonization of the bacteria onto the lung tissue as early as 1 hour post-challenge. The severity of colonization increased with time of challenge and reached a maximum rate at 6 hours post-challenge . Similar in vitro challenge on the lung tissues derived from goats that were exposed earlier to intranasal sprays of formalin-killed Pasteurella haemolytica A2, however, revealed a less severe colonization by 6 hours postchallenge. Following intratracheal challenge of goats with 108/ml colony forming units of Pasteurella haemolytica A2, 20% of the goats succumbed to peracute infection in which they died within 12 hours post-challenge. Examinations of the lungs revealed classical toxaemic lesions consisted of severe pulmonary oedema, pulmonary congestion and haemorrhage, and thrombosis with a few neutrophils in the alveoli. The lesions were remarkably similar to those peracute infections caused by Pasteurella multocida types A and D. The only difference was the absence of Pasteurella haemolytica A2 organisms in the heart blood samples compared to the infections by Pasteurella multocida types A and D. Goats that survived the peracute episode developed pneumonic lesion. Phagocytic activity by the bronchoalveolar macrophages was obvious by 4 days postchallenge and by day 7 post-challenge, the goats which were unable to phagocytose most of the bacterial cells succumbed to severe pneumonia in which the bacteria proliferated and overloaded the lungs leading to the invasion of the bacteria into the pneumocytes and spreading the infection further. Goats with efficient phagocytosis were able to reduce the number of bacterial cells in the lungs leading to failure of bacterial establishment in the lungs. Infections by Pasteurella haemolytica A2 isolated from nasal mucosa produced an insignificantly (p>0.05) less extensive lung lesions compared to infections by Pasteurella haemolytica A2 isolated from pneumonic lungs. The pulmonary responses and pattern of lesion developrrient, however, remained similar for both isolates. Goats - Diseases Sheep - Diseases 1998-07 Thesis http://psasir.upm.edu.my/id/eprint/12187/ http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf application/pdf en public masters Universiti Putra Malaysia Goats - Diseases Sheep - Diseases Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Goats - Diseases Sheep - Diseases
spellingShingle	Goats - Diseases Sheep - Diseases Mat Amin, Maswati In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
description	Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolytica A2 revealed an early colonization of the bacteria onto the lung tissue as early as 1 hour post-challenge. The severity of colonization increased with time of challenge and reached a maximum rate at 6 hours post-challenge . Similar in vitro challenge on the lung tissues derived from goats that were exposed earlier to intranasal sprays of formalin-killed Pasteurella haemolytica A2, however, revealed a less severe colonization by 6 hours postchallenge. Following intratracheal challenge of goats with 108/ml colony forming units of Pasteurella haemolytica A2, 20% of the goats succumbed to peracute infection in which they died within 12 hours post-challenge. Examinations of the lungs revealed classical toxaemic lesions consisted of severe pulmonary oedema, pulmonary congestion and haemorrhage, and thrombosis with a few neutrophils in the alveoli. The lesions were remarkably similar to those peracute infections caused by Pasteurella multocida types A and D. The only difference was the absence of Pasteurella haemolytica A2 organisms in the heart blood samples compared to the infections by Pasteurella multocida types A and D. Goats that survived the peracute episode developed pneumonic lesion. Phagocytic activity by the bronchoalveolar macrophages was obvious by 4 days postchallenge and by day 7 post-challenge, the goats which were unable to phagocytose most of the bacterial cells succumbed to severe pneumonia in which the bacteria proliferated and overloaded the lungs leading to the invasion of the bacteria into the pneumocytes and spreading the infection further. Goats with efficient phagocytosis were able to reduce the number of bacterial cells in the lungs leading to failure of bacterial establishment in the lungs. Infections by Pasteurella haemolytica A2 isolated from nasal mucosa produced an insignificantly (p>0.05) less extensive lung lesions compared to infections by Pasteurella haemolytica A2 isolated from pneumonic lungs. The pulmonary responses and pattern of lesion developrrient, however, remained similar for both isolates.
format	Thesis
qualification_level	Master's degree
author	Mat Amin, Maswati
author_facet	Mat Amin, Maswati
author_sort	Mat Amin, Maswati
title	In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_short	In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_full	In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_fullStr	In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_full_unstemmed	In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_sort	in vitro and in vivo establishment of pasteurella haemolytica a2 in the lungs of goats
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	1998
url	http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf
_version_	1747811324287516672

In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats

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