Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)

Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedure...

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Main Author: Abdullah, Mohamad Hilmi
Format: Thesis
Language:English
English
Published: 1984
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf
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spelling my-upm-ir.122632024-07-10T08:04:31Z Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) 1984-02 Abdullah, Mohamad Hilmi Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement. Water buffalo - Cytogenetics. 1984-02 Thesis http://psasir.upm.edu.my/id/eprint/12263/ http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf text en public doctoral Universiti Pertanian Malaysia Water buffalo - Cytogenetics. Faculty of Veterinary medicine Bongso, Tuan Ariffeen English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Bongso, Tuan Ariffeen
topic Water buffalo - Cytogenetics.


spellingShingle Water buffalo - Cytogenetics.


Abdullah, Mohamad Hilmi
Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
description Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement.
format Thesis
qualification_level Doctorate
author Abdullah, Mohamad Hilmi
author_facet Abdullah, Mohamad Hilmi
author_sort Abdullah, Mohamad Hilmi
title Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
title_short Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
title_full Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
title_fullStr Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
title_full_unstemmed Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
title_sort cytogenetic studies on the water buffalo (bubalus bubalis)
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Veterinary medicine
publishDate 1984
url http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf
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