Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic prop...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1992
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12299/1/FPV_1992_3_A.pdf |
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| Summary: | This research was carried out to investigate two isolates
of bovine herpes virus I (BHV -l) isolated from buffaloes at
Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan,
and compare them to a reference cattle strain of BHV- l ( Cooper
strain) .
The cytopathogenic and serologic properties of the three
virus isolated (Buffalo respiratory , Buffalo genital , and the
Cooper strain) were first studied. The cytopathogenicity of
the three BHV-1 isolates was investigated using Acridine Orange
staining (to compare localization of DNA) , Hematoxylin and
Eosin staining (to compare morphologic changes induced in cell
culture), the indirect Immunoperoxidase ( lIP) staining method ( to compare localization o f the virus antigen ) , plaque assay,
and Negative Contrast Electron Microscopy. |
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